A primer extension assay is a laboratory method used to study DNA and RNA by extending a short, sequence-specific primer along a template strand using a polymerase enzyme. The primer binds to a known position on the nucleic acid, and the polymerase then adds nucleotides one by one to build a complementary strand. By…
A primer extension assay is a laboratory method used to study DNA and RNA by extending a short, sequence-specific primer along a template strand using a polymerase enzyme. The primer binds to a known position on the nucleic acid, and the polymerase then adds nucleotides one by one to build a complementary strand. By examining how far and how the strand is extended, researchers can map the start sites of transcription, detect specific sequences or modifications, and identify variations such as single-base changes. The technique is a versatile tool in molecular biology for analysing nucleic acid sequence and structure. Within this journal's DNA And RNA Research scope, primer-based and enzymatic methods are central to how nucleic acids are studied, manipulated, and synthesized. Research in this area explores the enzymes and strategies used to copy and build DNA and RNA, the detection of sequence features and mutations, and the broader analysis of nucleic acid structure and function. This page gathers encyclopedic information on the primer extension assay together with peer-reviewed, open-access research relevant to nucleic acid methods and molecular biology within the journal's broader scope.
1 peer-reviewed article, ranked by relevance. Each links to its DOI.
The 1 article above has been cited 2 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
A sample of recent works citing this journal's research on Primer Extension Assay, linking to each citing work.