International Journal of Nutrition
ISSN: 2379-7835
Current Issue
Volume No: 3 Issue No: 1
share this page

Research Article | Open Access
  • Available online freely | Peer Reviewed
  • Consciousness Energy Healing Treatment: Impact on the Physicochemical and Thermal Characteristics of Folic Acid

    Gopal Nayak 1     Mahendra Kumar Trivedi 1     Alice Branton 1     Dahryn Trivedi 1     Snehasis Jana 2      

    1Trivedi Global, Inc., Henderson, USA.

    2Trivedi Science Research Laboratory Pvt. Ltd.,Bhopal, India.

    Abstract

    Folic acid is essential in the body for the production of DNA and also plays various vital functions within the body. This study on folic acid was done to analyze the results of the Trivedi Effect® on its physicochemical and thermal properties with the help of analytical techniques. The method involves dividing the test sample into two parts. The first part was termed as a control sample, and no treatment was given to it; while the second part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak and termed as the treated sample. The study revealed that the particle sizes reduced by 2.86%(d10), 6.34%(d50), 17.22%(d90), and 11.30%{D(4,3)}, thus increased the surface area by 1.72%, in the treated sample compared with the control sample. The PXRD data showed changes in the peak intensities and crystallite sizes ranging from -36.81% to 113.41% and -15.79% to 318%, respectively, thus increased in the average crystallite size by 22.55% of the treated sample compared with the control sample. The TGA analysis of the treated sample revealed a 3.94% increase in total weight loss that resulted in the remarkable reduction in the residual amount by 14.99%, in comparison to the untreated sample. The latent heat of fusion (ΔHfusion) and latent heat of decomposition (ΔHdegradation) were significantly altered by -10.33% and 10.46%, respectively in the treated sample compared to the untreated sample. The study denoted that the Biofield Energy Treatment is a novel approach that could be used to develop some polymorph of folic acid and that might improve its solubility, dissolution, and bioavailability in comparison to the control sample. Hence, the treated folic acid in the nutraceutical/pharmaceutical formulations might be useful concerning better drug performance and efficacy.

    Received 11 Oct 2018; Accepted 24 Oct 2018; Published 25 Oct 2018;

    Academic Editor:Jie Yin, Institute of Subtropical Agriculture & University of Chinese Academy of Sciences, China.

    Checked for plagiarism: Yes

    Review by: Single-blind

    Copyright©  2018 Gopal Nayak, et al.

    License
    Creative Commons License    This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Competing interests

    The authors have declared that no competing interests exist.

    Citation:

    Gopal Nayak, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Snehasis Jana (2018) Consciousness Energy Healing Treatment: Impact on the Physicochemical and Thermal Characteristics of Folic Acid. International Journal of Nutrition - 3(1):30-42.
    Download as RIS, BibTeX, Text (Include abstract )
    DOI10.14302/issn.2379-7835.ijn-18-2419

    Introduction

    Folic acid (vitamin B9) is the name used for a conjugate of 4-aminobenzoic acid and l-glutamic acid i.e., folate 1. Its biologically active form is tetrahydrofolic acid that acts similar to the cobalamins. Folic acid is important as it helps in the production of deoxyribonucleic acid (DNA) and also plays various vital functions within the body. The folic acid is synthetic form, while its natural form is folate that is found in food. It is generally used in combination with other B vitamins 2. The important food sources containing folate are cereals, leafy vegetables such as broccoli, spinach, lettuce, etc.; fruits such as melons, bananas, lemons, etc.; okra, legumes, asparagus, yeast, organ meat, mushrooms, and orange and tomato juice 3, 4.

