Academic Editor:Marco Innamorati, Milan State University
Checked for plagiarism: Yes
Epigenetics and Nutrition
Epigenetic mechanisms based on DNA methylation, histone modifications and RNA interference have recently showed important association to the development of a wide variety of diseases such as cancer, cardiovascular, metabolic, skin, autoimmune diseases and neurologic disorders. In the context of preventive aspects, the importance of nutrition on epigenetic function has been revealed. Therefore, drastic changes in dietary modifications may contribute to reduced disease risk. For instance, dietary intervention has been showed to affect DNA methylation in Alzheimer’s disease patients. Moreover, maternal high-fat diet can regulate gene expression through promoter histone modifications. Most importantly, RNA interference and particularly micro-RNA mediated regulation of gene expression has been linked to disease development. Remarkably, dietary intake has been demonstrated to significantly affect various miRNAs and their regulation on gene function. In this review, the relationship between epigenetics and disease and development of drugs based on epigenetic targets is presented as well as the influence of dietary intake on epigenetic mechanisms and its effect on disease prevention and therapy will be discussed.
The importance of nutrition in relation to disease prevention and therapeutic interventions has become evident during the past few years. In fact, the daily food intake presents the most important “drugs” used by every one of us 1. Furthermore, the recent progress in genome sequencing and the establishment of nutrigenomics has further revealed the close connection between nutrition and disease 2. In addition, recently nutritional scientists have introduced the term foodomics to describe as the comprehensive high-throughput approach for the exploitation of food science in relation to the improvement of human nutrition 3. In parallel, new findings have closely linked epigenetic mechanisms to disease development, which can be substantially affected by dietary interventions and life-style modifications 4. Therefore, epigenetics has become an important factor also in modern drug discovery and development.
Epigenetic functions can be defined as mechanisms outside the scope of conventional genetic activities and therefore do not generate any modifications of the primary DNA sequence 5. The three main mechanisms of epigenetics are DNA methylation, histone modifications and RNA interference.
Concerning DNA methylations, generally a methyl group (CH3) is covalently added to the 5’-position of cytosine upstream of guanosine affecting gene expression in relation to differentiation, genomic imprinting and DNA repair 6. Typically, the methylated CpG dinucleotides are located in promoter regions and therefore can result in down- or up-regulation of gene expression 7. Aberrant DNA methylation patterns have been linked to disease, particularly cancer 8. Furthermore, inactivation of tumor suppressor genes has been associated with hypermethylation 9. In the context of DNA packaging histones play an important role, which has confirmed their epigenetic association 5. Typically, histones H3 and H4 are modified by a number of mechanisms such as acetylation, methylation, ubiquitination and phosphorylation 10. For example, histone methylation due to increased acetylation is associated with repression or activation of transcription 11. Furthermore, deregulation of histone modifications has been associated with mutations in oncogenes, tumor suppressor genes and DNA repair genes. The third epigenetic mechanism relates to RNA interference and particularly micro RNAs (miRNAs) and their role in the regulation of gene expression 12. The 21,23 nucleotide single-stranded miRNAs interfere with mRNA resulting in the modification of gene expression including both down- and up-regulation 13,14. As at least one-third of human mRNAs are estimated to be regulated by miRNAs they have been frequently linked to cancer and other diseases 15.
In this review, the relationship between epigenetics and disease are described through examples from different disease indications. Furthermore, emphasis is put on recent development of drugs based on epigenetic targets. Attention is also paid to the association of nutrition and epigenetics and how dietary interventions and life-style changes can significantly contribute to reduced health risk and disease prevention.
Due to the close association of epigenetics and disease much attention has been given to evaluate epigenetics in the light of drug targets and the development of novel medicines. In addition to the potential discovery of novel drug mechanisms the reversible nature of epigenetic mechanisms makes them attractive targets for therapy. Epigenetic mechanisms have been linked to a variety of medical indications including cancer, cardiovascular, liver and skin diseases and neurologic disorders (Table 1).Table 1. Epigenetics and Disease.
