Single Strand Conformational Polymorphism Analysis
Single-strand conformational polymorphism (SSCP) analysis is a technique used to detect genetic mutations. It is based on the principle that strands of DNA that have mutations in their sequence adopt a different conformational structure when subjected to a denaturing environment. The DNA strands are then separated by polyacrylamide gel electrophoresis and analyzed to identify the specific differences. This technique is used to diagnose and detect genetic diseases, as well as to identify polymorphisms related to drug response. It is fast, low-cost and effective in detecting genetic mutations, making it a valuable tool for genetic research.
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