Restriction Fragment Mapping

Restriction Fragment Mapping is a powerful laboratory technique used to map the sequence of individual genes or entire genomes. By using restriction enzymes to cleave DNA at specific restriction sites, DNA fragments are generated, which can be separated by size and analyzed using gel electrophoresis. This generates a unique restriction fragment map that can be used to compare the sequence of samples and figure out the exact order of DNA bases. This technique has many practical applications, such as identifying mutations, diagnosing diseases, and reconstructing evolutionary relationships.