Journal of Spleen and Liver Research
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Research Article | Open Access
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  • Treatment of Chronic Hepatitis B With Tenofovir At The University Teaching Hospital Campus of Lome (Togo)

    Aklesso Bagny 1       Oumboma Bouglouga 1     Late Mawuli Lawson-Ananissoh 1     Djatougbe AE Akolly 3     Abago Balaka 2     Mohaman awalou Djibril 2     Yao D Atakouma 3     Datouda Redah 1    

    1Departement of gastroenterology, University Teaching hospital Campus of Lomé

    2Departement of Internal Medicine, University Teaching hospital Sylvanus-Olympio of Lomé

    3Departement of Pediatrics, University Teaching hospital Sylvanus-Olympio of Lomé

    Abstract

    Aims:

    To describe the clinical, biological and evolutionary features of mono infected patients treated with tenofovir in Togo.

    Method:

    It is a descriptive, prospective study. Patients were treated with Tenofovir Disoproxil Fumarate (TDF). The inclusion criteria were: active chronic HBV (HBs Ag-positive for more than 6 months, high aminotransferases, the HBV –DNA ≥ 2000 IU / ml for HBeAg negative or ≥ 20 000 IU / ml for HBeAg positive and significant fibrosis) and absence of HCV, HDV, or co-infection HIV.

    Results:

    Among patients with HBV in our department, only 10.68% were treated with TDF. The mean age of patients was 33.01±9.81years. There was male predominance (68%). The circumstances of discovery were mainly during blood donation (65.3%) and a routine checkup (14.7%). Clinical examination was normal in most of cases (86.7%) apart from hepatomegaly (9.3%) and icterus (4%).) The HBeAg was negative in 89.3%; the average DNA was 7.56 ±8.01 log10 IU/ml. Abdominal ultrasonography was performed in all patients and we found hepatomegaly (18.67%), splenomegaly (10.67%), and ascites (5.3%). The assessment of fibrosis and activity had enabled to find a fibrosis higher or equal to 2 in 12 cases (48%) and an activity higher or equal to 2 in 9 cases (36%). The clinical and virologic outcome was marked by an undetectable viral load (HBV-DNA˂10 IU/l) in 89.3% of the patients after 1 year of treatment.

    Conclusion:

    TDF had helped to find out an undetectable viral load in in 89.3% of the patients after one year of treatment.

    Received 17 Jun 2017; Accepted 26 Jul 2017; Published 19 Aug 2017;

    Academic Editor:Khalid Rasheed, University of Alabama at Birmingham

    Checked for plagiarism: Yes

    Review by: Single-blind

    Copyright©  2017 Aklesso Bagny, et al

    License
    Creative Commons License    This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Competing interests

    The authors have declared that no competing interests exist.

    Citation:

    Aklesso Bagny, Oumboma Bouglouga, Late Mawuli Lawson-Ananissoh, Djatougbe AE Akolly, Abago Balaka et al. (2017) Treatment of Chronic Hepatitis B With Tenofovir At The University Teaching Hospital Campus of Lome (Togo). Journal of Spleen And Liver Research - 1(1):16-20.
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    DOI10.14302/issn.2578-2371.jslr-17-1669

    Introduction

    Chronic hepatitis caused by the hepatitis B virus (HBV) affects a high number of people and it was a major cause of mortality and morbidity. It is estimated that approximately 240 million people get chronic infection of hepatitis B virus 1. Chronic viral hepatitis is a serious but underestimated global public health problem 2. Diagnosis and management remain complex and many countries lack the human resources and medical infrastructure to provide treatment. New drugs are now available to treat or to stop the spread of the hepatitis B virus infection, but most people with chronic viral hepatitis are unaware of their infection and do not receive appropriate treatment 3. Up to one third of the chronic viral hepatitis carriers will die of liver cirrhosis or liver cancer if they are not well diagnosed and directed to the appropriate units. Togo is a country located in areas of high endemicity of HBV 4. with high prevalence of chronic HBV-related diseases (43.24%) 5. Despite this fact the country lacks infrastructures and sufficient resources to combate chronic hepatitis B. The new drugs including tenofovir (TDF) are only actually available in our country for 2 years and the cost of treatment is still at the expense of the patient. We, therefore find this study important, to study the clinical, biological and evolutionary features of mono infected patients with HBV treated with TDF in our department.