|RA||miRNA dysregulation||RA pathogenesis||56|
|MS||miRNA dysregulation||disease indicator for MS||57|
|AML||DNA MT inhibition||AML treatment||59|
|Bone||miRNA down-regulation||bone metastasis||26|
|Brain||DNA, H3K27 methylation||ependymoma therapy||100|
|Breast||DNA methylation||cancer association||18|
|Colon||histone modifications||up-regulated acetylation||22|
|CTCL||histone deactylase||CTCL treatment||60 61|
|Glioma||DNA methylation||reduced survival||102|
|Liver||miRNA up-regulation||reduced cell death||104|
|Ovarian||histone modifications||anti-cancer activity||105|
|Prostate||miRNA, tumor suppressor||tumor regression||27 106|
|Stroke||DNA methylation, miRNAs||association with stroke||53 107|
|PH||miR-21 based regulation||control of PH||108|
|CH||HDACs||suppression of CH||109|
|Heart failure||differential expression||heart disease association||110|
|ACHBLF||DNA methylation||disease associated||41|
|NAFLD||differential methylation||inhibition of HSC||111|
|Liver fibrosis||miRNA||HSC phenotype||45|
|NASH||DNA methylation||correlation to NASH||112|
|miR-122||circadian rhythm linkage||113|
|Diabetes||DNA methylation||diabetes association||114|
|histone modification||diabetes protection||115|
|Alzheimer||HDAC inhibitors||reinstated learning||48|
|DNA methylation||reduced pathology||75|
|miR-107, miR-298, miR-328||link to Alzheimer||38 37|
|Parkinson||DNA methylation||Parkinson association||31|
|Psoriasis||DNA methylation||psoriasis pathogenesis||48|
|histone modifications||PASI correlation||48|
|miR.143, miR-223 dysregulation||biomarkers for psoriasis||49|
Altered DNA methylation has been suggested as the epigenetic mechanism associated with cancer 16. For example, hypermethylation of the SPRY2, RASSF1A, RSK4, CHFR and CDH1 genes has been associated with endometrial cancer 17. A genome-wide analysis of DNA methylation in breast cancer and normal tissue suggested that the function and pathways of several genes showed altered methylation status in cancer tissue indicating a role for DNA methylation in breast cancer 18. Similarly, altered DNA methylation patterns have been observed in prostate 19 and rectal 20 cancer patients. Analysis of the DNA methylome of primary breast cancers revealed heterogeneity in transcription activity and differences in metastatic behavior 21. Profiling of the DNA methylome and transcriptome of 44 matched primary breast tumors and regional metastasis revealed divergent changes primarily in CpG island-poor areas.
In the context of histone modifications, it was demonstrated that histone H3 lysine 27 acetylation was up-regulated in colon cancer 22. Moreover, epigenomic-based therapies targeting histone modifications have been developed for the treatment of ovarian cancer 23. In another study, a novel class I histone deacetylase (HDAC) inhibitor MPT0G030 has showed induced cell apoptosis and differentiation in human colorectal cancer cells 24. This in vivo anti-cancer activity suggests a great potential for cancer therapy.
Several studies have revealed the association between miRNAs and cancer. As an example, expression of the p53-specifc miR-34a is capable of inhibition of clonogenic expansion and tumor regression 25. However, when the expression was down-regulated by miR-34a antagomirs, tumor development and metastasis was promoted and prolonged survival in tumor-bearing mice. Down-regulation of miR-143 and miR-145 has been linked to metastasis of the bone 26, whereas miR-200 decrease has been observed in clinical prostate tumors and prostate cancer cell lines 27.
DNA methylation plays an important role in normal brain function and aberrant methylation has recently been linked to neurological disorders and mental illnesses 28. Recently, it was demonstrated that hypermethylation of a 5’ end regulatory region of the gria2 gene correlated with epilepsy seizures in a rat model suggesting that inhibitors of DNA methylation could potentially provide therapy for epilepsy 29. Furthermore, it has been revealed that significant differences in DNA methylation exist between control and Alzheimer patients as well as in animal models for Alzheimer’s disease 30. Similarly, distinctive DNA methylation patterns have been associated with Parkinson’s disease 31. Genome-wide analysis of methylation in brain and blood samples showed substantial differences between patients with Parkinson’s disease and healthy individuals.
Histone modifications have also been linked to Parkinson’s disease. In this context, pathological imbalance between deacetylation and acetylation of histone proteins has been observed in Parkinson patients, which has drawn the attention to HDAC inhibitors for therapeutic applications 32. Likewise, histone modifications have been associated with neurodegeneration in Huntington’s disease 33. Similarly to treatment of Parkinson’s patients, HDAC inhibitors have demonstrated therapeutic efficacy in individuals with Huntington’s disease. Moreover, histone modifications have been suggested to regulate hyperactivity in rats induced by lead exposure 34. Increased histone acetylation was observed after chronic exposure to lead, which provides a better understanding of the etiology of attention-deficit/hyperactivity disorder (ADHD) and potential therapeutic interventions.
