Overview
Methylene blue is a synthetic heterocyclic aromatic compound of the phenothiazine class, a cationic thiazine dye recognizable by its intense blue colour in the oxidized state and reversible reduction to the colourless leucomethylene blue. Its planar conjugated ring system and redox-active nitrogen and sulphur centres allow it to act as an electron acceptor and donor, which underpins its long-standing roles as a biological stain in histology and microbiology, a redox indicator, and a photosensitizer capable of generating reactive oxygen species under illumination. In analytical and physical chemistry it serves as a model adsorbate for evaluating the surface area, porosity, and uptake capacity of activated carbons, clays, and engineered nanomaterials, and as a probe in spectrophotometric and electrochemical methods. Pharmacologically, methylene blue is recognised for its capacity to reduce methemoglobin to functional hemoglobin and for its inhibition of nitric oxide synthase and guanylate cyclase. Characterisation of dye behaviour commonly draws on vibrational spectroscopy, electronic absorption, and quantum-chemical descriptors such as frontier molecular orbital energies and hyperpolarizability. These techniques connect the study of methylene blue to wider work on molecular structure, spectral fingerprinting, and material adsorption that defines contemporary chemical research.
Research published in this journal
12 peer-reviewed articles, ranked by relevance. Each links to its DOI.
Growing Threat Increased Carbapenem-Resistance among Klebsiella pneumoniae; Antibiotic Susceptibility Pattern of Klebsiella pneumoniae in a Tertiary Care Hospital
Morpho-biochemical Identification and Antimicrobial Susceptibility Testing of Bacterial Isolates from Chicken Eggs in District Faisalabad
Vibrational Spectral Analysis and First Order Hyperpolarizability Calculations on (E)-N′-(furan-2-yl methylene) Nicotinohydrazide
Delayed Laparoscopic Repair of Bladder Injury with Vesico Cutaneous Fistula in Fracture Pelvis
Tetrodotoxin (TTX) Time–Resolved Absorption and Resonance FT–IR and Raman Biospectroscopy and Density Functional Theory (DFT) Investigation of Vibronic–Mode Coupling Structure in Vibrational Spectra Analysis
FT-IR, FT-Raman, Homo-Lumo and UV-Visible Spectral Analysis of E-(N′-(1H-INDOL-3YL) Methylene Isonicotinohydrazide)
Growth Pattern of Saccharomyces cerevisiae in Cassava Mill Effluents
Performance Assessment of Some Operational Aspects of Revised National Tuberculosis Control Programme in Tuberculosis Unit, Nagpur, India
Aerobic Plate Count of Contaminants and Molecular Characterization of Eschereichia Coli in Raw Chicken Meat in Ismailia, Egypt
Oxidative Status and Reproductive Characteristics in Female Cavies (Cavia porcellus L.) Fed on Aqueous Extract of Avocado (Persea americana Mill.) Seed
Detection of carbapenem resistance mechanisms among Avian Pathogenic Escherichia coli (APEC) isolated from broiler chickens
How this research is being cited
The 12 articles above have been cited 176 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
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2026 · Computational and Theoretical Chemistry
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Ucop Haroen et al. · 2025 · Journal of Advanced Veterinary and Animal Research
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P. S. Singh et al. · 2025 · Journal of Molecular Structure
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B. Revanna et al. · 2025 · Journal of Molecular Structure
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2025 · Foods
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2025 · Foods
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2025 · Journal of Molecular Structure
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2025 · Journal of Molecular Structure
A sample of recent works citing this journal's research on Methylene Blue, linking to each citing work.