    Patients And Method

    This is a descriptive prospective study of patients in the department of hepatology and gastroenterology at the teaching hospital campus of Lomé, from february 2012 to july 2015 and treated with TDF for an indefinite period. The only available dose of TDF was administered at a daily dose of 300 mg in the country and is also used for HBV and HIV co-infection. The inclusion criteria were: active chronic HBV (HBsAg positive for more than 6 months, high intermittent or persistent aminotransferases, HBV DNA ≥ 2000 IU / ml for the HBeAg negative or ≥ 20 000 IU / ml for HBeAg positive and necro-inflammation or moderate to severe fibrosis), the absence of HCV, HDV, or HIV co-infection and treatment with tenofovir. We included in this study, patients with a family history of hepatocellular carcinoma and patients with HBV-related cirrhosis. Patients with HDV, HCV or HIV coinfection, inactive chronic carriers of HBV, HBV carriers not treated or treated with another molecule have been excluded. The following parameters were also studied: demographic (age, sex), clinical, biochemical (aminotransferases), serum (HBsAg, total anti-HBc, HBeAg, anti-HBe), virological (DNA of HBV by quantitative PCR). All patients went through abdominal ultrasonography in search of signs of portal hypertension, cirrhosis or hepatocellular carcinoma (HCC). At the level of cirrhosis patients had an upper digestive endoscopy for signs of portal hypertension and determination of alpha fetoprotein in search of hepatocellular carcinoma. The detection of HBsAg was performed according to ELISA sandwich method; as well as the HBeAg, the anti-HBe and the anti-HCV. The DNA of the HBV was measured by the COBAS Ampli Prep technique / HBV COBAS Taq Man Version 2.0 of Roche (Meylan, France) with a positive threshold of 20 IU / ml (linearity from 20 IU / ml to 170.000.000IU / ml). Hepatic fibrosis was assessed by the non-invasive method of the Fibrotest-Actitest. The data were analyzed by SPP 21.0 software.

    Results

    During the period of this study 4410 patients have consulted in our department, including 819 cases of viral hepatitis (18.6%). There were 702 cases of hepatitis B (15.9%) and 117 cases of hepatitis C (2.65%). Among patients with HBV, 75 were put under treatment with TDF (10.68%). The mean age of patients was 33.01±9.81 years old with extremes of 15 and 57 years old. There was male predominance (68%) with a sex ratio of 1.9. The mean age of women was 29 years old against 32.1years old in men (p = 0.058). There was predominance (53.3%) of the age group of 30313233343536373839404142434445; very few of our patients were insured (8%) (Table1).

    Table 1. Demographic data
      Number (n) Percentage (%)
    Averageage 33.1±9.8years old    
    [15-30[ 25 33.3
    [30-45[ 40 53.3
    [45-60[ 10 13.4
    Sex    
    male 51 68
    female 24 32
    Marital status    
    married 23 30.67
    unmarried 52 69.33
    Socio-economic level    
    low 57 76
    average 11 14.7
    high 7 9.3
    Health insurance    
    yes 6 8
    no 69 92
    Home/ Residence    
    Urban 35 46.67
    suburban 13 17.33
    rural 22 36

    Table 2. Circumstances of HBV discovery520065693801000
      Number (n) Percentage (%)
    Blood donation 49 65.3
    Etiological research of cirrhosis 14 18.7
    Health checkup 11 14.7
    Anomaly of the liver tests 08 10.7
    Pre-natal consultation 07 9.3

    Clinically, the circumstances of discovery were essentially during blood donation (65.3%), etiologic assessment of cirrhosis (18.7%) and a routine checkup (14.7%). Clinical examination was normal in most of the cases (86.7%), apart from hepatomegaly (9.3%) and jaundice (4%). Livercheckup had enabled to notice: ALT 72.87±19.4 IU/l, AST 58.88±83.1 IU/l, ALP 190.51±116.5 IU/l, GGT 66.53±68.4 IU/l, albumin 43.54±5.1 g/l, prothrombin 54.76±10.7%, the level of alpha fetoprotein 1.11± 1 ng/ml. The HBeAg was negative in 89.3% of cases; the average DNA was 7.56 ±8.01 log10 IU/ml. Abdominal CT scan was performed in all patients and we found hepatomegaly (18.67%), splenomegaly (10.67%), a dilation of the portal vein(4%), and an ascites (5.3%). The assessment of fibrosis and activity was based on the fibrotest-actitest and had helped to find a fibrosis higher or equal to 2 in 12 cases (16%) and an activity higher or equal to 2 in 9 cases (12%). We got a fibrosis F4 in 4 cases (5.33%). The gastroscopy performed in 9 patients had revealed 3 cases (4%) of esophageal varices of which 3 of grade 2 and a case (1.33%) of grade 3.

