Name: Sergey Sinenko

Country: Russian Federation

Affiliation:

INSTITUTE OF CYTOLOGY of the Russian Academy of Sciences

Email: Send an Email


Address:

Laboratory of Molecular Biology of Stem Cells INSTITUTE OF CYTOLOGY of the Russian Academy of Sciences Tikhoretsky ave. 4,194064, St-Petersburg, Russia Тel: +7 (812) 297 4519 
 Fax: +7 (812) 297 3541 Tel: +7 (911) 214-4807

Research Interests:

Institute of cytology of the Russian Academy of Science, St-Petersburg, Russia 5/2014 - present Staff Research Scientist, Laboratory of Molecular Biology of Stem Cells Human artificial chromosomes in stem cell research and technology Molecular mechanisms of cell reprogramming to pluripotent stem state Duke University School of Medicine, Durham, NC 7/2012-4/2014 Staff Research Scientist, Department of Pharmacology and Cancer Biology Molecular mechanisms of apoptosis in Drosophila – Design and perform a modifier genetic screen and identified a novel deubiquitinase which acts as proapoptotic factor in Drosophila apoptosis. – Analyzed and determined the function of a novel deubiquitinase enzyme DUBAI in stabilizing DIAP1 in vivo, in Drosophila. – Develop and carry out modifier genetic screen, and identify genomic intervals and single alleles that involved in process of metabolic (NADPH-dependent) regulation of Dronc. – Collaborate with Dr. Yan’s lab and characterize function of fly dIDH-R195H mutant in apoptosis and fly blood development. University of California Los Angeles, CA 7/2004 – 2/2011 Postdoctoral Research Associate in Department of Molecular, Cell, and Developmental Biology Molecular mechanisms of hematopoietic development in Drosophila model – Identified the function of Wg/Wnt signaling pathway in the maintenance of blood progenitor cells in Drosophila. – Designed the modifier genetic screen and identified several genes interacting with AML1-ETO oncogenic pathway. – Identified that ROS-mediated activation of EGFR ligand in the hematopoietic niche regulates development of cellular immune response in Drosophila. – Investigated Pvr and STAT signaling in Drosophila hematopoiesis. Harvard Medical School, Dana-Farber Cancer Institute, Boston, MA 9/2000 –6/2004 Postdoctoral Research Associate in Department of Pediatric Oncology Molecular mechanisms in Drosophila blood cell development – Identified an evolutionary conserved gene, yantar (ytr), that function as a tumor suppressor during blood development in Drosophila. – Provided genetic and molecular evidence that ytr function is required for proper development of blood cells in Drosophila. – Determined that the ytr gene genetically interacts with the tetraspanin gene, Tsp68C. – Designed hemocyte specific Gal4 lines, suitable for efficient expression of gene of interest in Drosophila hematopoietic tissue. Institute of Genetics, BRC, Hungarian Academy of Science, Szeged, Hungary. 10/1999-8/2000 Research Fellow at International Training Course: Identification of different hemocyte populations and their precursors in Drosophila – Developed six monoclonal antibodies directed against distinct molecular markers on Drosophila hemocytes. – Characterized hemocyte specific proteins with the novel monoclonal antibodies. These antibodies are currently being used to type the various hemocyte subsets in Drosophila blood and to molecularly clone identified molecular markers. Institute of Molecular Biology and Biochemistry, Almaty, Republic of Kazakhstan, 1992-10/1999: Junior Research Associate / Graduate Student Thesis: Epitope analysis and development of ELISA tests by using newly developed monoclonal antibodies to human alpha-fetoprotein and choriogonadotropin. – Developed a panel of monoclonal antibodies (MAbs) to human alpha-fetoprotein (hAFP), and performed epitope mapping of hAFP molecule. – Designed monoclonal antibody-based ELISA diagnostic tests to human chorionic gonadotropin (hCG). – Developed ELISA tests to staphylococcal enterotoxin A (SEA). http://www.researchgate.net/profile/Sergey_Sinenko/

Biography:

