Stable Isotope Labeling by Amino Acids in Cell Culture
(SILAC) Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) is a labeling technique used in proteomics to track proteins in cells and tissues. SILAC involves the incorporation of heavy isotope-labeled amino acids into proteins, allowing them to be detected, quantified and tracked in mass spectrometry experiments. This technique provides an accurate and quantitative way to measure changes in protein expression, making it an essential tool in many areas of biomedical research including cancer, drug discovery and the study of signal transduction pathways. This method of analysis is rapid and efficient, allowing scientists to gain a better understanding of the complexity of the cell and to answer important biological questions.
← International Journal of Cell