All Articles

Lysozyme-Induced Degradation of Chitosan: The Characterisation of Degraded Chitosan Scaffolds

Abstract:

Up till now, chitosan has confirmed its versatile application in skin, cartilage and bone tissue engineering, as well as in drug delivery applications. This study is focused on enzymatic degradation of porous chitosan structures usually designed for mentioned purposes. In vitro degradation was monitored during four weeks of incubation at physiological temperature and in two different media, phosphate buffer saline solution and water. The scaffolds were characterised before and after enzymatic degradation using scanning electron microscopy and infrared spectroscopy with Fourier transformations (FTIR). According to the gravimetric analysis, higher weight loss of chitosan scaffolds was observed in buffered medium with respect to the water. The results implied that the total weight loss obtained in buffer involves physical dissolution of chitosan and lysozyme cleavage of glycoside bond. Importantly, FTIR identification of chitosan scaffolds after enzy...

Pericentric Inversion in Chromosome 10 in a Girl, Inherited from a Phenotypically Normal Mother: Case Report and Literature Review

Abstract:

Pericentric inversions in chromosome 10 are regarded as both common and rare conditions, based on breakage and rearrangement within each specific segment.

We present phenotypic and cytogenetic characterizations of a rare recombinant chromosome 10, namely inv(10)(p11q26), in a 13-month-old flabby girl associated with a maternal pericentric inversion.

A review of the literature on the different aspects of this condition is also provided.

Living Kidney Transplant: The Influence of Intra-Operative Hemodynamics on Delayed Graft Function

Abstract:

Intra-operative insults may subject living kidney transplants to poor outcomes. Therefore, we investigated whether intra-operative recipient and donor hemodynamics could act as predictors of delayed graft function and subsequent outcomes.

Microanatomy of Thalamic Radiations

Abstract:

Thalamic radiations also known as thalamocortical pathways are reciprocal myelinated nerve fibers, arranged in a fanning pattern, grouped into tracts or fasciculi; and connecting the thalamus to the cerebral cortex. Detailed in vitro study of these tracts is seldom reported in the literature.

First Evidences of Epithelial-Mesenchymal Transition and Cancer Stem-Cell Phenotype Acquisition in Dermo-Epidermal Junction of BPV-Infected Neoplasms

Abstract:

Bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis, infectious and neoplastic disease, characterized by the presence of multiple papillomas that can regress spontaneously or to persist and progress to malignancies when in association with environmental cofactors. Although recognized that the BPV can induce DNA damages, the viral role following cancer initiation remains unresolved. Based on this, we stablished cell lines derived from cutaneous papilloma, fibropapilloma and esophageal carcinoma to study the BPV action on epithelial-mesenchymal transition (EMT). Our results showed strong evidences that the virus action can contribute to EMT and, therefore, metastasis.

Reaction Norm of Embryo Growth Rate Dependent on Incubation Temperature in The Olive Ridley Sea Turtle, Lepidochelys Olivacea, from Pacific Central America

Abstract:

Olive ridleys (Lepidochelys olivacea) and loggerheads (Caretta caretta) are two closely phylogenetically related sea turtles that nest in very different thermal habitat. Olive ridleys nest in pan-tropical beaches whereas loggerheads nest in more temperate beaches. In the context of climate change, the temperature in temperate beaches will increase much more than for tropical beach due to buffering effect of air humidity in the later. We have determined the thermal reaction norm for embryonic growth in both species using field records of incubation temperatures and incubation length from loggerheads in Western Mediterranean Sea or olive ridleys from Pacific coast of Guatemala. We show that the optimum temperature for the growth of embryos is lower for loggerheads than for olive ridleys. This makes loggerhead turtles particularly sensitive to increase of beach temperature as it is expected due to effect of global wa...

Spatial Heterogeneity of Soil Respiration Response to Precipitation Pulse in a Temperate Mixed Forest in Central China

Abstract:

Water availability is one of the fundamental drivers for biological activities and terrestrial carbon cycling. Although the response of soil respiration to precipitation has been well documented in arid and semiarid ecosystems, our understanding of its pattern in forests is rather limited. This study was conducted to examine the difference of precipitation effect on soil respiration under different canopy conditions in a temperate coniferous (Pinus armandii Franch) and broadleaved (Quercus aliena var. acuteserrata) mixed forest in Central China. The results showed that precipitation significantly reduced soil temperature, but increased soil volumetric water content and soil respiration (6.0%-35.3%). Precipitation caused a greater increment in soil respiration beneath the canopy of broadleaved trees (24.2%) than that beneath coniferous ones (13.5%). Precipitation-induced increase i...

JALR. New Journal, Old questions, Fresh insights

Abstract:

This editorial for the Journal of Alzheimers Research and Therapy outlines persistent questions in Alzheimer biology and care, and highlights emerging tools and study designs. The journal aims to connect mechanistic insight with therapeutic development and practice. It invites interdisciplinary work to accelerate translation and improve outcomes for patients and caregivers.

Acute and Chronic Wound Fluid Inversely Influence Wound Healing in an in-Vitro 3D Wound Model

Abstract:

If a wound progressively heals or the healing process is impaired is basically influenced by the surrounding milieu. This is reflected by the wound fluid. Its specific composition triggers the migration, proliferation and differentiation of dermal and epidermal cells which so far was not sufficiently examined in 2D cell culture models. The influence of the different wound entities was analyzed on a newly implemented three dimensional in-vitro model, which improved the transferability to the in-vivo situation. The influence of pooled wound fluids from patients suffering from acute or chronic wounds were investigated within a time period of 10 days after wound application. Histological and immunohistochemical analyses were performed addressing the impact of AWF and CWF on regeneration, such as cell proliferation, fibroblast activity and cell migration. AWF slightly stimulated fibroblast migration while CWF inhibited their activation...

This Isn’t Just a Phantom, Menopause, But You Can Vent Your Feelings - Qualitative Evaluation of Evidence Based Health Information (EBHI) Material

Abstract:

Because of the increased demand for the availability of independent information regarding the efficacy, the lack of efficacy and the possible harm of medical interventions, the study aims to evaluate the information package „Menopause", published by the German Institute for Quality in Healthcare (IQWiG).

Methods: Qualitative, guideline-based interviews, carried out in n=41 women (40-63 years). The analysis of transcripts was carried out according to Mayring.