Bacterial Disk Diffusion

The bacterial disk diffusion test is a simple technique used to measure the antimicrobial susceptibility of bacteria. It involves the diffusion of antimicrobial agents contained in paper disks on the surface of an agar plate inoculated with a bacterial culture. The rate of diffusion of the antimicrobial agents is dependent on the concentration of the agents as well as the susceptibility of the bacterial culture. The end result is a clear zone around the disk where bacteria growth is inhibited, which can then be used to identify the susceptibility of the bacterial culture to the antimicrobial contained in the disk. The bacterial disk diffusion test is a simple and cost-effective way of measuring the antimicrobial susceptibility of a bacterial culture and can be used to select appropriate antibiotics for the treatment of infections.