    Table 3. Physical examination data
      Number (n) Percentage (%)
    Normal examination 65 86.7
    Hepatomegaly 7 9.3
    Jaundice 3 4
    Ascite 3 4

    Creatinine was stable in all patients, and no cases of kidney failure were reported as tenofovir side effect.

    The clinical and virologic outcome was marked by an undetectable viral load (DNA-HBV˂10 IU/l) in 89.3% of the patients after 1 year of treatment.

    Discussion

    This prospective study is the first to show the results of chronic hepatitis B treatment in Togo. Our methodology is similar to the one of Kissi and al in west Africa6 and that of Nwokediuko and al7 in Asia. Our sample was made of 75 patients with chronic hepatitis B and cirrhosis. This remark is frequent in our practice where most of patients did not know they are hepatitis B virus carriers or they consulted late at the stage of cirrhosis.

    The mean age of patients was 33.01±9.8 years old which clearly shows that the population is young even younger than other studies from West Africa 6, 8. The mean age is higher in Europe than 45 years old 9, 10. This result can be explained by the fact that the contamination of HBV in Africa is most often occurred through mother to child transmission and during childhood. There was male predominance (68%) as in majority of studies in Africa 6, 8, Europe and in Asia 10, 11, 12. The circumstances of HBV discovery varied and were mainly dominated by blood donation and cirrhosis. These results show that, the research of HBV in routine in our context is not systematic, which explains the fact that the diagnosis is made at the stage of complication (cirrhosis) or during a free checkup like blood donation. The systematic research of HBV in routine could help to reduce cases of dangerous complications like cirrhosis or hepatocellular carcinoma. The majority (89.3%) of our patients was HBeAg negative as in most of European studies 13, 14. This high prevalence of the negative HBeAg is the current world wide trend 15 apart from Asia where there is still high prevalences of HBeAg positive 12. The indication of the HBV treatment according to World Health Organization is based on the ALT rate higher than the normal, the DNA of HBV and the severity of the liver disease based on the METAVIR score, APRI score or FIB4, the fibroscan or fribotest-actitest 16. In our study we focused on ALT dosages, the HBV DNA and the fibrotest-actitest. The ALT of our patients was 72.87±19.4 IU/l. This result is comparable to the African data6, but much less than the European and Asian data 12 where we had respectively found 110 IU/l and 155.4IU / l. The average DNA in our study was 7.56 ±8.01 log10 IU/ml. This rate is comparable to that of Kissi and al6 who found 7.4±7.7log10 IU/ml, however it is much higher than the one of Sang Kyung Jun and al 12 who found 4.9±2.3 log10 IU/ml in Kore.

    The assessment of the severity of the disease in our context was based on the clinic in the search of signs of cirrhosis decompensation as jaundice and ascites in 4% respectively. These data were confirmed by abdominal CT scan. The fibrosis was evaluated by a non-invasive test (fibrotest - actitest) as suggested by the majority of the recommendations 18. This test had enabled to find a significant fibrosis in approximately 21.33% of cases and/or a significant activity in 12% of cases. Actually, it has been revealed that most of chronic HBV carriers with HBeAg negative were often discovered late at a stage of liver disease 10. Based on clinical arguments, ultrasonography, endoscopic and non-invasive fibrosis test, we found in our study 5.3% of patients at the stage of cirrhosis before treatment. In Turkey 9 a study had found 3.7% of cirrhotic patients whereas another study in Korean 11 found 51% before the TDF treatment.

    All our patients were naïve to anti-viral treatment. It is said that the anti-viral long-term treatment enables a regression of histological lesions 10. Treatment with TDF is efficient in monotherapy in naïve patients 19 as the results of our study indicated with 89.3% of patient who had an undetectable viral load after one year treatment.

    Conclusion

    The TDF is the first treatment of HBV in our country but the cost of this treatment is still entirely at the expense of the patient.TDF had enabled to find out an undetectable viral load in 58.6% of negative HBeAg patients after a year of treatment. Improving patient care could also include reducing the cost of treatment and implementing treatment in all regions of the country.