    Folic acid is mainly used in the treatment and prevention of folate deficiency and the other associated complications, such as anaemia and malabsorption due to bowel inability 5. The use of folic acid is also evident in the prevention of stroke and heart disease, due to its property to reduce homocysteine level in the blood, which might create risk for heart disease. The other uses of folic acid involve the treatment of liver disease, ulcerative colitis, alcoholism, and kidney diseases such as dialysis. It is also used by pregnant women to prevent miscarriage and neural tube defects, such as spina bifida. Some researchers also indicated the use of folic acid in the prevention of colon and cervical cancer. Moreover, folic acid is used for the prevention and treatment of Alzheimer’s disease, memory loss, eye disease age-related macular degeneration, age-related hearing loss, aging, sleep problems, osteoporosis, depression, restless leg syndrome, nerve pain, vitiligo, gum infections, muscle pain, and Fragile-X syndrome, which is an inherited disease 6, 7, 8, 9. Folic acid also helps in reducing the side effects of lometrexol and methotrexate drugs and associated problems 10.

    The physicochemical properties of the drug are important regarding its absorption, distribution, metabolism, and excretion (ADME) profile and thus, it is advised to improve the biological activities and efficacy of the drug by altering its physicochemical properties 11. Consciousness Energy Healing Treatment is one among such approaches that could be used in modifying the physical, structural, and thermal properties of the drugs 12, 13. The Biofield Energy Healing is widely accepted as an alternative integrative approach that helps in improving the quality of life by correcting the root cause of the diseases 14, 15, 16. A human can harness energy from the universe and can transmit it to any living organism(s) or non-living object(s) around the globe. The object or recipient always receives the energy and responds in a useful way. This process is known as the Trivedi Effect®-Biofield Energy Healing Treatment 17, 18. The Trivedi Effect®- Consciousness Energy Healing Treatment has shown its effect on the crops and its productivity, microbes, metals, chemicals, ceramics, nutraceuticals, skin health, bioavailability, bone health, cancer 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, etc. This study was also designed to establish the physicochemical and thermal properties of the treated folic acid and to determine the impact of the Trivedi Effect® on the properties of folic acid as compared to the control one with the help of various analytical techniques.

    Materials and Methods

    Chemicals and Reagents

    Folic acid was purchased from Alfa Aesar, USA; whereas the remaining chemicals used in the experiments were of analytical grade purchased from India.

    Consciousness Energy Healing Treatment Strategies

    The folic acid sample used in the study was first divided into two parts. The first part of the samples was not given the Biofield Energy Treatment and considered as the control sample. Besides, the second part of the sample was received the Trivedi Effect®-Energy of Consciousness Healing Treatment under standard laboratory conditions for 3 minutes and known as the Biofield Energy Treated folic acid sample. This Biofield Energy Treatment was provided remotely through the healer’s unique energy transmission process by a famous Biofield Energy Healer, Gopal Nayak, India, to the test sample. Later on, the control sample was treated with a “sham” healer for comparison purpose. The “sham” healer did not have any knowledge about the Biofield Energy Treatment. Now, the control and Biofield Energy Treated sample were kept in sealed conditions and characterized using modern analytical techniques.

    Characterization

    Particle Size Analysis (PSA)

    The particle size analysis of folic acid samples was carried out on Malvern Mastersizer 2000, from the UK with a detection range between 0.01 µm to 3000 µm using wet method 38, 39. The % change in particle size (d) at below 10% level (d10), 50% level (d50), 90% level (d90),and D(4,3) was calculated using the following equation 1:

    (1)

    Where dControland dTreatedare the particle size (μm) of the control and the Biofield Energy Treated folic acid samples, respectively.

    The % change in surface area (S) was calculated using following equation 2:

    (2)

    Where SControl and STreated are the surface area of the control and treated folic acid samples, respectively.

    Powder X-ray Diffraction (PXRD) Analysis

    The PXRD analysis of control and the Biofield Energy Treated folic acid was performed with the help of Rigaku MiniFlex-II Desktop X-ray diffractometer (Japan) 40, 41. The average size of individual crystallites was calculated from XRD data using the Scherrer’s formula 3:

    G = kλ/βcosθ(3)

    Where k is the equipment constant (0.94), G is the crystallite size in nm, λ is the radiation wavelength (0.154056 nm for Kα1 emission), β is the full-width at half maximum (FWHM), and θ is the Bragg angle 42.

