Reversed-phase chromatography is a widely used liquid chromatography technique that separates molecules based on their differences in hydrophobicity. In this method, the stationary phase is non-polar, typically a silica support modified with long hydrocarbon chains, while the mobile phase is comparatively polar, oft…
Reversed-phase chromatography is a widely used liquid chromatography technique that separates molecules based on their differences in hydrophobicity. In this method, the stationary phase is non-polar, typically a silica support modified with long hydrocarbon chains, while the mobile phase is comparatively polar, often a mixture of water with an organic solvent such as acetonitrile or methanol. Compounds in a sample distribute between the two phases according to how strongly they interact with the non-polar surface, so more hydrophobic molecules are retained longer and elute later as the mobile phase becomes less polar. This reversed arrangement, compared with normal-phase chromatography, makes the technique especially effective for separating organic compounds, peptides, pharmaceuticals, and natural-product mixtures in aqueous-compatible conditions. Reversed-phase chromatography is a cornerstone of analytical and preparative separations in chemistry, frequently coupled with ultraviolet or mass-spectrometric detection for identification and quantification. Its versatility, reproducibility, and compatibility with gradient elution have made it a standard tool in quality control, method development, and research laboratories. As part of New Developments in Chemistry, this open-access page brings together peer-reviewed research relevant to chromatographic separation methods, analytical chemistry, and the development of new stationary phases and detection approaches.
8 peer-reviewed articles, ranked by relevance. Each links to its DOI.
The 8 articles above have been cited 28 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
A sample of recent works citing this journal's research on Reversed Phase Chromatography, linking to each citing work.