Research Topic · Peer-Reviewed

Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique that separates nucleic acids, and sometimes proteins, by size and charge as they migrate through a porous agarose gel under an applied electric field. Agarose, a polysaccharide derived from seaweed, forms a sieving matrix whose pore size can be tuned by gel conce…

Curated from this journal's research 📚 12 peer-reviewed articles cited Cited 47× across the literature 🔖 ISSN 2377-2549 🗓 Reviewed June 2026

Overview

Agarose gel electrophoresis is a laboratory technique that separates nucleic acids, and sometimes proteins, by size and charge as they migrate through a porous agarose gel under an applied electric field. Agarose, a polysaccharide derived from seaweed, forms a sieving matrix whose pore size can be tuned by gel concentration. Because nucleic acids carry a uniform negative charge, they move toward the positive electrode at rates inversely related to fragment length, allowing samples to be resolved into discrete bands that are visualized with intercalating dyes and ultraviolet or fluorescence imaging and sized against a molecular-weight ladder. The method is a routine step in molecular biology for checking the products of DNA extraction, restriction digestion, the polymerase chain reaction, and cloning, and for confirming amplicon identity prior to sequencing. Research published by the journal applies such molecular and electrophoretic approaches across diverse problems, including proteomic and genomic techniques in cancer diagnostics and personalized medicine, detection of genetically modified crops, characterization of antibiotic-resistance gene mutations in bacterial and tuberculosis isolates, molecular identification of microbial strains, species-level analysis of fish, and the study of gene polymorphisms. These studies illustrate how DNA and protein separation underpins detection, genotyping, and characterization work in the laboratory.

Research published in this journal

12 peer-reviewed articles, ranked by relevance. Each links to its DOI.

How this research is being cited

The 12 articles above have been cited 47 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.

A sample of recent works citing this journal's research on Agarose Gel Electrophoresis, linking to each citing work.

Editorial oversight

Curated from peer-reviewed research published in New Developments in Chemistry (ISSN 2377-2549).

Journal editorial board
Annarita Del Gatto · Italy Bharat Gurale · United States Palani ELUMALAI · United Kingdom

This page summarises published research for orientation; it is not medical or professional advice.