Serial Analysis of Gene Expression
Serial Analysis of Gene Expression (SAGE) is a high-throughput technique used to measure gene expression. It works by generating short sequences called 'tags’ which are unique, short DNA sequences that are associated with the amount of transcription of genes. By sequencing these tags, it is possible to measure the expression of thousands of genes simultaneously. Additionally, SAGE can provide quantitative measurements at the gene and/or transcript level, giving researchers much better insight into which genes are being expressed in a given sample. This technique has been used to identify differentially expressed genes in a range of tissues, and can be useful for studying normal and abnormal physiological processes.
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