    The % change in crystallite size (G) of folic acid was calculated using the following equation 4:

    (4)

    Where GControl and GTreated are the crystallite size of the control and treated samples, respectively.

    Thermal Gravimetric Analysis (TGA)/ Differential thermogravimetric analysis (DTG)

    TGA/DTG thermograms of the control and the Biofield Energy Treated folic acid were obtained with the help of TGA Q50TA instruments. A sample of 4-15 mg was loaded to the platinum crucible at a heating rate of 10ºC/min from 25°C to 1000°C with the recent literature 43. The % change in weight loss (W) was calculated using the following equation 5:

    (5)

    Where WControl and WTreated are the weight loss of the control and the Biofield Energy Treated folic acid, respectively.

    The % change in maximum thermal degradation temperature (Tmax) (M) was calculated using the following equation 6:

    (6)

    Where MControl and MTreated are the Tmax values of the control and treated folic acid, respectively.

    Differential Scanning Calorimetry (DSC)

    The DSC analysis of folic acid was performed with the help of DSC Q200, TA instruments. A sample of ~1-5 mg was loaded to the aluminium sample pan at a heating rate of 10ºC/min from 30°C to 350°C 43. The % change in melting point (T) was calculated using the following equation 7:

    (7)

    Where TControl and TTreated are the melting point of the control and treated samples, respectively.

    The % change in the latent heat of fusion (ΔH) was calculated using the following equation 8:

    (8)

    Where ΔHControland ΔHTreatedare the latent heat of fusion of the control and treated folic acid, respectively.

    Statistical Analysis

    The values were represented as Mean ± SEM (standard error of mean) of the independent experiments. For two groups comparison student’s t-test was used. Statistically significant values were set at the level of p≤0.05.

    Results and Discussion

    Particle Size Analysis (PSA)

    The particle size analysis corresponding to d10, d50, d90, and D(4, 3) of the control and the Biofield Energy Treated sample was done and the results are mentioned in Table 1. It revealed that the particle size distribution of the treated folic acid sample was significantly reduced by 7.01%, 6.53%, 8.37%, and 15.99% at d10, d50, d90, and D(4, 3), respectively, compared to the control sample.

    Table 1. Particle size distribution of the control and the Biofield Energy Treated folic acid.
    Parameter d 10 (µm) d 50 (µm) d 90 (µm) D( 4,3) (µm) SSA(m 2 /g)
    Control 1.71 4.75 17.81 8.69 1.74
    Biofield Treated 1.59 4.44 16.32 7.30 1.77
    Percent change* (%) -7.01 -6.53 -8.37 -15.99 1.72

    * d10, d50, and d90: particle diameter corresponding to 10%, 50%, and 90% of the cumulative distribution, D(4,3): the average mass-volume diameter, and SSA: the specific surface area. denotes the percentage change in the Particle size distribution of the Biofield Energy Treated sample with respect to the control sample.

    The decrease in particle size resulted in the increase in specific surface area (SSA) of the treated sample as the SSA was observed as 1.74 m2/g in the control sample; while it was increased by 1.72% and observed as 1.77 m2/g in the treated sample. The particle size distribution is important regarding the efficacy and performance of drug within the body in terms of its solubility, dissolution, and bioavailability 11, 44. The decreased particle size of the treated folic acid that resulted in the increased surface area could be used as an approach to improve the bioavailability and efficacy of drug 45. Hence, it could be anticipated that the treated folic acid might show better bioavailability profile after the Biofield Energy Treatment when used in the pharmaceutical and nutraceutical formulations in comparison to the untreated sample.

    Powder X-ray Diffraction (PXRD) Analysis

    The PXRD diffractograms of the control and treated folic acid samples are shown in Figure 1. The analysis revealed the crystalline nature of both the samples as shown by the sharp and intense peaks present in their diffractograms. The further analysis was done (Table 2) to determine any changes in the Bragg’s angles, relative peak intensities, and crystallite sizes corresponding to the characteristic peaks of the treated sample as compared to the control sample.

