Polyethylene Glycol Precipitation

Polyethylene glycol (PEG) precipitation is a process used to purify, concentrate and remove unwanted components in biological samples. It is a common technique in molecular biology, biochemistry and proteomics. PEG precipitation is based on the principle that when a protein is mixed with a high concentration of PEG, it precipitates out of the solution and can be collected by centrifugation. This technique reduces the presence of salts and impurities, increases protein yield, reduces sample complexity and can be used to separate enzymes from other components. PEG precipitation is a simple, fast and cost-effective method for protein and nucleic acid purification.