Blue Native Polyacrylamide Gel Electrophoresis

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) is a technique used in electrophoresis to study the structure, assembly and composition of macromolecules such as proteins. It relies on separating proteins and protein complexes in a gel matrix due to their differences in size and net charge. This technique allows scientists to study the structural properties and topology of large protein complexes, which can lead to a better understanding of the function of these proteins in a cell. BN-PAGE also has applications in drug discovery, as it can be used to identify proteins which may be targeted for therapeutic treatments. Its ability to separate proteins without the need to denature them, means it can provide information about the functional relationship of proteins in their native, active state. As such, BN-PAGE is a widely used tool in biochemistry laboratories.