Ligation Independent Cloning

Ligation Independent Cloning (LIC) is a simplified molecular cloning method used in molecular biology and biotechnology. It streamlines the molecular cloning process by using a single step to introduce a new gene into a vector, allowing for the fast and efficient manipulation of DNA sequences. LIC is used in a variety of applications, such as protein expression, gene therapy, and vaccine production. By allowing for the fast and efficient manipulation of genetic sequences, this technology helps speed up the process of developing life-saving treatments and diagnostic tests.