Lots of assays are routinely used to assess hair growth, while hair follicle organ culture model is one of the most popular and influential in vitro systems 1. Rodent vibrissa follicles are regular, predictable, and relatively short growth cycles. Hence, considering these properties, authors choose mouse vibrissa follicles as a test model for the assessment of hair cycle and compared morphologic changes in culture 2. With the measurement of follicular activity concerning bulb thickness and improvement of anagen initiation, regression of catagen, and finally shifting of hair bulb i.e., telogen formation is the main criteria for hair growth 3. The hair follicle is consist of mainly two components one is epithelial components, and the others are dermal components. Hair growth is regulated by the division of the hair follicle matrix cells under control of the dermal papilla. Three different stages of hair growth can be identified, an active phase (anagen) during which hair growth occurs, an intermediate regressive (catagen) stage and a resting phase (telogen) during which no cell proliferation occurs 4.The positive control used in this experiment i.e., minoxidil because from literature reported that it can directly promote hair growth via the stimulation of growth factor release from adipose-derived stem cells dermal papilla and epithelial cells 5. In recent years, several scientific reports and clinical trials have revealed the beneficial effects of Biofield Energy Treatments, which have shown to enhance immune function in cases of cervical cancer patients via therapeutic touch 6, massage therapy 7, etc. Complementary and Alternative Medicine (CAM) therapies are now rising as preferred models of treatment, among which Biofield Therapy (or Healing Modalities) is one approach that has been reported to have several benefits to enhance physical, mental and emotional human wellness. However, as per the data of 2012 from the National Health Interview Survey (NHIS), which indicated that the highest percentage (17.7%) of the Americans used dietary supplements as a complementary health approach as compared with other practices in past years. The National Center of Complementary and Integrative Health (NCCIH) has recognized and accepted Biofield Energy Healing as a CAM health care approach in addition to other therapies, medicines and practices such as natural products, deep breathing, yoga, Tai Chi, Qi Gong, chiropractic/osteopathic manipulation, meditation, massage, special diets, homeopathy, progressive relaxation, guided imagery, acupressure, acupuncture, relaxation techniques, hypnotherapy, healing touch, movement therapy, pilates, rolfing structural integration, mindfulness, Ayurvedic medicine, traditional Chinese herbs and medicines, naturopathy, essential oils, aromatherapy, Reiki, and cranial sacral therapy. Human Biofield Energy has subtle energy that can work efficiently 8. CAM therapies have been practiced worldwide with reported clinical benefits in different health disease profiles 9. This energy can be harnessed and transmitted by the experts into living and non-living things via the process of Biofield Energy Healing. Biofield Energy Treatment (The Trivedi Effect®) has been published in numerous peer-reviewed science journals with significant outcomes in many scientific fields such as cancer research 10, 11, microbiology 12, 13, 14, 15, biotechnology 16, 17, pharmaceutical science 18, 19, 20, 21, agricultural science 22, 23, 24, 25, materials science 26, 27, 28, 29, nutraceuticals 30, 31, skin health, human health and wellness.
Based on the literature information and importance of Biofield Energy Healing Treatment on various fields, the authors sought to evaluate the impact of the Biofield Energy Treatment (The Trivedi Effect®) on the test item (William’s Medium E) for hair cells growth activity with respect to the assessment of different hair growth parameters like bulb thickness and telogen formation using standard assays in vibrissae hair follicle organ culture cells with the help of UTHSCSA Image tool version 3.