Subcellular Fractionation

Subcellular fractionation is the process of physically separating different organelles or components of a cell into distinct fractions. This process provides a means to study and analyze the various molecules and components that make up a cell. It can be used to investigate cellular functions, to determine the localization and relative concentrations of cellular components, and to understand cellular processes such as metabolism, protein synthesis, and signal transduction. To achieve this, cells are moved through a series of steps, including homogenization, centrifugation, and filtration. In addition to providing knowledge about the inner workings of cells, subcellular fractionation is also a useful tool for drug discovery, gene therapy, and forensic analysis.