Affinity Chromatography

Affinity chromatography is a method of separating and purifying proteins and other molecules in a lab. It is based on specific interactions between molecules, such as antigen-antibody interactions, or the interaction between proteins and small molecules or ions. Depending on the type of interaction involved, it can be used to isolate a protein, DNA, RNA, or other molecules of interest. The technique is often used in biochemistry and molecular biology to purify proteins, and is also useful in drug discovery, as it can be used to determine the activity of drug candidates. It has the added advantage of being highly selective, so it can be used to isolate a single type of molecule from a complex mixture.

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Related Articles

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Optimization and ZSPORE Analysis of Affinity Purification Coupled with Tandem Mass Spectrometry in Mammalian Cells

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Liquid Chromatography Tandem Mass Spectrometry Method for Determination of Febuxostat in Human Plasma to Support A Bioequivalence Study

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Rapid and Sensitive High-Performance Liquid Chromatography/Tandem Mass Spectrometry Method for Determination of Danshensu in Rat Plasma

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Glass Chromatography Application: TLC Separation of Benzoic Esters in Pharmaceutical Products

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Phytochemical Analysis and Thin Layer Chromatography Profiling of Crude Extracts from Senna Occidentalis(Leaves)

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Protection Side Chain of Lysine by Host-Affinity Molecules during Solid Phase Peptide Synthesis in Aqueous Phase

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Liquid Chromatography-Mass Spectrometry (LC-MS) Applications in Food Safety–Review

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