Gel Filtration

Chromatography Gel filtration chromatography is a type of chromatography used to separate and purify molecules based on their size. It is a chromatographic technique that utilizes a matrix of porous beads, often made of a cross-linked polyacrylamide, to separate molecules. Gel filtration chromatography is commonly used to separate and analyze proteins, peptides, and small-molecular-weight organic compounds (nucleotides, amino acids, carbohydrates, etc.). The separation process is based on the size of the molecules with larger molecules having a higher affinity for the matrix, while smaller molecules pass freely through the beads. Gel filtration chromatography is a fast, efficient, and convenient method for determining the size and purity of proteins, peptides, and small molecules, providing data of critical importance for numerous research and industrial applications.