Moreover, miRNAs have been linked to Alzheimer’s disease development, where for instance the miR29 cluster targeting beta-secretase BACE1 is down-regulated in Alzheimer brains leading to elevated BACE1 promoting amyloid pathology 35. Likewise, SNPs within miR29 binding sites of the bace1 gene have been associated to sporadic Alzheimer’s disease 36. Furthermore, miR-107, miR-298 and miR-328 target BACE1 and have also been suggested to being linked to Alzheimer’s disease 37,38.
In a recent study, DNA methylation profiles of 59 hepatocellular carcinoma (HCC) patients identified three tumor subgroups, which could be correlated with clinic-pathological parameters 39. Studies on disease progression from chronic hepatitis C to cirrhosis and hepatocellular carcinoma indicated an association with increased DNA promoter methylation 40. The DNA methylation frequency could therefore be used to monitor disease progress and methylation accumulates with progression into cancer. Moreover, aberrant DNA methylation of the G protein-coupled bile acid receptor 1 (Gpbar1) promoter has been linked to acute-on-chronic hepatitis B liver failure (ACHBLF) 41. In this context, the frequency of the Gpbar1 promoter methylation was significantly higher in ACHBLF patients compared to individuals with chronic hepatitis B.
Related to histone modifications there are a number of suggestions linking them to liver disease 42. For example, histone modifications have been associated with alcoholic liver disease 43. Additionally, inactivation of the tumor suppressor gene RIZ1 in hepatocellular carcinoma involved histone H3 lysine 9 (H3K9) modifications in combination with DNA methylation 44.
A number of miRNAs have been identified in regulating proliferation, apoptosis, TGFβ1 signaling, and collagen expression of the hepatitis stellate cell (HSC) phenotype and progression of fibrosis 45. Moreover, differential expression of 100 miRNAs has been demonstrated in non-alcoholic steatohepatitis (NASH) 46. Several miRNAs control lipid and glucose metabolism and such as miR-122 has showed close linkage to the circadian rhythm 47. Also, miR-122 expression is down-regulated in NASH patients and functionally implicated in in mice with non-alcoholic fatty liver disease (NAFLD).
Epigenetic mechanisms have also been linked to a number of other diseases. For instance, the DNA methylation pattern for multiple genes involved in psoriasis pathogenesis is abnormal and hypoacetylation of histone H4 was observed in peripheral blood mononuclear cells from psoriasis patients 48. Furthermore, miRNAs have been linked to psoriasis, particularly miR-223 and miR-143 showing differential expression in psoriasis patients 49. Epigenetic differences have also been observed in obese and diabetic individuals 50. In this case, epigenetic mechanisms have been associated with maternal diabetes mellitus 51. Related to type 1 diabetes histamine deacetylase 3 (HDAC3) inhibitors protect β-cells from cytokine-induced apoptosis 52. In the case of type 2 diabetes, HDAC3 regulates genes involved in insulin resistance and pancreatic β-cell failure and HDAC3 inhibitors could therefore provide an attractive therapeutic strategy. Furthermore, in stroke patients the total DNA methylation of the tumor necrosis factor-α (TNF-α) promoter was lower 53. Epigenetic function by miRNAs has also been associated with autoimmune disease and rheumatoid arthritis 54. In this context, miR-124 was demonstrated to regulate the proliferation and secretion of monocyte chemoattractant protein-1 (MCP-1) and dysregulation might cause inflammatory pathogenesis 55. Additionally, Let-7a and miR-132 have been linked to rheumatoid arthritis pathogenesis 56 and miR-21 an indicator of progress of multiple sclerosis 57.
Progress in understanding epigenetic mechanisms and their link to disease has accelerated the development of novel epigenetic drugs. Several epigenome-targeted drugs have already been approved by the US Food and Drug Administration 58. In this context, the DNA methyltransferase inhibitors azacitidine and decitabine have been approved for the treatment of acute myeloid leukemia (AML) 59. Moreover, two HDAC inhibitors, vorinostat 60 and romidepsin 61, are used for cutaneous T-cell lymphoma (CTCL) treatment. Additionally, several epigenetic drugs have been demonstrated to enhance memory function in rodents. Therapeutic efficacy has been observed for Alzheimer’s disease, Schizophrenia and depression in animal models 62. Evaluation of HDAC inhibitors in a murine model of the cyclin-dependent kinas 5 activator p25 protein reinstated learning behavior and synaptic plasticity even after severe neuronal loss 63.
