Denaturing Gradient Gel Electrophoresis

(DGGE) Denaturing Gradient Gel Electrophoresis (DGGE) is a powerful tool used in molecular biology for the detection and visualisation of variations between genetic sequences. It works by separating single-stranded DNA molecules of different sequences under the influence of an electric field, while a denaturing gradient of an organic solvent (e.g. formamide) is present. It is used to analyse microbial community structure and diversity, to identify mutations in genes, and to study DNA binding proteins. DGGE is a powerful method of DNA fingerprinting, providing high resolution images which help in the detection of subtle variations. It is also used to assess genetic constraints in the development of disease.