2018
Polysaccharide Transglycosylases: A Survey of Assay Methods
Farkaš VladimírCorresponding author
Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, 84538 Bratislava, Slovakia
Overview
Glycoside hydrolases are enzymes that catalyze the breakdown of glycosidic bonds between carbohydrate molecules or between a carbohydrate and a non-carbohydrate moiety, playing essential roles in numerous biological processes from digestion to cell wall remodeling. Research published in New Developments in Chemistry has examined specialized aspects of these enzymes, particularly focusing on polysaccharide transglycosylases, a subset of glycoside hydrolases that not only cleave glycosidic bonds but also form new ones through transglycosylation reactions. A 2018 article surveyed the methodological approaches used to assay these transglycosylase activities, addressing the technical challenges researchers face when characterizing enzyme specificity and kinetics. This work is significant because accurate measurement of transglycosylase activity is fundamental to understanding how these enzymes modify complex carbohydrates in biological systems and to harnessing their potential in biotechnological applications such as the synthesis of novel oligosaccharides. The ability to reliably detect and quantify both the hydrolytic and synthetic activities of these enzymes remains critical for advancing carbohydrate chemistry and enzyme engineering.
Research published in this journal
1 peer-reviewed article, ranked by relevance. Each links to its DOI.
How this research is being cited
The 1 article above has been cited 2 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
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I. Siziya et al. · 2023 · Food Science and Biotechnology
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2023 · Food Science and Biotechnology
A sample of recent works citing this journal's research on Glycoside Hydrolases, linking to each citing work.