Consciousness Energy Healing Treatment: Impact on the Physicochemical and Thermal Characteristics of Folic Acid

Folic acid is essential in the body for the production of DNA and also plays various vital functions within the body. This study on folic acid was done to analyze the results of the Trivedi Effect on its physicochemical and thermal properties with the help of analytical techniques. The method involves dividing the test sample into two parts. The first part was termed as a control sample, and no treatment was given to it; while the second part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak and termed as the treated sample. The study revealed that the particle sizes reduced by 7.01% (d10), 6.53% (d50), 8.37% (d90), and 15.99% {D(4,3)}, thus increased the surface area by 1.72%, in the treated sample compared with the control sample. The PXRD data showed changes in the peak intensities and crystallite sizes ranging from -36.81% to 113.41% and -15.79% to 318%, respectively, thus increased in the average crystallite size by 22.55% of the treated sample compared with the control sample. The TGA analysis of the treated sample revealed a 3.94% increase in total weight loss that resulted in the remarkable reduction in the residual amount by 14.99%, in comparison to the untreated sample. The latent heat of fusion (ΔHfusion) and latent heat of decomposition (ΔHdegradation) were significantly altered by -10.33% and 10.46%, respectively in the treated sample compared to the untreated sample. The study denoted that the Biofield Energy Treatment is a novel approach that could be used to develop some polymorph of folic acid and that might improve its solubility, dissolution, and bioavailability in comparison to the control sample. Hence, the treated folic acid in the nutraceutical/pharmaceutical formulations might be useful concerning better drug performance and efficacy. DOI:10.14302/issn.2379-7835.ijn-18-2419 Correspondig author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India. Tel: +91-022-25811234; Email: publication@trivedieffect.com


Introduction
Folic acid (vitamin B 9 ) is the name used for a conjugate of 4-aminobenzoic acid and l-glutamic acid i.e., folate [1]. Its biologically active form is tetrahydrofolic acid that acts similar to the cobalamins.
Folic acid is important as it helps in the production of deoxyribonucleic acid (DNA) and also plays various vital functions within the body. The folic acid is synthetic form, while its natural form is folate that is found in food. It is generally used in combination with other B vitamins [2]. The important food sources containing folate are cereals, leafy vegetables such as broccoli, spinach, lettuce, etc.; fruits such as melons, bananas, lemons, etc.; okra, legumes, asparagus, yeast, organ meat, mushrooms, and orange and tomato juice [3,4].
Folic acid is mainly used in the treatment and prevention of folate deficiency and the other associated complications, such as anaemia and malabsorption due to bowel inability [5]. The use of folic acid is also evident in the prevention of stroke and heart disease, due to its property to reduce homocysteine level in the blood, which might create risk for heart disease. The other uses of folic acid involve the treatment of liver disease, ulcerative colitis, alcoholism, and kidney diseases such as dialysis. It is also used by pregnant women to prevent miscarriage and neural tube defects, such as spina bifida. Some researchers also indicated the use of folic acid in the prevention of colon and cervical cancer.
Moreover, folic acid is used for the prevention and treatment of Alzheimer's disease, memory loss, eye disease age-related macular degeneration, age-related hearing loss, aging, sleep problems, osteoporosis, depression, restless leg syndrome, nerve pain, vitiligo, gum infections, muscle pain, and Fragile-X syndrome, which is an inherited disease [6][7][8][9]. Folic acid also helps in reducing the side effects of lometrexol and methotrexate drugs and associated problems [10].
The physicochemical properties of the drug are important regarding its absorption, distribution, metabolism, and excretion (ADME) profile and thus, it is advised to improve the biological activities and efficacy of the drug by altering its physicochemical properties [11].
Consciousness Energy Healing Treatment is one among such approaches that could be used in modifying the physical, structural, and thermal properties of the drugs [12,13]. The Biofield Energy Healing is widely accepted as an alternative integrative approach that helps in improving the quality of life by correcting the root cause of the diseases [14][15][16]. A human can harness energy from the universe and can transmit it to any living organism(s) or non-living object(s) around the globe. The object or recipient always receives the energy and responds in a useful way. This process is known as the Trivedi Effect ® -Biofield Energy Healing Treatment [17,18]. The Trivedi Effect ® -Consciousness Energy Healing Treatment has shown its effect on the crops and its productivity, microbes, metals, chemicals, ceramics, nutraceuticals, skin health, bioavailability, bone health, cancer [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37], etc. This study was also designed to establish the physicochemical and thermal properties of the treated folic acid and to determine the impact of the Trivedi Effect ® on the properties of folic acid as compared to the control one with the help of various analytical techniques.

