Nested Polymerase Chain Reaction

Nested Polymerase Chain Reaction (nPCR) is a powerful technique used to amplify and detect target DNA sequences in a sample. This method is utilized to accurately detect rare genetic variants and to identify microbial and viral pathogens. nPCR has high sensitivity and is used increasingly in medical diagnostics. It is useful in the detection of genetic diseases and in the identification of pathogens in food or environmental samples. nPCR is also applicable to numerous other areas, such as forensic science, plant breeding, and gene expression studies. In summary, nPCR offers a rapid and highly sensitive method of amplifying and detecting target DNA from a variety of sources.