Journal of Experimental and Clinical Toxicology

Journal of Experimental and Clinical Toxicology

Journal of Experimental and Clinical Toxicology

Current Issue Volume No: 1 Issue No: 2

Research Article Open Access Available online freely Peer Reviewed Citation

Biolarvicidal Potentials of the Methanolic-Leaf-Extracts of Selected Tropical Plant Species

1Department of Biological Sciences, Niger Delta University, Wilberforce Island, Nigeria


The global impact of malaria and challenges encountered during its control have necessitated the application of multifaceted strategies, including the application of plant-derived agents. Amidst these challenges the proliferation of the vector is becoming hyperendemic in tropical region. This research is focused on the biolarvicidal activities of the methanolic leaf-extracts of Cassia alata, Microdesmispuberula¸ and Spilanthesfilicaulis against mosquito larva. The mosquito larva were assayed in a static non-renewal test. Results showed no mortality for the negative control, and total mortality for the positive control (p<0.05). The C. alata bioassay was the most active with LC50 value of 13.73 ppm, followed by; M. puberula(21.24 ppm), and S. filicaulis (28.86 ppm). This study concludes that methanolic-leaf-extracts of C. alata, M. puberula¸ and S. filicaulis can be recommended for the formulation of biolarvicide for the control of malaria.

Author Contributions
Received 25 Mar 2019; Accepted 29 Mar 2019; Published 01 Apr 2019;

Academic Editor: Wei Wu, Nanjing Medical University, China

Checked for plagiarism: Yes

Review by: Single-blind

Copyright ©  2019 Patrick B. Solomon, et al.

Creative Commons License     This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Competing interests

The authors have declared that no competing interests exist.


Patrick B. Solomon, Ayodele A. Oyedeji, Frankland O. Briyai, Dorcas D. S. Bawo, Jasper F.N Abowei (2019) Biolarvicidal Potentials of the Methanolic-Leaf-Extracts of Selected Tropical Plant Species. Journal of Experimental and Clinical Toxicology - 1(2):7-11.

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DOI 10.14302/issn.2641-7669.ject-19-2730


Malaria is a vector-borne disease, which is transmitted by female arthropod (mosquito) belonging to the Anopheles genus1, 2. The disease is prevalent in Africa, with documented information in literature indicating, the Anopheles gambiae species as the predominant transmitter3. It is documented in literature that mosquitoes transmit more diseases, compared to other arthropods 4. Statistical data in literature showed that there are over 40 genera of mosquitoes distributed into over 3,000 species, out of which only about 30 - 40 species transmit malaria in nature 2, 5.

Several metabolites have been identified from various plants, including over 10,000 alkaloids and 25,000 terpenes derivatives 6. Notwithstanding, the therapeutic efficacies in these applied metabolites varies upon some compounding parameters; including, seasonal influence, location, age, individual susceptibility and environmental stresses on the plant 7, or the applied part of the plant such as root, stem, fruits, leaves, and seeds 8, as well as the applied solvent medium used for the plant extraction 9.

The efficacy of C. alata have been documented in literature for the treatment of constipation, stomach pain, and ringworm 10. Microdesmispuberula was known for the treatment of gonorrhoea and erectile dysfunction 11. The antibacterial properties of Spilanthesfilicaulisleaf decoction have also been reported 12.

The application of synthetic therapy like drug administration can only abate morbidity burden and reinfection frequency 13, while pesticides poses potential ecotixicity 9. Due to the prevalence of malaria and search for eco-friendly control measures, multifaceted strategies have become necessary, including researches and application of plant-derived methods 2. Therefore, the investigation on the larvicidal potential of Cassia alata have become necessary.

Material and Method

Collection and Preparation of Plant Samples

Fresh leaves of C. alata, M. puberula and S. filicauliswere collected around the vegetation of Wilberforce Island in Southern Ijaw Local Government Area of Bayelsa State, Nigeria. All plants were identified and washed with de-chlorinated water in the laboratory. Afterward, they were shade-dried at room temperature for 7 days. The shade-dried plants were placed in oven at 50°C for 30 minutes 2. Before the solvent-extraction, the shade-dried leaves were distinctively powdered with electric blender.

