Affinity Chromatography

Affinity chromatography is a method of separating and purifying proteins and other molecules in a lab. It is based on specific interactions between molecules, such as antigen-antibody interactions, or the interaction between proteins and small molecules or ions. Depending on the type of interaction involved, it can be used to isolate a protein, DNA, RNA, or other molecules of interest. The technique is often used in biochemistry and molecular biology to purify proteins, and is also useful in drug discovery, as it can be used to determine the activity of drug candidates. It has the added advantage of being highly selective, so it can be used to isolate a single type of molecule from a complex mixture.