    References

    1.Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K. (1990) Aboyans V and al.(2012) Global and regional mortality from 235 causes of death for 20 age groups in. 380(9859), 2095-128.
    2.OMS. (2003) Progrès réalisés sur la voie de la vaccination des enfants contre l’hépatite B. REH,78, 366-370 Document WHO/HSE/PED/HIP/GHP 2012-1.
    3.Hadziyannis S J. (2011) Natural history of chronic hepatitis B in Euro-Mediterranean and African Countries. , Journal of 55(1), 183-191.
    4.Bouglouga O, Bagny A, Djibril M A, Lawson-ananisoh L M.Aspects épidémiologiques, diagnostiques et évolutifs de la cirrhose hépatique dans le service d’hépato-gastroentérologie du. , CHU Campus de Lomé.(2012) J. Rech. Sci. Univ. Lomé 14(2), 1-7.
    5.Djibril M A, Mba K B, Bagnyn A, Kaaga Y L, Gueant J L et al. (2008) Profil etiologique des hépatopathies cytolytiques au CHU Campus de Lomé. , J. Rech. Sci. Univ. Lomé 10(2), 45-48.
    6.Kissi-anzouan-kakou Y H, Doffou S A, Diallo D, Bangoura D A, Adehoumi Y. (2016) . Traitement of chronic hepatitis B with Tenofovir disoproxil fumarate in Ivory coast. Open journal of gastroenterology,6 39-45.
    7.S C Nwokediuko. (2011) Chronic Hepatitis B: Management Challenges. in Resource-Poor Countries.HepatitisMonthly,11 786-793.
    8.Sombié R, Sangaré L, Guingané A, Tiendrébéogo A, Kaboré D et al. (2015) Traitement de l’hépatite B chronique par les analogues de nucléos(t)ides.Journal Africain d’Hépato-Gastroentérologie,9. 114-118.
    9.Örmeci N, Özbaş B, Güner R, Özkan H, Yalçi A et al.and al.(2015) Tenofovir-Best Hope for Treatment of Chronic Hepatitis. , B Infection?The Turkish Journal of Gastroenterology,26 322-327.
    10.Papachrysosa N, Hytirogloub P, Papalavrentiosa L, Sinakosa E, Kouvelisa L. (2015) . Antiviral Therapy Leads to Histological Improvement of HBe Ag-Negative Chronic Hepatitis B Patients.AnnalsofGastroenterology,28 372-376.
    11.Ahn S S, Chon Y E, Kim B K, Kim S U, Kim D Y.. Ahn SH and al.(2014) Tenofovir Disoproxil Fumarate Monotherapy for Nucleos(t)ide-Naive Chronic Hepatitis B Patients in Korea: Data from the Clinical Practice Setting in a Single Center Cohort.ClinicalandMolecularHepatology,20 261-266.
    12.Jung S K, Kim K-Y. (2015) Ha SY,Lee HK,Kim YD,Lee. , B 21-41.
    13.Assi D R, Tenore S B, Pinho J R, Lewis D S, Ferreira P R. (2015) Caracteristics of an outpatient chronic hepatitis B virus infection cohort. 13(2), 189-95.
    14.Gomez R R, Guardiola A A, Gomez Moreno AZ, Garcia Vela A, Gomez Hernando C et al. (2013) Characteristics of patients with chronic hepatitis B virus infection. Analysis of a series of 474 patients. , Gastroenterol 36(4), 243-53.
    15.Wu D L, Xu G H, Lu S M, Ma B L, Miao N Z et al. (2012) Age versus clinical virological characteristics in chronic hepatitis B virus infection :a case series study in China. , Eur J Gastroenterol Hepatol 24(4), 406-13.
    16.Liaw Y F, Sung J J, Chow W C, Farrell G, Lee C Z et al. (2004) Lamivudine for patients with chronic hepatitis B and advanced liver disease NEnglJ. , Med 351, 1521-1531.
    17.Marcellin P, Zoulim F, Hezode C, Causse X, Roche B et al. (2016) . Effectiveness and Safety of Tenofovir Disoproxil Fumarate in Chronic Hepatitis B: A 3-Year, Prospective, Real-World Study in France.DigDisSci 61, 3072-3083.
    18. (2015) World Health Organisation.
    19.Choi M S, Sinn D H, Kim S-A, Lee Y S, Choi W et al. (2012) The Clinical and Laboratory Characteristics of Patients with Chronic Hepatitis B Using Current or Past Antiviral Therapy in Korea: A Multi-Center, Nation-Wide. , Cross-Sectional Epidemiologic Study.Gut 6-241.