Research Topic · Peer-Reviewed

Anion Exchange Chromatography

Anion exchange chromatography is a form of ion exchange chromatography that separates and purifies molecules according to their net negative charge through reversible electrostatic interaction with a positively charged stationary phase. The chromatographic matrix carries immobilized cationic functional groups, such …

Curated from this journal's research 📚 5 peer-reviewed articles cited Cited 6× across the literature 🔖 ISSN 2377-2549 🗓 Reviewed June 2026

Overview

Anion exchange chromatography is a form of ion exchange chromatography that separates and purifies molecules according to their net negative charge through reversible electrostatic interaction with a positively charged stationary phase. The chromatographic matrix carries immobilized cationic functional groups, such as quaternary ammonium moieties, which bind negatively charged analytes from a loaded sample while uncharged or positively charged species pass through. Bound molecules are subsequently eluted, typically by increasing the ionic strength with a salt gradient or by adjusting the pH so that competing ions displace the analytes or alter their charge, allowing species of differing charge density to be resolved sequentially. Because retention depends on charge, which is governed by pH relative to a molecule's isoelectric point, the technique is widely used to separate and purify proteins, nucleic acids, oligonucleotides, organic acids, and other charged compounds in biochemistry and analytical chemistry. It is valued for its selectivity, capacity, and suitability for both analytical separation and preparative purification, and it is frequently combined with complementary chromatographic modes in multistep purification schemes. Method development centers on selecting the appropriate matrix, buffer, pH, and elution conditions to optimize resolution and recovery. Research involving anion exchange chromatography spans protein and nucleic acid purification, enzyme isolation, analytical separation of charged species, and the development and optimization of chromatographic methods.

Research published in this journal

5 peer-reviewed articles, ranked by relevance. Each links to its DOI.

How this research is being cited

The 5 articles above have been cited 6 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.

A sample of recent works citing this journal's research on Anion Exchange Chromatography, linking to each citing work.

Editorial oversight

Curated from peer-reviewed research published in New Developments in Chemistry (ISSN 2377-2549).

Journal editorial board
Annarita Del Gatto · Italy Bharat Gurale · United States Palani ELUMALAI · United Kingdom

This page summarises published research for orientation; it is not medical or professional advice.