All Articles

Modern Proteomics: Methods and Applications – Special Issue

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Evaluating the Efficacy of Gene Silencing in Dopaminergic Neuronal Cells In-Vitro using Gold Nanorods (GNR) with Different Surface Properties Complexed to DARPP-32 siRNA.

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Gold nanorods (GNRs) are plasmonic nanostructures by virtue of their size-dependent optical properties, offer a bionanotechnology platform in areas of bioimaging, drug delivery etc for disease diagnosis, prognosis, and therapy. GNRs are more sensitive to changes in local environments, and offer strong scattering and absorption efficiencies thus providing opportunities to integrate multiple imaging modes and therapeutic strategies. The hydrodynamic size of these GNR under physiological condition is <100 nm, making them ideal as intracellular delivery agents. RNA interference using small inhibitory RNA (siRNA) has become a powerful tool to downregulate mRNA levels by cellular nucleases that become activated when a sequence homology between the siRNA and a respective mRNA molecule is detected. siRNA is used to silence genes involved in the pathogenesis of various diseases and holds a promising option for the development of novel therapeutic strategies in n...

Phytochemicals May Arrest HIV-1 Progression

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This hypothesis‑driven review considers phytochemicals with putative anti‑HIV activity. It summarizes proposed mechanisms, in vitro evidence, and formulation issues, and calls for standardized assays and clinical trials to determine real‑world efficacy and safety.

Hospital Episodes Due to Antidepressant Overdose: An Under-Utilised Source of Pharmacovigilance Data

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Antidepressant agents are commonly implicated in drug overdose, and the toxicological profile varies between agents. Clinical data concerning overdoses are not systematically sought or evaluated in pharmacovigilance. The present study sought to examine the feasibility of collecting Emergency Department data concerning antidepressant overdose.

Rapid and Sensitive High-Performance Liquid Chromatography/Tandem Mass Spectrometry Method for Determination of Danshensu in Rat Plasma

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A sensitive, specific and rapid high-performance liquid chromatography/tandem mass spectrometry (hplc–ms/ms) method has been developed and validated for the determination of danshensu (dss) in rat plasma in the present study. The analytes were separated on a c18 column (50 mm×2.1 mm, 1.7 μm) and a triplequadrupole mass spectrometry equipped with electrospray ionization (esi) source was applied for detection. The simple protein precipitation was applied to extract dss from the plasma (about 80%). The method was linear over the concentration ranges of 50–1000 ng/ml for dss. The lower limit of quantitation (lloq) of dss was 50 ng/ml. The intra-day and inter-day relative standard deviation (rsd) were less than 15% and the relative error (re) were all within 15%. Finally, the method was successfully applied to support the pharmacokinetic study after guanxinsu solution was orally administrated to the sprague–dawley rat, respectively.

Evaluation of SterculiaUrens Gum as Novel Carrier for Oral Colon Targeted Drug Delivery System

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The purpose of the research is to evaluate Sterculiaurens gum as a carrier for oral colon targeted drug delivery system. Sterculia gum has been reported to have wide pharmaceutical applications such as tablet binder, disintegrant, gelling agent and as a controlled release polymer, but it has not been exploited as colon targeting carrier. For evaluation as a carrier for colonic delivery of drugs characterization of gum was done. Microflora degradation studies of gum were conducted in phosphate buffer solution (PBS) pH 7.4 containing rat caecal content under anaerobic environment. Solubility, swelling index, viscosity and pH of the polymer solution were determined. Different formulation aspects considered were: gum concentration (10–40%), concentration of citric acid (10–30 %) on swelling index and in-vitro drug release. The results of the isothermal stress testing (IST) shows no degradation of samples of model ...

Sotos Syndrome, Isolated Left Ventricular Non Compaction Cardiomyopathy and Ventricular Pre Excitation: A Case Report.

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We report the case of a 22 year-old-male patient affected by Sotos syndrome was preoperatively and urgently evaluated for tracheal stent implantation due to respiratory insufficiency caused by idiopathic tracheal stenosis.

Rest electrocardiogram detected a ventricular pre excitation; a transthoracic echocardiography showed a classic pattern fulfilling criteria for isolated left ventricular non compaction. At the best of our knowledge this is the first report of a patient suffering from Sotos syndrome and simultaneously affected by isolated left ventricular non compaction and ventricular pre excitation.

Late Age at Menarche Increased Common Carotid Artery Intima-Media Thickness in Overweight and Obese Women

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To determine whether age at menarche is an independent predictor of common carotid artery intima-media thickness in overweight and obese adult women.

Sport Activities as a Vehicle for HIV/ AIDS Prevention in Trinidad and Tobago: Organizer’s Perspectives

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Various sporting activities are being used as vehicles for HIV/AIDS education and prevention within the field of sport-for-development (SFD). Kicking AIDS Out! is one SFD program that aims to promote protective attitudes regarding HIV/AIDS amongst youth. This study explored organizers’ perspectives of the use of sporting activities in the Kicking AIDS Out! program in Trinidad and Tobago. Qualitative case study methodology was used to examine the case. Data was generated through semi-structured interviews with seven organizers who were Kicking AIDS Out! staff or volunteer members. Thematic analysis guided data analysis. Generated themes include: Sport to Draw, There’s Something for Everyone, the Emotional Wow, and Beyond Sports. These themes illuminate the idea that sport captivates and attracts youth, and illustrate the use of sport activities to promote engagement in the program as they are tailored to the sk...

Socio-Economic and Demographic Pattern of HIV Occurrence Amongst Attendees in a Tertiary Care Hospital in Eastern India.

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Toanalyze the socio-economic pattern amongst HIV patients in eastern and north-eastern India as measured by different parameters such as educational status, wealth, marital status, sexual behaviour.