Related to liver diseases epigenetics has provided some considerable impact on drug development 64. For instance, the DNMT1 inhibitor 5-azadeoxycytidine (5-AzadC) and the EZH2 inhibitor 3-deazaneplanocin A (dZNep) are potent inhibitors of HSC activation 65. Furthermore, in 76% of NAFLD patients differentially methylated CpG sites became hypomethylated in advanced disease whereas 24% underwent hypermethylation 64.
In the context of drug development, genetic and epigenetic variations between individuals have triggered the need of designing personalized medicines. Diagnostic biomarkers play an important role in this context, particularly in the prediction of clinical drug responses before treatment 66. This type of pre-screening will allow for disease-specific therapy without the need of subjecting patients to drugs unlikely to provide any clinical benefit. In the case of oncology, isolation and analysis of genetic, biochemical and immunohistochemical markers from tumors and biofluids aid in defining selective treatment 67. Investigation of 19 candidate genes demonstrated that hypermethylation of the AKR1B1 and TM6SF1 promoters can serve as biomarkers for the early detection of breast cancer 68. Likewise, methylation profile studies of 59 patients with hepatocellular carcinoma identified three tumor subgroups, which correlated with clinic-pathological parameters 69.
Furthermore, histone-modifying genes have also been evaluated as diagnostic markers. For instance, studies on HCC and adjacent non-cancerous patient tissue revealed by RT-qPCR and tissue microarray-based immunohistochemistry (TMA-based IHC) analysis showed up-regulation of the histone phosphorylation gene ARK2 and methylation genes G9a, SUV39H2 and EZH2 70. Clearly, overexpression of EZH2 and SUV39H2 was associated with HCC prognosis and could serve as novel prognostic biomarkers. Another approach has been to apply imaging mass spectrometry to identify modified forms of histone H4 as new biomarkers of microvascular invasion in HCC 71. The analysis showed that 28 of 30 differential protein peaks were overexpressed in HCC patients, of which two peaks were identified as N-terminal acetylated histone H4 dimethylated at lysine 20 and acetylated at lysine 16, respectively.
There are plenty of indications that dietary intake has a strong influence on epigenetics 72 (Table 2). When rodents are subjected to a diet depleted of methyl donors it promotes DNA hypomethylation and the development of steatosis 73. In contrast, a high-calorie diet supplemented with methyl donors prevented NAFLD suggesting that epigenetic modifications which affected hepatic fat metabolism were related to DNA methylation changes.
Another example is the potential link of nutrition to histone modifications and alcohol-induced liver disease (ALD) 74. Particularly, critical metabolites such as acetate, S-adenosylmethionine (SAM), nicotinamide adenine dinucleotide, and zinc are relevant to alcohol metabolism and ALD. Furthermore, high SAM levels are boosted by a folic acid rich diet, which has been showed to reduce pathology in a mouse model for amyloid pathology 75. Similarly, vitamin deficiency was observed in mice suffering from memory impairment 76. For instance, vitamins and minerals play an important role in miRNA modulation and the potential of viable exogenous miRNAs entering human blood circulation from food sources has added a new dimension to the impact of nutrition on miRNAs and its effect on health 77. Related to breast cancer, selenium has proven promising as an anti-breast cancer trace element affecting DNA methylation and histone modifications 78. Treatment of MCF-7 human breast carcinoma cells with methylselenic acid or selenite resulted in a dose-dependent inhibition of cell proliferation.Table 2. Epigenetics and Nutrition.