Extraction Process

Three hundred grams (300 g) of the powdered leaves of each plants were weighed using Satoric AG Gottingen Electronic weighing balance. The weighed powdered leaves were distinctively macerated in 500 ml of the respective solvents (Chloroform, Hexane and Methanol) for 72 hours and filtered into conical flask using whatman No.1 filter paper 14. The filtrates distinctly extracted using a rotary evaporator at 60°C. The residue of the extracts was allowed to cool and stored at room temperature.

Mosquito Larva Collection

Mosquito Larvae used for this bioassay were cultured in the wild using methods as described by some authors 3, 8, 9, 12, with slight modification. Plastic containers and automobile tyres half-filled with stagnant water, and sand were kept in vegetation of conspicuous breeding sites. Prior to the laboratory bioassay, the larvae were placed on enamel tray and acclimatized to laboratory condition.

Experimental Setup

A minimum of 10 larvae, were distinctly placed in a 500 ml solution of the methanolic-extract at varying concentrations, in a 24-hour static non-renewal test. The bioassay was performed with the standard of the World Health Organization guidelines 12, 15. Mortality rates (%), of larvae was recorded after the period of exposure (24 hours). A concentration of 1 ppm of Dipex pesticide was used as the positive control, while 500 ml of distilled water was used as the negative control. The larvicidal screening protocols was two-phased, involving the rapid and final Screening 12.

Statistical Analysis

The data for mortality rates were expressed as mean± standard deviation using version 20 of SPSS statistical package. A one-way analysis of variance was used to carry out the statistical analysis, while Duncan multiple range test was used to determine the source of observed difference using SPSS Version 20.

Results and Discussion

The mortality rates of all methanolic leaf extracts of the plants (C. alata, M. puberula and S. filicaulis), assayed against the larvae is presented in Table 1. Results showed that the positive control had total mortality at concentration below 10.00 ppm, while the negative control demonstrated no mortalities against the vectors (Table 1). For the Cassia alata bioassay results showed that mortality rate increased significantly with higher concentration (p<0.05). However, mortality rates ranging from 46.66 – 100.00%, with the minimal and total minimal mortality rates at concentrations 10 and 60 ppm respectively (Table 1).

The no adverse effect level was observed at concentration of 0 ppm. Furthermore, based on dose-response curve the Cassia alata bioassay had biolarvicidal activity with LC50 value of 13.72 ppm (Figure 1). The result for the methanolic-leaf-extract bioassay of M. puberula showed that mortality rate significantly ranged from 30.00 – 100.00% as presented in Table 1. The minimal mortality rate was observed at concentration of 10 ppm, while the total minimal mortality rate was observed at concentration of 70 ppm Table 1. Meanwhile no adverse effect level was at concentration of 0 ppm Table 1. Based on the dose-response the activity of methanolic leaf extract of M. puberula was demonstrated with LC50 value of 21.24 ppm (Figure 1). Results of the S. filicaulis methanolic-leaf-extract showed that mortality rates ranged from 16.66 – 100.00% (p<0.05). The minimal and total minimal concentration were 10 and 80 ppm respectively Table 1, with LC50 value of 28.86 ppm (Figure 1).

