Journal of Woman's Reproductive Health
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Research Article | Open Access
  • Available online freely | Peer Reviewed
  • Effect of 8 Weeks Exercise on Irisin in Obese Women

    Vahid Sari-Sarraf 1     Saeid Nikoukheslat 1     Zahra Niknam 2      

    1Associate Professor of Exercise Physiology, University of Tabriz

    2PhD candidate of Exercise Physiology, University of Tabriz

    Abstract

    Background

    The prevalence of obesity and type 2 diabetes is escalating at an alarming rate in many developed as well as developing countries. Irisin is a novel muscle and adipose drived chemokine that is, proteolytically processed from the product of the FNDC5 (fibronectin type ш domain containing 5) gene. The purpose of this study is to examine the effect of three kind of training on irisin in sedentary obese women. METHODSː33 obese women (medium age: 37.99 ± 3.7 year, height: 1.55 ± 0.03 meter, BMI: 34.6 ± 5.07 kg/m2) participated in the study, on three groups, including endurance, resistance and concurrent.

    Results

    After 8 weeks exercise we did not find significant differences in fasting glucose, insulin, HOMA-IR and irisin between the groups (P>0.05), but glucose and insulin in resistance groups and irisin in all groups had significant changes (P<0.05).

    Conclusions

    In summery in this study in contrast to hypothesis there were no difference between groups of training. It can be hypothesise that the increase of irisin in obese people is one of the preventing ways against of obesity's side effects. Exercise could improve the signaling pathways and consume the fat accumulations, therefore at the end of exercise duration, irisin decreased.

    Received 27 Aug 2017; Accepted 23 Oct 2017; Published 30 Oct 2017;

    Academic Editor:Qiuqin Tang, Nanjing Medical University

    Checked for plagiarism: Yes

    Review by: Single-blind

    Copyright©  2017 Vahid Sari-Sarraf, et al.

    License
    Creative Commons License    This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Competing interests

    The authors have declared that no competing interests exist.

    Citation:

    Vahid Sari-Sarraf, Saeid Nikoukheslat, Zahra Niknam (2017) Effect of 8 Weeks Exercise on Irisin in Obese Women . Journal Of Woman's Reproductive Health - 2(1):1-9.
    Download as RIS, BibTeX, Text (Include abstract )
    DOI10.14302/issn.2381-862X.jwrh-17-1750

    Introduction

    Discovery of myokines has emphasized the role of muscle as an important source of exercise-induced hormones to communicate information and interact with other tissues, including fat, the liver, and the pancreas, to alter metabolism 1.

    Metabolic diseases such as obesity and diabetes are undoubtedly some of the most challenging health issues of our times 2. The prevalence of obesity and type 2 diabetes is escalating at an alarming rate in many developed as well as developing countries and is basically a consequence of imbalance between energy intake and energy expenditure 3, 4. Obesity on itself could be the source of other disease like type2 diabetes. The prevalence of obesity among the women is more than the men that may be because of the lowest knowledge and lack of exercise and sport facilities in some countries 5, 6. The health benefits of regular exercise are undisputed, thereby reduce the risk of lifestyle-related diseases, such as obesity but the molecular mechanisms are not completely understood 7.

    Irisin is a novel muscle and adipose drived chemokine that is, proteolytically processed from the product of the FNDC5 (fibronectin type ш domain containing 5) gene. Both exercise and the PGC1α (peroxisome proliferator-activated receptor gamma coactivator 1 α) induce irisin expression. Irisin induces the browning of adipocytes and thermogenesis by increasing of UCP1 (uncoupling protein 1) level 8, 9, 10, 11, 12. It is mentioned that irisin could orchestrate many metabolic pathways 13, 14, 15, 16, 17, 18, for example irisin has gained great interest as a potential new target to combat obesity and its associated disorders, such as type 2 diabetes mellitus 10.

    Although circulating irisin was shown to increase in humans in response to exercise 8, but relevance of irisin in human physiology is not fully understood under effect of endurance, strength and in particular concurrent training obscure, and conflicting data in humans have recently emerged, thereby more researches are needed. It has been shown that although circulating irisin is associated with signs of metabolic syndrome and insulin resistance but it is differently regulated by training in normal versus overweight subjects 19, 20, 21, 22, 23, 24, 25, 26. Some mechanism have suggested about the effect of irisin role in glucose uptake and lipid homeostasis 15, 16, 27, 28, 29. In diabetic mice, persistent subcutaneous perfusion of irisin improved the insulin sensitivity, reduced fasting blood glucose, increased GSK3 and Akt phosphorylation, glycogen content and irisin level, and suppressed GS phosphorylation, PEPCK and G6Pase expressions in liver. Irisin improves glucose homeostasis by reducing gluconeogenesis via PI3K/Akt/FOXO1-mediated PEPCK and G6Pase down-regulation and increasing glycogenesis via PI3K/Akt/GSK3-mediated GS activation. Irisin may be taken as a novel therapeutic strategy for insulin resistance and type 2 diabetes 16. But it is necessary to investigate it in different population and under different conditions like exercise, Then in this study it is tried to explore the effect of three kind of isocaloric exercise (Endurance, Resistance and concurrent) on irisin circulation in obese sedentary women.