|Nutritional Source||Epigenetic function||Disease/Effect||Reference|
|Polyphenols||miR-103/107, 122||Fatty liver disease||116|
|Caffeic acid, hesperidin||miR-30c, 291, 296, 374, 476b||miRNA modulation ApoE mice||117|
|Proanthocaynidines||miR-30b, 197, 523-3p, 1224-3p||Human HepG2 cells||118|
|HFD (60% kcal fat)||miR-21, 142, 146||Obesity/miRNA modulations||119|
|HFD (58% kcal fat)||20 miRNAs||Diabetes/miRNA modulations||120|
|Methyl-depleted diet||DNA methylation||Hypomethylation, steatosis||73|
|Selenium||DNA methylation||Anti-breast cancer activity||78|
|Selenium||histone modifications||Anti-breast cancer activity||78|
|Folic acid||histone modifications||Reduced amyloid pathology||75|
|Vitamins||DNA methylation||Memory impairment||76|
|Vitamins, minerals||miRNAs||miRNA modulation||77|
|Milk||miRNAs packaged in exosomes||Regulation of IgA immune network||86|
|Milk/human PNBC||miR-29b||Transcription factor expression||86|
|Milk/C57BL/6J||miR-29b||Decrease in miR-29b concentration||86|
|Tobacco smoke||DNA methylation||Biomarkers||87|
|Solanaceous plants||2239 miRNAs identified||Link to transcription, metabolism||88|
|2-AAF||miR-34, 200a/200b||DNA damage, oxidative stress||122|
|Fish oil||miR-16, 19b, 21, 26b||miRNA modulations||123|
|Pectin||miR-27, 93, 203||miRNA modulations||123|
Recently, the understanding of the relationship between epigenetics, ageing and nutrition has been enlightened by a number of new findings. Emerging evidence suggests that long term changes in DNA methylation during early life, especially related to nutrition, can cause altered susceptibility to a variety of diseases associated with ageing 79. One aspect of early life nutrition is breast feeding, which is well-known for its favorable effects in preventing acute and chronic diseases 80 and association with better neuronal-behavioral development 81. Epigenetic effects of human breast milk might occur and several components of breast milk have been linked to epigenetic changes. In this case, lactoferrin has been associated with reduced NF-κB expression contributing to the prevention of disorders of the immune system 82. Likewise, prostaglandin J enhances peroxisome proliferator-activated receptor gamma (PPARɣ) expression and has been associated with obesity prevention 83. However, additional studies have to be conducted to further determine the link between human breast milk and epigenetic functions. Furthermore, intra-uterine development has been showed to influence health outcome in adult life and epigenetic regulation has been implied 84. In this context, the impact of prenatal drug and substance exposure on childhood development has received plenty of attention, but even life-long health outcome due to epigenetic memory has generated interest.
In the context of epigenetics and breast milk, miRNAs packaged in exosomes in porcine milk have been suggested to influence development in piglets 85. Isolation of exosomes from porcine milk identified 176 known miRNAs and 315 novel mature miRNAs. Genome pathway analysis indicated that some miRNAs targeted genes enriched in transcription, immunity and metabolism and may be involved in the regulation of the IgA immune network. Moreover, a number of miRNAs encapsulated in exosomes have been isolated from bovine milk 86. As the majority of bovine miRNA sequences are complementary to human transcript genes, there is a possibility that bovine milk miRNAs might regulate human gene expression. Analysis of peripheral blood mononuclear cells in milk drinkers demonstrated that the expression of runt-related transcription factor 2 (RUNX2), a known target for miR-29b, increased 31%. Addition of milk exosomes to mimic postprandial concentrations of miR-29b and miR-200c decreased reporter gene expression by 44% and 17%, respectively in human embryonic kidney 293 cells. Likewise, C57BL/6J mice fed on a milk miRNA-depleted diet for four weeks showed a 61% decrease in miR-29b concentration. In contrast, broccoli sprout feeding elicited no increase in specific miRNA activity. Although additional studies are required, it can be postulated that miRNAs in milk can act as bioactive food compounds in the regulation of human genes.
Exposure to tobacco smoke was analyzed for the identification of short- and long-term DNA methylation in two cohort studies 87. Based on genome-wide DNA methylation profiles it was possible to establish biomarkers for short-term and lifelong tobacco smoke exposure.
Genome-wide analysis of solanaceous plants (potato, tomato, tobacco, eggplant, pepper and petunia) identified 2239 miRNAs 88. Bioinformatics suggested that miRNAs were linked to 620 targets, which were classified as transcription factors, metabolic enzymes, and RNA and protein processing enzymes involved in plant growth and development. Furthermore, the recent discovery of oral intake of plant miRNAs through food consumption and its direct influence of gene expression in mice 89 has received much attention. The question is: do we eat gene regulators 90? In support, exogenous plant miRNAs was detected in sera and tissues in animals fed on plants 91. For instance, miRNAs abundant in rice such as miR-168a has been demonstrated to bind to the human and mouse low-density lipoprotein receptor adapter protein 1 (LDLRAP1) mRNA. As decreased LDL removal from the plasma corresponds to inhibition of LDLRAP1 expression, exogenous food-derived plant miRNAs might potentially regulate gene expression in mammals. However, recently another study suggested that although substantial amounts of miRNAs in the diet are commonly consumed orally by humans, mice and honey bees, healthy athletes did not show detectable miR-156a, miR-159a and miR-169a levels in the plasma 92. Likewise, when mice received a diet with animal fat rich in endogenous miR-21 only negligible miR-21 was observed in the plasma and organ tissue. Furthermore, plant miRNAs subjected to oral pollen uptake showed hardly any presence in honeybees. Horizontal delivery of miRNA through food intake therefore seems to be rather infrequent.