    Figure 1. PXRD diffractograms of the control and the Biofield Energy Treated folic acid.
    Figure 1.

    Table 2. PXRD data for the control and the Biofield Energy Treated folic acid.
    Entry No. Bragg angle (°2 θ) Intensity (cps) Crystallite size (G, nm)
    Control Treated Control Treated % change * Control Treated % change *
    1 5.46 5.49 307 194 -36.81 99 240 142.42
    2 10.88 10.96 373 559 49.87 195 203 4.1
    3 12.29 13.07 261 557 113.41 50 209 318
    4 16.95 16.99 357 491 37.54 84 78.1 -7.02
    5 19.23 19.31 91 118 29.67 227 218 -3.96
    6 20.45 20.55 188 237 26.06 114 96 -15.79
    7 21.68 21.76 112 117 4.46 181 212 17.13
    8 26.66 26.69 593 870 46.71 110 110 0
    9 27.75 27.77 253 470 85.77 199 168 -15.58
    10 29.6 29.52 565 436 -22.83 42 92 119.05
    11 31.27 31.31 109 145 33.03 103 120 16.5
    12 34.34 34.46 111 146 31.53 91 86 -5.49
    13 Average crystallite size ± SEM 124.58 ± 17.57 152.68 ± 17.65 22.55

    * denotes the percentage change in the crystallite size of the Biofield Energy Treated sample with respect to the control sample,
    SEM: standard error of the mean.

    The results revealed that the Bragg’s angles of the peaks present in the diffractogram of the treated sample were slightly altered compared to the control sample; however the major alteration was observed in the peak at 2θ equals to 12.29º in the control sample that was observed at 2θ equals to 13.07º (entry no. 2) in the treated sample’s diffractogram. Besides, the peak intensities of the treated folic acid sample were altered ranging from -36.81% to 113.41%; and the crystallite sizes were varied ranging from -15.79% to 318%, compared to the control sample. The major alteration was also observed in the average crystallite size as it was observed as 152.68 nm in the treated sample, which was significantly increased by 22.55% in comparison to the control sample (124.58 nm). The alterations in the crystalline structure, as well as crystal morphology of drugs have been reported previously by using the Biofield Energy Treatment. It is supposed that the Consciousness Energy Healing Treatment might form a new polymorph of the compound by changing the peak intensities and crystallite sizes 46, 47. Such novel polymorph of folic acid might possess better bioavailability and drug profile compared with the untreated sample.

    Thermal Gravimetric Analysis (TGA)/ Differential Thermogravimetric Analysis (DTG)

    The analysis of the thermal stability of both the samples, i.e., the control and treated samples were done using TGA/DTG technique. The TGA thermograms of both the samples are presented in Figure 2 and the results (Table 3) revealed that the total weight loss of the treated folic acid during thermal degradation was increased by 3.94% as compared to the control sample. Therefore, the residual amount remaining after the degradation of the Biofield Energy Treated sample was observed to be significantly reduced by 14.99% in comparison to the control folic acid sample. Hence, it indicated the increased thermal degradation of the treated sample compared to the untreated folic acid sample.

    Figure 2. TGA thermograms of the control and the Biofield Energy Treated folic acid.
    Figure 2.

    Table 3. TGA/DTG data of the control and the Biofield Energy Treated samples of folic acid.
    Sample TGA DTG { Tmax (°C)}
    Total weight loss (%) Residue % Peak 1 Peak 2 Peak 3 Peak 4
    Control 79.19 20.81 107.71 247.93 433.78 709.61
    Biofield Energy Treated 82.31 17.69 109.04 249.63 446.32 699.77
    % Change* 3.94 -14.99 1.23 0.68 2.89 -1.39

    * denotes the percentage change of the Biofield Energy Treated sample with respect to the control sample,
    Tmax = the temperature at which maximum weight loss takes place in TG or peak temperature in DTG.

