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Journal of Plant and Animal Ecology

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ISSN: 2637-6075
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  • Growth Pattern of Saccharomyces cerevisiae in Cassava Mill Effluents

    Sylvester Chibueze Izah 1  

    1Department of Biological Sciences, Faculty of Science, Niger Delta University, Wilberforce Island,Bayelsa State, Nigeria.


    Nigeria is the world leading producer of cassava. During processing of gari from cassava tuber large volume of effluents are discharged in the environment which is toxic to the environment and some of its associated biota. This study evaluated the growth pattern of Saccharomyces cerevisiae in cassava mill effluents. The Saccharomyces cerevisiae was isolated from palm wine following standard microbiological procedure. The Saccharomyces cerevisiae was inoculated into the sterile effluents and incubated for 15 days. At every 3days interval, 1ml of the effluents was obtained from the medium and the population density determined. Results of the growth showed that the population of Saccharomyces cerevisiae were 0.00 x 106 cfu/ml at day 0 (without inoculum), which rose to 2.88 x 106 cfu/ml at day 3, 272.67 x 106 cfu/ml at day 12 and decline slightly at day 15 (13.57 x 106 cfu/ml). There was significant variations (P<0.05) among the various period of study. The study showed that the growth of Saccharomyces cerevisiae in the effluent was optimum at day 12, then after the density began to decline.

    Author Contributions
    Received 12 Sep 2018; Accepted 28 Sep 2018; Published 02 Oct 2018;

    Academic Editor: Narcisa Vrinceanu, Faculty of Engineering, Lucian Blaga†University of Sibiu /4 Emil Cioran Street, 550025 Sibiu, Romania.

    Checked for plagiarism: Yes

    Review by: Single-blind

    Copyright ©  2018 Sylvester Chibueze Izah

    Creative Commons License     This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Competing interests

    The authors have declared that no competing interests exist.


    Sylvester Chibueze Izah (2018) Growth Pattern of Saccharomyces cerevisiae in Cassava Mill Effluents. Journal of Plant and Animal Ecology - 1(2):10-15.

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    DOI 10.14302/issn.2637-6075.jpae-18-2349


    The level of environmental degradation has been on the increasing trend probably due to human activities on the environment and to lesser extent by natural phenomenon 1, 2, 3, 4, 5. The degradation of the environment (e.g air, soil, sediment and water quality) often affect different life forms in such ecosystem. Authors have reported that human activities is having an impacts on the population status of biodiversity including mammals, amphibians, fisheries, reptiles, birds, vegetation/plants among others 6, 7, 8, 9.

    Several human activities that impact on the soil including municipal solid wastes that are poorly managed in many developing countries 10, food processing effluents such as oil palm processing wastewater 11, 12 and cassava mill effluents 1, 2, 3, wastes emanating from markets 22, 23, 24, slaughterhouse25etc. These wastes stream have the tendency to alter the characteristics of the receiving soil.

    Basically Nigeria is the global leading cassava producing countries 14, 15, 16, 17, 18, 19, 20, 21. The cassava production in Nigeria has exceeded the combined production of second (Thailand) and third (Indonesia) largest cassava production countries in the world. In Nigeria, significant portion of the cassava tuber produced is used to produce gari and to lesser extent fufu, lafun, animal feeds and other industrial purposes such as adhesives, etc 26.

    The cassava mill effluents generated during cassava processing in Nigeria are discharged into the environment with little or no treatment. Studies have shown that the effluents have some level of toxicity on the environment 2, 3, 14, 15, 16, 17, 18, 19, 20, 21 and its associated biota including some domestic animals, plants, fisheries, etc 2. Several biotechnological advances have shown the various means through which the effluents can be effectively managed 31. Among the various methods is the production of Saccharomyces cerevisiae biomass from the effluents 13. Studies have also indicated that when cassava mill effluents are treated some of its physicochemical and heavy metals characteristics are improved upon 14, 15. Therefore, this study aimed at assessing the fermentation dynamics of the Saccharomyces cerevisiae with regard to the microbial density.

    Materials and Methods

    Source of Cassava Mill Effluents

    Raw cassava mill effluents used in this study were obtained from a smallholder cassava processor in triplicate at Ndemili, Delta state, Nigeria. The samples were transported to the laboratory using ice pack and it was used immediately.