Interestingly, in a recent study the expression levels of a panel of seven human miRNAs were analyzed in plasma and stool samples of individuals with different dietary habits (vegans, vegetarians and omnivores) 93. Clearly, miR-92 was differentially expressed in both plasma and stool samples of individuals with different diet intake indicating miRNA modulation by nutrition.
The impact of nutrition on health in general and the effect of changes in nutrition and life-style on disease development have already been described above. Furthermore, it is important to address the psychosocial factors related to nutrition and their impact on health and disease prevention. In case of weight loss success, women with morbid obesity were subjected to cognitive-behavioral methods including education of exercise and healthy eating practices 94. The study results suggested that the treatment-induced psychosocial changes might be advantageous for more successful behavioral weight-loss management. Moreover, the global concern of the dramatic increase in childhood obesity has been demonstrated to be affected by psychosocial factors such as psychological, environmental and sociocultural factors, which might include above-average food intake of low nutritional quality 95. In another study in adults with severe obesity the temporal aspects of psychosocial predictors were assessed on increased fruit and vegetable intake 96. Significant improvement in self-regulation, mood, self-efficacy, fruit and vegetable intake, and physical activity were observed after 6 months in individuals receiving behavioral support in education for physical activity and nutrition intake. Furthermore, changes to increase fruit and vegetable intake in relation to psychosocial factors were investigated in a Malaysian study, which suggested that intervention strategies should emphasize on increasing perceived benefits and establishing self-efficacy 97. This can be achieved through better knowledge and skills of consuming a diet rich in fruits and vegetables as the mean of promoting healthy living.
The behavior to reduce the risk of obesity risk in relation to psychosocial factors was recently investigated in US- and foreign-born Chinese Americans 98. The main reasons for the prevention of adopting obesity risk-reducing behavior related to the convenience of consuming fast food meals, cost, lack of time to prepare home-cooked meals, and the physical environment of unhealthy food. The “Western-identified” individuals showed a significant attitude to promote obesity risk-reduction behavior, whereas “Asian-identified” persons expressed a perceived behavioral control, self-efficacy and the observed benefits were important. Moreover, the effect of nutrition- and health-related psychosocial factors and the association to diet, exercise and weight status was studied in 4356 adults in the US 99. The results indicated small ethnic differences, but the socioeconomic status differences were significant.
Epigenetic mechanisms have been frequently linked to a wide spectrum of disease indications and therefore provide novel and attractive means for therapeutic interventions. In this context, a number of drugs mainly based on histone deacetylase inhibitors have already been approved. The reversible nature of epigenetic functions is an additional attraction for drug development. Furthermore, epigenetics have proven fruitful for the generation of diagnostic biomarkers. In this context, DNA methylation and histone modifications have served as biomarker targets, but especially a number of miRNAs can provide valuable prognostic information.
The influence of nutrition on epigenetic mechanisms has received much attention lately. Food intake has been linked to changes in DNA methylation and histone modifications. Moreover, the presence of miRNAs in the diet has been suggested to affect gene regulation. In this context, miRNAs are encapsulated in exosomes in milk, which have been demonstrated to increase transcription factor expression in peripheral mononuclear blood cells. Recently, some evidence suggests that food-derived plant miRNAs might regulate gene expression in mammals. However, contradictory results indicated that oral uptake of plant miRNAs was hardly detectable in the plasma of mice and men.
In conclusion, epigenetics presents a novel approach for modern drug development and even more importantly the influence by dietary interventions on epigenetic mechanisms further strengthens the importance of nutrition on health and disease risk, and particularly holds a key role in preventive medicine and improved health for the humankind at a reduced cost in comparison to long-term drug-based treatment of chronic disease.