    The DTG analysis of both the samples showed four peaks in their respective thermograms (Figure 3). It was observed that the maximum thermal degradation temperatures (Tmax) in the treated sample corresponding to 1st, 2nd and 3rd peaks were increased by 1.23%, 0.68%, and 2.89%, respectively compared to the control sample. However, the treated sample showed a slight reduction in the Tmax of the 4th peak by 1.39% in comparison to the control folic acid sample. Thus, the overall analysis revealed improvement in the thermal degradation temperature of the Biofield Energy Treated sample compared with the control sample.

    Figure 3. DTG thermograms of the control and the Biofield Energy Treated folic acid.
    Figure 3.

    Differential Scanning Calorimetry (DSC) Analysis

    The DSC analysis was used in this study to determine the thermal behaviour of the samples such as melting, crystallization temperature, etc.48. According to the scientific literature, the heating of folic acid causes the breaking of the “Glu” moiety first at ~180°C, after which the degradation of pterin and PABA takes place in an overlapping form. Moreover, upon further heating, there was the loss of amide and acid functionalities of the compound at ~195 °C, and heating beyond that degrades the crystalline folic acid in the form of amorphous one above 200°C 49.

    There were two peaks in the DSC thermograms of the control and treated sample (Figure 4) and the further analysis related to peak temperature and enthalpy change for both the samples was presented in Table 4. The first peak i.e., endothermic in nature, was observed in both the thermograms that indicated the melting of the control and treated sample. The peak temperature for the treated sample was observed to be increased by 2.42%, along with 10.33% reduction in the latent heat of fusion (ΔHfusion) compared to the control sample (Table 4). Besides, the second peak in both the samples is broad and exothermic in nature and might indicate the degradation of the samples after further heating. The analysis showed that the degradation temperature of the treated sample was at 231.11°C, i.e., reduced by 1.60% in comparison to the control sample (234.86°C). Moreover, the latent heat of degradation (ΔHdegradation) corresponding to this peak of the treated folic acid sample was significantly increased by 10.46% in comparison to the control sample (Table 4).

    Figure 4. DSC thermograms of the control and the Biofield Energy Treated folic acid.
    Figure 4.

    Table 4. Comparison of DSC data between the control and the Biofield Energy Treated folic acid.
    Peak Description Melting Point (°C) ΔH (J/g)
    Peak 1 Control sample 184.82 113.30
    Biofield Treated sample 189.29 101.60
    % Change* 2.42 -10.33
    Peak 2 Control sample 234.86 54.85
    Biofield Treated sample 231.11 60.59
    % Change* -1.60 10.46

    ΔH : Latent heat of fusion/decomposition;
    * denotes the percentage change of the Biofield Energy Treated sample with respect to the control sample.

    The DSC results denoted that the thermal properties of treated folic acid were altered after the Biofield Energy Treatment that might occur due to some changes in the crystallization structure 50 of the treated folic acid as evident in the PXRD analysis. Hence, it is presumed that the Biofield Energy Treated folic acid might be more stable against heating compared to the untreated sample.

    Conclusions

    The study of Biofield Energy Treated folic acid revealed the significant effect of the Trived Effect®-Consciousness Energy Healing Treatment on its physicochemical and thermal properties. It indicated the significant changes in the particle size distribution of folic acid after the Biofield Energy Treatment. The treated sample showed reduced particle size at d10, d50, d90, and D(4,3) by 7.01%, 6.53%, 8.37%, and 15.99%, respectively compared to the control sample. The decrease in particle size of the Biofield Energy Treated sample causes an increase in the specific surface area by 1.72% compared to the control folic acid sample. The PXRD diffractograms indicated significant alterations in the peak intensities and crystallite sizes ranging from-36.81% to 113.41% and -15.79% to 318%, respectively, compared to the untreated sample. The average crystallite size of the treated folic acid sample was found to be increased by 22.55% after the Biofield Energy Treatment in comparison to the control sample. The TGA data showed the increase in total weight loss of the Biofield Energy Treated sample by 3.94% that resulted in significant reduction in residue weight by 14.99%, compared to the control sample. The treated sample showed that the melting temperature and ΔHfusion were altered by 2.42% and -10.33%, respectively; while the degradation temperature and ΔHdegradation were changed by -1.60% and 10.46%, respectively compared to the untreated folic acid sample. The overall data indicated that the Trivedi Effect®-Consciousness Energy Healing Treated folic acid formed a new polymorph that may improve the solubility, dissolution, absorption, and bioavailability along with thermal stability compared to the untreated sample. Thus, it could be presumed that the Biofield EnergyTreated folic acid would be more efficacious in the pharmaceutical/nutraceutical preparations regarding the prevention and treatment of several diseases such as Alzheimer’s disease, memory loss, eye disease, age-related macular degeneration, age-related hearing loss, aging, allergic diseases, sleep problems, osteoporosis, depression, restless leg syndrome, nerve pain, vitiligo, gum infections, muscle pain, Fragile-X syndrome, etc.

