Forms of Dna
Sequencing DNA sequencing is a process of determining the exact order of nucleotides within a DNA molecule. It is used to acquire detailed genetic information, such as mutations that may cause diseases, to understand the structure and dynamics of the genome, and to develop new treatments and diagnostics for diseases. The most commonly used forms of DNA sequencing are Sanger, 454, and Illumina. Sanger sequencing is an older method that uses chemical lab techniques to sequence the four bases A, T, G and C of a given region of DNA. 454 sequencing is a newer method that uses a combination of PCR and sequencing-by-synthesis reactions to sequence a much longer region of DNA than Sanger sequencing. Illumina sequencing is a third form of DNA sequencing that uses a sequencing-by-synthesis approach to create very large sequences of DNA. Its high throughput and accuracy make it the preferred form of DNA sequencing used in most research and clinical settings.
← Journal of DNA And RNA Research