    Materials and Methods

    This study was carried out during the three month (July to August) in the Northwest of Iran, in the capital city of East-Azerbaijan, Tabriz. At first near the 200 obese women were enrolled by public announcement from different big sectors of the city. Finally according to inclusion criteria and the property of exercise program in many stages by cluster sampling method, all the remained women randomly assigned into endurance, resistance and concurrent exercise training groups on base of obesity. Some of subjects failed during the study and could not complete it. Lastly 33 obese and sedentary women participate in this study (endurance n=10, resistance n=12 and concurrent n=11). Subject characteristics are shown in Table 1. The inclusion criteria required a BMI>25 kg/m2 or a body fat percentage ≥ 30%. Subjects with a history of disease such as major cardiovascular disease, hemodialysis, asthma and other problems were excluded. This study was approved by the ethics committee of IR. TBZMED. REC. 1395. 927. Informed written consent was obtained from each subject. Participants were evaluated before and after the exercise training in all of the measurement (physiologic like irisin, insulin, glucose, HOMA-IR and anthropometric like BMI, Fat% and etc.). BMI (kg/m2)and body composition was determined. Bio-impedance body composition analyzer was used. Also skeletal observed indicated that circulating irisin correlated positively with increased risk of metabolic syndrome and insulin resistance as assessed with these kinds of findings it has been observed higher levels of irisin in overweight pre-diabetes subjects compared with healthy controls 4, 30. Many recent studies in obese patients have shown that irisin decreases with weight reduction. Moreno et al found that irisin was higher in subjects with low activity than in those with average or high activity 37. Huh et al showed that irisin was highest in obese prediabetic patients, followed by obese patients and non-obese individuals, and lowest in athletes 10. The higher irisin in obese persons is due to compensation for metabolic homeostasis, and this suggests that irisin should be decreased by body weight reduction due to discontinuation of compensatory action 14. Due to the difference in metabolism in obese and non-obese individuals, there are disturbances and differences in the signaling processes and intramuscular stimuli of these individuals. Recent investigates suggested that probably irisin has effects on gluconeogenesis and glycogenesis via PI3K/ populations. Irisin secretion in response to adipose and insulin resistance probably enhanced metabolic homeostasis in a compensatory way by altering lipid metabolism via browning of white adiposities 37. Therefore, with regard to one of the important sources of irisin secretion, the adipose tissue, it seems that in this study, the high levels of irisin in obese individuals who did not have a metabolic disorder would have a protective and supportive role in obstructing the complications of obesity, so obese people are less likely to suffer from obesity. In these people, physical activity could decrease the complications of obesity by improving the glucose signaling pathways and other routes, as well as the higher body fat intake, thus reducing Irisin by the end of the period. In general, the present study did not show a significant difference in the effect of different types of training on the Irisin of sedentary and obese women.

    Table 1. subject characteristic in three exercised groups.
    Endurance Resistance concurrent
    Pre Post Pre Post Pre Post
    Age (years)  39.6±3.1 37.1±4.2 37.27±3.8
    Height (m)  1.54±0.03 1.55±0.03 1.56±0.04
    Weight (Kg)  81.62±9.5 80.46±10.8 82.94±10.4 74.09±15.27  85.44±11.01 83.32±11.3
    BMI (Kg/cm2 34.36±4.72  33.87±5.22 34.43±5.58 30.78±6.04 35.01±4.93 34.08±4.5
    %Fat (%)  42.46±4.8  41.89±4.05 44.28±4.2 40.71±5.99 45.31±4.7 44.47±4.05
    VO2max (ml/Kg/min)  21.3±9.46 34.7±7.8 22.7±8.9 36.6±10.13 22.1±8.2 35.44±5.94

    Table 2. Endurance-training program
    Weeks of exercise Intensity of exercise
    1-2 50-60 % VO2max
    3-4 50-60 % VO2max
    5-6 60-70% VO2max
    7-8 60-70% VO2max

    Table 3. Resistance-training program
    Weeks of exercise Intensity of exercise
    1-2 12 reap 2 set 60-65% 1RM
    3-4 10-12 reap 3 set 70% 1RM
    5-6 6-10 reap 3 set 80% 1RM
    7-8 According to recent 4 weeks

    Table 4. Differences among the groups at the beginning of study.
      Mean Square Between Groups Df F P<0.05
    Irisin 0.086 2 2.607 0.092
    Insulin 0.013 2 1.57 0.226
    Glucose 0.001 2 0.298 0.745
    HOMA-IR 0.04 2 1.689 0.203

    This study is not without limitations. We did not any lean control group, however all subjects completed the study in random order then served as their own controls. Also by finding of receptor(s) of irisin in different part of body would open the door to better understand of its role and its mechanisms. Additional researches are needed to elucidate the potential and hidden interaction of irisin and exercise by different intensity and duration in several populations.

    Figure 1. Irisin in different groups
    Figure 1.

    Figure 2. Glucose in different groups
    Figure 2.

    Figure 3. Insulin in different groups
    Figure 3.

    Figure 4. HOMA-IR in different groups
    Figure 4.

    Conclusions

    With regard to one of the important sources of irisin secretion, the adipose tissue, it seems that in this study, the high levels of irisin in obese individuals who did not have a metabolic disorder would have a protective and supportive role in obstructing the complications of obesity, so obese people are less likely to suffer from obesity. In these people, physical activity could decrease the complications of obesity by improving the glucose signaling pathways and other routes, as well as the higher body fat intake, thus reducing Irisin by the end of the period. In general, the present study did not show a significant difference in the effect of different types of training on the Irisin of sedentary and obese women.

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