    Acknowledgements

    The authors are grateful to Central Leather Research Institute, SIPRA Lab. Ltd., Trivedi Science, Trivedi Global, Inc., Trivedi Testimonials, and Trivedi Master Wellness for their assistance and support during this work.

    References

    1.Guilland J C, Aimone-Gastin I. (2013) . Vitamin B9. Rev Prat 63(1079), 1081-1084.
    2.Carmel R. (2005) Folic Acid. In:. Modern Nutrition in Health and Disease.Shils M, Shike M, Ross A, Caballero B, Cousins R(Ed.). Lippincott Williams & Wilkins,Baltimore, MD .
    3.Pobocik R S, Richer J J. (2002) Estimated intake and food sources of vitamin A, folate, vitamin C, vitamin E, calcium, iron, and zinc for Guamanian children aged 9 to 12. Pac Health Dialog. 9, 193-202.
    4.Whittaker P, Tufaro P R, Rader J I. (2001) Iron and folate in fortified cereals. , J Am Coll Nutr 20, 247-254.
    5.Kelly G S. (1998) Folates: Supplemental forms and therapeutic applications. , Altern Med Rev 3, 208-220.
    6.Fenech M. (2012) Folate. (vitamin B9). and vitamin B12and their function in the maintenance of nuclear and mitochondrial genome integrity. Mutat Res. 733, 21-33.
    7.Greenberg J A, Bell S J, Guan Y, Yu Y. (2011) Folic Acid supplementation and pregnancy: More than just neural tube defect prevention. Rev Obstet Gynecol. 4, 52-59.
    8.Allen L H. (2008) Causes of vitamin B12and folate deficiency. , Food Nutr Bull 29, 20-34.
    9.Scholl T O, Johnson W G. (2000) Folic acid: influence on the outcome of pregnancy. , Am J Clin Nutr 71, 1295-1303.
    10.Harten P. (2005) Reducing toxicity of methotrexate with folic acid. , Z Rheumatol 64, 353-358.
    11.Khadka P, Ro J, Kim H, Kim I, Kim J T. (2014) Pharmaceutical particle technologies: An approach to improve drug solubility, dissolution and bioavailability. , Asian J Pharm 9, 304-316.
    12.Trivedi M K, Branton A, Trivedi D, Shettigar H, Bairwa K. (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res. 3, 186.
    13.Trivedi M K, Tallapragada R M, Branton A, Trivedi D, Nayak G. (2015) The potential impact of biofield energy treatment on the atomic and physical properties of antimony tin oxide nanopowder. , American Journal of Optics and Photonics 3, 123-128.
    14.Warber S L, Cornelio D, Straughn J, Kile G. (2004) Biofield energy healing from the inside. , J Altern Complement Med 10, 1107-1113.
    15.Hammerschlag R, Levin M, McCraty R, Bat N, Ives J A. (2015) Biofield Physiology: A Framework for an Emerging Discipline. Glob Adv Health Med. 4, 35-41.
    16.Koithan M. (2009) Introducing complementary and alternative therapies. , J Nurse Pract 5, 18-20.
    17.Trivedi M K, Tallapragada R M, Branton A, Trivedi D, Nayak G. (2015) Spectral and thermal properties of biofield energy treated cotton. , American Journal of Energy Engineering 3, 86-92.
    18.Trivedi M K, Patil S, Shettigar H, Bairwa K, Jana S. (2015) Effect of biofield treatment on spectral properties of paracetamol and piroxicam. , Chem Sci J 6, 98.
    19.Trivedi M K, Branton A, Trivedi D, Nayak G, Mondal S C. (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated alphonso mango. , (MangiferaindicaL.).. Journal of Food and Nutrition Sciences 3, 245-250.
