Density Gradient Centrifugation

Density gradient centrifugation is a laboratory technique used to separate components of a mixture based on their density. It works by spinning a sample at very high speeds in a centrifuge tube filled with a gradient of solutions with varying densities. When these solutions are spin in the centrifuge, the components with the highest densities will move toward the bottom of the tube, and those with the lowest densities will move toward the top. This method of separation is useful for separating cell organelles, particles, viruses and proteins from solutions, as well as studying their properties, interactions and dynamics. It is used widely in research in the fields of biochemistry, microbiology, and biophysics.