    Identification of Saccharomyces Cerevisae

    The Saccharomyces cerevisiae used for the study was isolated from palm wine purchased from a palm wine vendor in Rumuomasi, Port Harcourt, Nigeria. The palm wine was plated following pour plate microbial technique of Benson 32, Pepper and Gerba 33 on a potato dextrose agar containing chloramphenicol. The growths on the agar plate after incubation at room temperature were further streaked in another potato dextrose agar containing chloramphenicol. The resultant isolates was identified using standard microbiological protocol i.e. cultural, morphological, and physiological/biochemical characteristics including carbon fermentation and assimilation, glucose-peptone-yeast extract broth, growth based on temperature 34, 35, 36, staining process using lacto-phenol cotton blue stain and methylene blue indicators 14 and phenotypic characteristics 34, 35, 37. The resultant characteristics were compared with the scheme provided by Ellis et al. 37, Kurtzman and Fell 34, Iwuagwu and Ugwuanyi 35.

    Effluents Preparation

    The cassava mill effluents used for the study has pH of 3.93 at day 0 which moved toward alkalinity at day 5 (pH of 4.93), day 10 (pH of 5.33) and day 15 (pH of 6.30) 14. Similarly the temperature was 27.67 ºC, 27.57 ºC, 27.60 ºC and 27.20 ºC at day 0, 5, 10 and 15 respectively 14. The effluents was filtered with muslin cloth and then boiled. The boiled effluents were allowed to cool under aseptic condition and then after, 100ml of the effluents were dispensed into conical flask and 10ml of Saccharomyces cerevisiae inoculum 14, 15 with population of 2.43 x 106cfu/ml added. The conical flask was covered cotton wool wrapped with aluminum foil paper. The flasks containing the medium were intermittently shaked between 7.00 – 19.00 throughout the study 14, 15. At every 3 day of the 15 days study period (thus growth determination period was 0, 3,6, 9, 12 and 15 days) 1ml of the effluent were pipetted and plated in potato dextrose agar containing chloramphenicol and incubated for 3-5 days. The resultant Saccharomyces cerevisiae density was expressed as coliform forming using per ml (cfu/ml).

    Statistical Analysis

    SPSS version 20 was used for the statistical analysis. One way and analysis of variance carried out and p=0.05 and Waller-Duncan test statistics used to compare means values of the different days. The chart showing mean and standard deviation values were plotted using Paleontological statistics software package by Hammer et al. 38.

    Results and Discussion

    The growth pattern of Saccharomyces cerevisiae in cassava mill effluents is presented in Figure 1. At the initial day (day 0) the population of Saccharomyces cerevisiae were 0.00 x 106 cfu/ml, which rose to 2.88 x 106 cfu/ml at day 3, 14.50 x 106 cfu/ml at day 6, 23.0 x 106 cfu/ml at day 9, 272.67 x 106 cfu/ml at day 12 and decline slightly at day 15 (13.57 x 106 cfu/ml). Typically, there was significant variations (P<0.05) among the various period of study. But Waller-Duncan statistics showed that day 12 had the highest density (272.67 x 106 cfu/ml) which was significantly different among other days of study. This growth pattern showed that most of the nutrient in the cassava mill effluents has been utilized. Previous studies have indicated that Saccharomyces cerevisiae improves the physical and chemical characteristics of cassava mill effluents 14, 15. Izah et al. 14 reported a decline in total dissolved solid, conductivity, salinity, sulphate, phosphate, chemical oxygen demand, and increase in turbidity during treatment of cassava mill effluents with Saccharomyces cerevisiae. Izah et al. 15 also reported that Saccharomyces cerevisiae has the tendency to remove heavy metals (such as iron, zinc, copper, manganese) from cassava mill effluent through biosorption. Furthermore, Iwuagwu and Ugwuanyi 35 also reported that Saccharomyces cerevisiae could reduce the chemical oxygen demand and total dissolved solid concentration in palm oil mill effluents. Abioye et al. 39 reported that Saccharomyces cerevisiae aid in the degradation of pharmaceutical effluents. Okoduwa et al. 36 also reported the potentials of Saccharomyces cerevisiae in the treatment of tannery effluents. The nutrient uptake from the effluents suggests the possible increase in population of the Saccharomyces cerevisiae. A slight significant decline at day 15 suggest that most nutrients that are useful for the growth of Saccharomyces cerevisiae have been used up and therefore the cells requires addition nutrient for growth.

    Figure 1. Growth pattern of Saccharomyces cerevisiae in cassava mill effluents
     Growth pattern of Saccharomyces cerevisiae in cassava mill effluents


    This study showed that during the treatment of cassava mill effluents using Saccharomyces cerevisiae the microbial cells increased from day 1 to 12 and then significantly declined at day 15. This suggest that at 12 days of fermentation of cassava mill effluents most of the nutrients that could enhance microbial growth have been used up and/ or the medium becomes toxic to the Saccharomyces cerevisiae cells. Therefore, there is the need for further study to focus on the implication of changing Saccharomyces cerevisiae population during the treatment of cassava mill effluents so as to prevent the attendant impacts associated with the untreated effluents in the environment particularly soil.