Table 1. Biolarvicidal Mortality rates of Methanolic-leaf-extracts
Conc. Mortality Rates (%)
C. alata M. puberula S. filicaulis Positive Negative
0 ppm 0.00±0.00a 0.00±0.00a 0.00±0.00a 0.00±0.00a 0.00±0.00a
10 ppm 46.66±11.54b 30.00±10.00b 16.66±5.77b 100.00±0.00e 0.00±0.00a
20 ppm 56.66±5.77bc 43.33±5.77c 33.33±5.77c 100.00±0.00e 0.00±0.00a
30 ppm 63.33±5.77cd 53.33±15.27c 43.33±11.54d 100.00±0.00e 0.00±0.00a
40 ppm 73.33±11.54d 66.66±5.77d 53.33±5.77e 100.00±0.00e 0.00±0.00a
50 pm 90.00±10.00e 80.00±10.00e 66.67±5.77f 100.00±0.00e 0.00±0.00a
60 ppm 100.00±0.00e 96.66±5.77f 76.66±5.77g 100.00±0.00e 0.00±0.00a
70 ppm 100.00±0.00e 100.00±0.00f 93.33±5.77h 100.00±0.00e 0.00±0.00a
80 ppm 100.00±0.00e 100.00±0.00f 100.00±0.00h 100.00±0.00e 0.00±0.00a
90 ppm 100.00±0.00e 100.00±0.00f 100.00±0.00h 100.00±0.00e 0.00±0.00a
100 ppm 100.00±0.00e 100.00±0.00f 100.00±0.00h 100.00±0.00e 0.00±0.00a

Data expressed as mean ± standard deviation, differences in alphabetical subscript indicates significance in mortality

Figure 1.Biolarvicidal dose-response for methanolic-leaf-extracts of selected plant species
 Biolarvicidal dose-response for methanolic-leaf-extracts of selected plant species

Results of this study is comparable to a recent study for the larvicidal methanolic leaf (32.13 – 100.00%) and Hexane flower of S. alata (21.94 – 100.00%) of Senna alata (16). The comparative larvicidal efficacies of the leaves, bark, stem and root of Jatropha curcas(Euphorbiaceae) against 3rd and 4th instar larvae Anopheles gambiae was reported by Ohimain et al., 9. Results of their studies demonstrated activities with LC100 and LC50 values for crude extract of the bark (437.5 and 87.5 ppm), methanolic-extract of the root (312.5 and 62.5 ppm), methanolic-extracts of the stem (237.5 and 47.5 ppm), leaves (75 and 15 ppm), and the bark (30 and 6 ppm), which was the most active.

The larvicidal activities of solvent-extracts of Hyptissuavolens and Ocimun sanctum were investigated against mosquito larva by Ohimain et al., 17. Results showed that the methanolic-extract of H. suaveolensinduced was the most active with LC50 values of 73.25 ppm, followed by the chloroform and hexane extracts that demonstrated activities with LC50 values of 76.25 and 97.25 ppm respectively. Comparatively, the O. sanctum bioassay demonstrated activities LC50 values of 125.00 for methanolic-extract, 150.00 for chloroform-extract and 194.08 ppm for hexane-extract.

Several authors have reported phytochemicals in C. alata, M. puberula, and S. filicaulis that supported their antimicrobial activities 18, 19. Phytochemical like alkaloid, flavonoid, saponin, tannin and phenol were predominantly found in the leaves of C. alata20. Several author have confirmed the phytochemistry of M. puberula, this include phytochemicals like; alkaloid, flavonoid, saponin and steroids 20. The activities of S. filicaulis have been ascribed to predominant phytochemicals such as athraquinones, alkaloid, flavonoid, tannin and phenol 15, 19, 20.


This study investigated the biolarvicidal potential of the methanolic extracts of C. alata, M. puberula¸ and S. filicaulis. Intrestingly, all extracts of the plant showed larvicidal activities with the C. alata extract exhibiting higher mortality, followed by S. filicaulis and M. puberula. Solvent methanolic-extracts of these plants, are hereby recommended for the formulation of biolarvicide for the control of malaria.


The following authors wish to acknowledge Tariwari C.N Angaye for his technical assistant during the laboratory and field work.


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  1. 1.Ojo Olusesan, Olusola Ruth Etiosa, Ojo Oluwabukola Oluwafunmilayo, 2024, Spilanthes filicaulis (Schumach. &Thonn.) C. D. Adams: An insights into ethnopharmacologically important but scientifically understudied species, Annales Pharmaceutiques Françaises, (), 10.1016/j.pharma.2024.05.010