Reverse Transcription

Polymerase Chain Reaction Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that amplifies a specific target DNA or RNA sequence. This amplification process is achieved by cycling the reverse transcription and polymerase chain reaction (PCR) steps, in which a template strand is transcribed into complementary DNA, then PCR is used to amplify the target sequence. RT-PCR is a crucial tool in molecular biology and is widely used in the diagnosis and monitoring of many diseases, such as cancer, genetically inherited disorders and viral infections. RT-PCR is a powerful tool that allows scientists to accurately and quickly quantify small amounts of genetic sequences.