    1.S C Izah. (2018) Estimation of Potential Cassava Mill Effluents Discharged into Nigerian Environment.Environmental Analysis and Ecology studies;2(5),EAES.000550.2018.
    2.S C Izah, S E Bassey, E I Ohimain. (2018) . Impacts of Cassava mill effleunts in Nigeria.Journal of Plant and Animal Ecology;1(1),14-42 .
    3.S C Izah, S E Bassey, E I Ohimain. (2018) Ecological risk assessment of heavy metals in cassava mill effluents contaminated soil in a rural community in the Niger Delta Region of Nigeria.Molecular Soil Biology,9(1),1-11.
    4.O A Aghoghovwia, F A Miri, S C Izah. (2018) . Impacts of Anthropogenic Activities on Heavy Metal Levels in Surface Water of Nun River around Gbarantoru and Tombia Towns, Bayelsa State, Nigeria.Annals of Ecology and Environmental Science,2(2) 1-8.
    5.O A Aghoghovwia, S C Izah, F A Miri. (2018) Environmental risk assessment of heavy metals in sediment of Nun River around Gbarantoru and Tombia Towns. , Bayelsa, State, Nigeria.Biological Evidence 8(3), 21-31.
    6.S C Izah, C N Angaye, A O, J O Nduka. (2017) Uncontrolled bush burning in the Niger Delta region of Nigeria: potential causes and impacts on biodiversity.International. , Journal of Molecular Ecology and Conservation 7(1), 1-15.
    7.S C Izah, E I Seiyaboh. (2018) Challenges of wildlife with therapeutic properties in Nigeria; a conservation perspective.International. , Journal of Avian & Wildlife Biology 3(4), 259-264.
    8.S C Izah, E I Seiyaboh. (2018) Changes in the protected areas of Bayelsa state. , Nigeria.International Journal of Molecular Evolution and Biodiversity 8(1), 1-11.
    9.S C Izah. (2018) Ecosystem of the Niger Delta region of Nigeria:. , Potentials and Threats.Biodiversity International Journal 2(4), 338-345.
    10.S C Izah, Angaye T C N. (2016) Heavy metal concentration in fishes from surface water in Nigeria: Potential sources of pollutants and mitigation measures.Sky. , Journal of Biochemistry Research,5(4),31-47
    11.S C Izah, Angaye T C N, E I Ohimain. (2016) Environmental Impacts of Oil palm processing in Nigeria.Biotechnological Research,2(3),132-141.
    12.E I Ohimain, S C Izah. (2013) Water minimization and optimization by small-scale palm oil mill in Niger Delta. , Nigeria.Journal of Water Research 135, 190-198.
    13.Izah S C. (2018) Estimation of Saccharomyces cerevisiae Biomass Cultured in Cassava Mill Effluents.Environmental Analysis and Ecology studies,2(5).EAES.000547.
    14.S C Izah, S E Bassey, E I Ohimain. (2017) Changes in the treatment of some physico-chemical properties of cassava mill effluents using Saccharomyces. 5(4), 28.
    15.S C Izah, S E Bassey, E I Ohimain. (2017) . Removal of Heavy Metals in Cassava Mill Effluents withSaccharomyces cerevisiaeisolated from Palm Wine.MOJ Toxicology,3(4) 00057.
    16.S C Izah, S E Bassey, E I Ohimain. (2017) Assessment of heavy metal in cassava mill effluent contaminated soil in a rural community in the Niger Delta region of Nigeria. , EC Pharmacology and Toxicology 4(5), 186-201.
    17.S C Izah, S E Bassey, E I Ohimain. (2017) Geo-accumulation index, enrichment factor and quantification of contamination of heavy metals in soil receiving cassava mill effluents in a rural community in the Niger Delta region of Nigeria.Molecular Soil. , Biology 8(2), 7-20.
    18.S C Izah, S E Bassey, E I Ohimain. (2017) Assessment of pollution load indices of heavy metals in cassava mill effluents contaminated soil: a case study of small-scale cassava processing mills in a rural community of the Niger Delta region of Nigeria.Bioscience Methods. 8(1), 1-17.
    19.S C Izah, S E Bassey, E I Ohimain. (2017) Assessment of Some Selected Heavy Metals. in Saccharomyces cerevisiae Biomass Produced from Cassava Mill Effluents.EC Microbiology 12(5), 213-223.
    20.S C Izah, S E Bassey, E I Ohimain. (2017) . Cyanide and Macro-Nutrients Content ofSaccharomyces cerevisiaeBiomass Cultured in Cassava Mill Effluents.International Journal of Microbiology and Biotechnology,2(4) 176-180.