    20.Trivedi M K, Branton A, Trivedi D, Nayak G, Mondal S C. (2015) Evaluation of biochemical marker – Glutathione and DNA fingerprinting of biofield energy treatedOryza sativa. , American Journal of BioScience 3, 243-248.
    21.Trivedi M K, Branton A, Trivedi D, Nayak G, Charan S. (2015) Phenotyping and 16S rDNA analysis after biofield treatment onCitrobacterbraakii: A urinary pathogen. , J Clin Med Genom 3, 129.
    22.Trivedi M K, Patil S, Shettigar H, Mondal S C, Jana S. (2015) Evaluation of biofield modality on viral load of Hepatitis B and C viruses. , J AntivirAntiretrovir 7, 083-088.
    23.Trivedi M K, Patil S, Shettigar H, Mondal S C, Jana S. (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB System. Mycobact Dis. 5, 189.
    24.Trivedi M K, Patil S, Shettigar H, Bairwa K, Jana S. (2015) Phenotypic and biotypic characterization ofKlebsiellaoxytoca: An impact of biofield treatment. , J MicrobBiochemTechnol 7, 203-206.
    25.Nayak G, Altekar N. (2015) Effect of biofield treatment on plant growth and adaptation. , J Environ Health Sci 1, 1-9.
    26.Trivedi M K, Tallapragada R M. (2008) A transcendental to changing metal powder characteristics. , Met Powder Rep 63, 22-28.
    27.Trivedi M K, Nayak G, Patil S, Tallapragada R M, Latiyal O. (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. IndEng Manage. 4, 161.
    28.Trivedi M K, Nayak G, Patil S, Tallapragada R M, Latiyal O. (2015) Effect of biofield energy treatment on physical and structural properties of calcium carbide and praseodymium oxide. , International Journal of Materials Science and Applications 4, 390-395.
    29.Trivedi M K, Branton A, Trivedi D, Nayak G, Singh R. (2015) Characterization of Physical, Spectral and Thermal Properties of Biofield treated Resorcinol. Organic Chem Curr Res. 4-146.
    30.Trivedi M K, Branton A, Trivedi D, Nayak G, Plikerd W D. (2017) A Systematic study of the biofield energy healing treatment on physicochemical, thermal, structural, and behavioral properties of magnesium gluconate. , International Journal of Bioorganic Chemistry 2, 135-145.
    31.Trivedi M K, Branton A, Trivedi D, Nayak G, Plikerd W D. (2017) Chromatographic and spectroscopic characterization of the consciousness energy healing treatedWithaniaSomnifera.(ashwagandha). root extract. , European Journal of Biophysics 5, 38-47.
    32.Kinney J P, Trivedi M K, Branton A, Trivedi D, Nayak G. (2017) Overall skin health potential of the biofield energy healing based herbomineral formulation using various skin parameters. , American Journal of Life Sciences 5, 65-74.
    33.Branton A, Jana S. (2017) The influence of energy of consciousness healing treatment on low bioavailable resveratrol in maleSprague Dawleyrats. , International Journal of Clinical and Developmental Anatomy 3, 9-15.
    34.Branton A, Jana S. (2017) The use of novel and unique biofield energy healing treatment for the improvement of poorly bioavailable compound, berberine in maleSprague Dawleyrats. , American Journal of Clinical and Experimental Medicine 5, 138-144.
    35.Branton A, Jana S. (2017) Effect of The biofield energy healing treatment on the pharmacokinetics of 25-hydroxyvitamin D3 [25 (OH) D3] in rats after a single oral dose of vitamin D3. , American Journal of Pharmacology and Phytotherapy 2, 11-18.
