Gel Diffusion

Gel diffusion is a technique for separating and purifying molecules and particles with different sizes, shapes and electrical charge in a gel matrix. It works by exploiting the fact that molecules and particles diffuse at different rates in a gel matrix due to their size, shape and electrical charge. As a result, molecules and particles of different sizes, shapes and electrical charge will migrate differently through the gel matrix, allowing them to be separated. Gel diffusion is an important tool in molecular biology, biochemistry and related fields, where it is used to separate proteins, biological macromolecules, cells and more. It has a variety of uses, including purifying proteins and DNA, analyzing protein-protein interaction, identifying and sizing DNA fragments, and more.


From: International Journal of Anesthesia

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