    21.S C Izah, S E Bassey, E I Ohimain. (2017) Amino acid and proximate composition ofSaccharomyces cerevisiaebiomass cultivated in Cassava mill effluents.Molecular Microbiology Research,7(3),20-29.
    22.E I Seiyaboh, S C Izah. (2017) Review of Impact of Anthropogenic Activities in Surface Water Resources in the Niger Delta region of Nigeria: A case of Bayelsa state.International. , Journal of Ecotoxicology andEcobiology 2(2), 61-73.
    23.V N Ben-Eledo, L T Kigigha, S C Izah, B O Eledo. (2017) Water quality assessment of Epie creek in Yenagoa metropolis, Bayelsa state. , Nigeria.Archives of Current Research International 8-2;1-24.
    24.V N Ben-Eledo, L T Kigigha, S C Izah, B O Eledo. (2017) . , Bacteriological Quality Assessment of Epie Creek, Niger Delta Region of Nigeria.International Journal of Ecotoxicology andEcobiology 2(3), 102-108.
    25.E I Seiyaboh, S C Izah. (2017) Bacteriological assessment of a tidal creek receiving slaughterhouse wastes in Bayelsa state. , Nigeria.Journal of Advances in Biology and Biotechnology 14(1), 1-7.
    26.E I Seiyaboh, S C Izah. (2018) Mortality rate of juvenile Heterobranchus bidorsalis exposed to cassava mill effluents.Annals of Review and Research. In press
    27.S C Izah, Aigberua A O. (2017) Assessment of Microbial Quality of Cassava Mill Effluents Contaminated Soil in a Rural Community in the Niger Delta. , Nigeria.EC Microbiology 13(4), 132-140.
    28.S C Izah, E I Ohimain. (2015) Bioethanol production from cassava mill effluents supplemented with solid agricultural residues using bakers’ yeast [Saccharomyces cerevisiae].Journal of. , Environmental Treatment Techniques,3(1), 47 – 54.
    29.L T Kigigha, Nyenke P, S C Izah. (2018) Health risk assessment of selected heavy metals ingari(cassava flake) sold in some major markets in Yenagoa metropolis. , Nigeria.MOJToxicol 4(2), 47-52.
    30.E I Ohimain, D I Silas-Olu, J T Zipamoh. (2013) Biowastes generation by small scale cassava processing centres in Wilberforce Island, Bayelsa State. , Nigeria.Greener Journal of Environmental Management and Public safety 2(1), 51-59.
    31.S C Izah. (2018) Biovalue potentials of cassava mill effluents; an option for preventing the toxicological impacts of the wastewater on the ecosystems and its resources. In 7thInternational conference West African society of toxicology. Theme: Toxicological concerns in developing nations. Held in University of Abuja, Nigeria between 3rdand 6thofJuly 2018. .
    32.H J Benson.. (2002)Microbiological Applications:Laboratory Manual in General Microbiology/complete version, 5th edition.McGaraw-Hill NewYork .
    33.I L Pepper, C P Gerba. (2004) . Environmental microbiology. A laboratory manual. Second edition.Elsevieracademicpress
    34.Kurtzman C P.. Fell JW (1998)The Yeasts: A Taxonomic Study. 4th edition, Elsevier Science , Amsterdam, The Netherlands .
    35.J O Iwuagwu, J O Ugwuanyi. (2014) Treatment and Valorization of Palm Oil Mill Effluent through Production of Food Grade Yeast Biomass.Journal of Waste Management,
    36.Okoduwa S I R, Igiri B, C B Udeh, Edenta C, Gauje B. (2017) Tannery Effluent Treatment by Yeast Species Isolates from Watermelon. doi: 10.3390/toxics5010006.Toxics,5, 6;.
    37.Ellis D, Davis S, Alexiou H, Handke R, Bartley.R (2007)Descriptions of Medical Fungi.Second Edition. Printed in Adelaide by Nexus Print Solutions, Underdale. , South Australia
    38.Hammer Ø, Harper D A T, P D Ryan. (2001) PAST: Paleontological statistics software package for education and data analysis. , Palaeontologia Electronica 4(1), 9.
    39.O P Abioye, E O Afolayan, S A Aransiola. (2015) . Treatment of Pharmaceutical Effluent by Saccharomyces cerevisiae and Torulaspora delbrueckii Isolated from Spoilt Water Melon. DOI: 10.3923/rjet.2015.Research Journal of Environmental Toxicology .

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