    36.Koster D A, Trivedi M K, Branton A, Trivedi D, Nayak G. (2018) Evaluation of biofield energy treated vitamin D3on bone health parameters in human bone osteosarcoma cells. (MG-63).. Biochemistry and Molecular Biology. 3, 6-14.
    37.Trivedi M K, Patil S, Shettigar H, Gangwar M, Jana S. (2015) vitroevaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. , J Cancer SciTher 7, 253-257.
    38.Trivedi M K, Sethi K K, Panda P, Jana S. (2017) Physicochemical, thermal and spectroscopic characterization of sodium selenate using XRD, PSD, DSC, TGA/DTG, UV-vis, and FT-IR. , Marmara Pharmaceutical Journal 21(2), 311-318.
    39.Trivedi M K, Sethi K K, Panda P, Jana S. (2017) A comprehensive physicochemical, thermal, and spectroscopic characterization of zinc. (II). chloride using Xray diffraction, particle size distribution, differential scanning calorimetry, thermogravimetric analysis/differential thermogravimetric analysis, ultravioletvisible, and Fourier transforminfrared spectroscopy. , International Journal of Pharmaceutical Investigation 7, 33-40.
    40.Zhang T, Paluch K, Scalabrino G, Frankish N, Healy A M. (2015) Molecular structure studies of (1S,2S).-2-benzyl-2,3-dihydro-2-. , (1Hinden-2-yl).-1H-inden-1-ol. J Mol Struct 1083, 286-299.
    41. (1997) Desktop X-ray Diffractometer “MiniFlex+”. , The Rigaku Journal 14, 29-36.
    42.Langford J I, AJC Wilson. (1978) Scherrer after sixty years: A survey and some new results in the determination of crystallite size. , J Appl Cryst 11, 102-113.
    43.Trivedi M K, Branton A, Trivedi D, Nayak G, Plikerd W D. (2017) A systematic study of the biofield energy healing treatment on physicochemical, thermal, structural, and behavioral properties of iron sulphate. , International Journal of Bioorganic Chemistry 2, 135-145.
    44.Loh Z H, Samanta A K, PWS Heng. (2015) Overview of milling techniques for improving the solubility of poorly water-soluble drugs. , Asian J Pharm 10, 255-274.
    45.Hu J, Johnston K P, Williams R O. (2004) Nanoparticle engineering processes for enhancing the dissolution rates of poorly water soluble drugs. Drug Dev Ind Pharm. 30, 233-245.
    46.Trivedi M K, Branton A, Trivedi D, Nayak G, Plikerd W D. (2017) Evaluation of the physicochemical, spectral, thermal and behavioral properties of sodium selenate: influence of the energy of consciousness healing treatment. , American Journal of Quantum Chemistry and Molecular Spectroscopy 2, 18-27.
    47.Trivedi M K, Branton A, Trivedi D, Nayak G, Lee A C. (2017) Evaluation of the impact of biofield energy healing treatment. (the Trivedi Effect®). on the physicochemical, thermal, structural, and behavioural properties of magnesium gluconate. , International Journal of Nutrition and Food Sciences 6, 71-82.
    48.Gill P, Moghadam T T, Ranjbar B. (2010) Differential Scanning Calorimetry Techniques: Applications in Biology and Nanoscience. , J Biomol Tech 21, 167-193.
    49.Gazzali A M, Lobry M, Colombeau L, Acherar S, Azaïs H. (2016) Stability of folic acid under several parameters. , Eur J Pharm Sci 93, 419-430.
    50.Zhao Z, Xie M, Li Y, Chen A, Li G. (2015) Formation of curcumin nanoparticlesviasolution enhanced dispersion by supercritical CO2. , Int J Nanomedicine 10, 3171-3181.