Reverse Transcription

Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a powerful tool used in molecular biology to detect and quantify the levels of specific DNA and RNA molecules. It relies on the process of reverse transcription of a RNA template, followed by the amplification of a complementary DNA strand (cDNA) through polymerase chain reaction (PCR). RT-PCR can be used to detect the presence of a specific gene, measure the expression of a gene, detect changes in gene expression, and quantify the amount of target RNA in a sample. It is commonly used in diagnostics for medical conditions, for research, and in numerous industries.