Peptide Mapping

Peptide mapping is an analytical technique used to accurately identify and characterize proteins. It involves the separation and analysis of peptides derived from a sample protein by using size exclusion chromatography, reversed phase chromatography, or two-dimensional chromatography. The resulting data can be used to gain insight into the protein's structure and can be used as a tool to detect protein modifications or to confirm protein identity. Peptide mapping is a key technique in proteomics and is useful for the development of analytical assays and to identify differences in protein structure and function between conditions or samples. In addition, peptide mapping has become an essential technique in biopharmaceutical quality control and drug discovery.