Chemicals and Reagents
Folic acid was purchased from Alfa Aesar, USA; whereas the remaining chemicals used in the experiments were of analytical grade purchased from India.

Consciousness Energy Healing Treatment Strategies
The folic acid sample used in the study was first divided into two parts.
Where S Control and S Treated are the surface area of the control and treated folic acid samples, respectively.

Powder X-ray Diffraction (PXRD) Analysis
The PXRD analysis of control and the Biofield Energy Treated folic acid was performed with the help of Rigaku MiniFlex-II Desktop X-ray diffractometer (Japan) [40,41]. The average size of individual crystallites was calculated from XRD data using the Scherrer's formula 3: Where k is the equipment constant (0.94), G is the crystallite size in nm, λ is the radiation wavelength Where T Control and T Treated are the melting point of the control and treated samples, respectively.
The % change in the latent heat of fusion (ΔH) was calculated using the following equation 8: Where ΔH Control and ΔH Treated are the latent heat of fusion of the control and treated folic acid, respectively.

Statistical Analysis
The values were represented as Mean ± SEM (standard error of mean) of the independent experiments. For two groups comparison student's t-

Results and Discussion
Particle Size Analysis (PSA) The particle size analysis corresponding to d 10  compared to the control sample.
The decrease in particle size resulted in the increase in specific surface area (SSA) of the treated sample as the SSA was observed as 1.74 m 2 /g in the control sample; while it was increased by 1.72% and observed as 1.77 m 2 /g in the treated sample. The particle size distribution is important regarding the efficacy and performance of drug within the body in terms of its solubility, dissolution, and bioavailability [11,44]. The decreased particle size of the treated folic acid that resulted in the increased surface area could be used as an approach to improve the bioavailability and efficacy of drug [45]. Hence, it could be anticipated that the

Differential Scanning Calorimetry (DSC) Analysis
The DSC analysis was used in this study to determine the thermal behaviour of the samples such as melting, crystallization temperature, etc. [48]. According There were two peaks in the DSC thermograms of the control and treated sample ( Figure 4) and the further analysis related to peak temperature and enthalpy change for both the samples was presented in Table 4. The first peak i.e., endothermic in nature, was observed in both the thermograms that indicated the melting of the control and treated sample. The peak temperature for the treated sample was observed to be increased by 2.42%, along with 10.33% reduction in the latent heat of fusion (ΔH fusion ) compared to the control sample (Table 4) (Table 4).
The DSC results denoted that the thermal properties of treated folic acid were altered after the Biofield Energy Treatment that might occur due to some changes in the crystallization structure [50] of the treated folic acid as evident in the PXRD analysis. Hence, it is presumed that the Biofield Energy Treated folic acid might be more stable against heating compared to the untreated sample.

Conclusions
The study of Biofield Energy Treated folic acid revealed the significant effect of the Trived Effect ® -Consciousness Energy Healing Treatment on its physicochemical and thermal properties. It indicated the significant changes in the particle size distribution of folic acid after the Biofield Energy Treatment. The treated sample showed reduced particle size at d 10     using X-ray diffraction, particle size distribution, differential scanning calorimetry, thermogravimetric analysis/differential thermogravimetric analysis, ultraviolet-visible, and Fourier transform-infrared