1. S.A. Sinenko. Proapoptotic function of deubiquitinase DUSP31 in Drosophila. Oncotarget. 2017 Jul 31; 8(41): 70452-70462. 2. Z.J. Reitman, S.A. Sinenko, E.P. Spana, H, Yan. Genetic dissection of leukemia-associated IDH1 and IDH2 mutants and D-2-hydroxyglutarate in Drosophila. Blood. 2015 Jan 8;125(2):336-345. 3. C.-S. Yang, S.A. Sinenko, M.J. Thomenius, A.C. Robeson, C.D. Freel, S.R. Horn and S. Kornbluth. The deubiquitinating enzyme DUBAI stabilizes DIAP1 to suppress Drosophila apoptosis. Cell Death and Differentiation. 2014 Apr; 21(4): 604-611. 4. S.A. Sinenko, J. Shim and U. Banerjee. Oxidative stress in the haematopoietic niche regulates the cellular immune response in Drosophila. EMBO reports. 2012 Jan 1; 13(1): 83-89. 5. B.C. Mondal, T. Mukherjee, L. Mandal, C.J. Evans, S.A. Sinenko, J.A. Martinez-Agosto and U. Banerjee. Interaction between Differentiating Cell- and Niche-Derived Signals in Hematopoietic Progenitor Maintenance. Cell. 2011 Dec 23; 147(7), 1589-1600. 6. S.A. Sinenko, K.W. Hung, T. Moroz, Q-M. Tran, M.D. Cheney, N.A. Speck and U. Banerjee. Genetic manipulation of AML1-ETO-induced expansion of hematopoietic precursors in a Drosophila model. Blood. 2010 Nov 25; 116(22): 4612-4620. (ranked by Faculty of 1000) 7. S.A. Sinenko, L. Mandal, J. Martinez and U. Banerjee. Dual role of Wingless signaling in stem-like hematopoietic precursor maintenance in Drosophila. Developmental Cell. 2009, 16, 756-763. 8. C.J. Evans, S.A. Sinenko, L. Mandal, J.A. Martinez-Agosto, V. Hartenstein and U. Banerjee. Genetic dissection of hematopoiesis using Drosophila as model system. Cardiovascular Development, Volume 18: Advances in Developmental Biology, Elsevier B.V., 2007, 259-299. 9. Rus F, Kurucz E, R. Markus, S.A. Sinenko, B. Laurinyecz, C. Pataki, J. Gausz, Z. Hegedus, A. Udvardy, D. Hultmark and I. Ando. Expression pattern of Filamin-240 in Drosophila blood cells. Gene Expression Patterns. 2006 Oct; 6(8): 928-934. 10. S.A. Sinenko and B. Mathey-Prevot. Increased expression of Drosophila tetraspanin, Tsp68C, suppresses the abnormal proliferation of ytr-deficient and Ras/Raf-activated hemocytes. Oncogene. 2004, Dec 2, 23(56), 9120-9128. 11. S.A. Sinenko, E. K. Kim, R. Wynn, P. Manfruelli, I. Ando, K. A. Wharton, N. Perrimon and B. Mathey-Prevot. Yantar, a conserved arginine-rich protein, involved in Drosophila hemocyte development. Developmental Biology. 2004 Sep 1; 273(1): 48-62. 12. S.A. Sinenko and N.N. Belyaev. The epitope analysis of human alpha-fetoprotein with novel monoclonal antibodies. Reports of National Academy of Sciences, Republic of Kazakhstan, 2002 (3): 57-62 (English). 13. N.N. Belyaev, S.A. Sinenko and R.T. Tleulieva. The influence of the space flight factors on the protein synthetic and metabolic activity of animal cell culture. Space Investigations in Kazakhstan, Rondo, Almaty, 2002: 408-411. 14. S.A. Sinenko, N.N. Belyaev, V.D. Kuzovlev, A.A. Khakimzhanov. The immunochemical distinctions of human alpha-fetoprotein preparations as revealed by novel monoclonal antibodies. Reports of National Academy of Sciences, Republic of Kazakhstan, 1999 (1): 72-80 (English). 15. S.A. Sinenko. Monoclonal ELISA test-systems to human alpha-fetoprotein. Methods of Molecular Biology, Biochemistry and Immunochemistry. Almaty, 1999: 126-32 (Russian). 16. S.A. Sinenko, N. N. Belyaev. Generation of antibodies against human chorionic gonadotropin and development of an ELISA test-system. Biotechnology - Theory and Practice, 1997: (2) 20-5 (Russian). 17. R.T. Tleulieva, S.A. Sinenko, N.N. Belyaev. Monoclonal antibody to staphylococcal enterotoxin A and the immunoenzyme test-system on their basis. J. Microbiologia 1997: (3) 56-8 (Russian).