Research Topic · Peer-Reviewed

Protein Purification

Protein purification is the set of techniques used to isolate a specific protein in homogeneous form from a complex biological mixture, a prerequisite for studying its structure, activity, and interactions and for producing it for analytical, industrial, and therapeutic use. Purification schemes exploit differences …

Curated from this journal's research 📚 12 peer-reviewed articles cited Cited 57× across the literature 🔖 ISSN 2377-2549

Overview

Protein purification is the set of techniques used to isolate a specific protein in homogeneous form from a complex biological mixture, a prerequisite for studying its structure, activity, and interactions and for producing it for analytical, industrial, and therapeutic use. Purification schemes exploit differences in physicochemical properties—size, charge, hydrophobicity, solubility, and binding specificity—through methods such as ammonium sulfate precipitation, ion-exchange, size-exclusion, hydrophobic-interaction, and affinity chromatography, often guided by affinity tags. Strategies typically proceed in stages, balancing yield against purity, and are monitored by electrophoresis, activity assays, and mass spectrometry, with affinity purification coupled to tandem mass spectrometry enabling both isolation and identification. Successful purification supports enzyme characterization, structural determination, and the production of recombinant and peptide-based products. The research reflected here includes affinity purification coupled with tandem mass spectrometry in mammalian cells, isolation of a human monoclonal single-chain antibody fragment, production and purification of microbial enzymes such as laccase, peroxidase, and cellulases, and characterization of purified oxidoreductases, alongside solid-phase peptide synthesis and chromatographic and mass-spectrometric analytical methods. The journal publishes peer-reviewed studies on protein and enzyme purification, biochemical characterization, and the analytical methods that support them.

Research published in this journal

12 peer-reviewed articles, ranked by relevance. Each links to its DOI.

How this research is being cited

The 12 articles above have been cited 57 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Nov 2026.

A sample of recent works citing this journal's research on Protein Purification, linking to each citing work.

Editorial oversight

Curated from peer-reviewed research published in Journal of New Developments in Chemistry (ISSN 2377-2549).

Journal editorial board
Zhe-Sheng Chen · United States Abel Jose de Sousa Costa Vieira · Portugal Amirhassan Amiri · Iran, Islamic Republic of

This page summarises published research for orientation; it is not medical or professional advice.