Overview
Directional cloning is a process used to insert a DNA fragment into a vector for the purpose of replicating and cloning genes for research, diagnosis or disease treatment. The DNA fragment is inserted into the vector in a specific direction, allowing researchers to control which strand of DNA is expressed. It is very useful in the development of recombinant proteins, as it gives researchers the ability to control the sequence of the protein. It can also be used to study gene expression, as only the properly oriented strand will be expressed in the final product. Furthermore, directional cloning is used in the production of vaccines, therapeutic proteins and gene therapies, making it a key tool in the advancement of biotechnology.
Research published in this journal
2 peer-reviewed articles, ranked by relevance. Each links to its DOI.
How this research is being cited
The 2 articles above have been cited 7 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
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2023 · ChemBioChem
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2022 · ChemBioChem
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2019 · Nature Ecology & Evolution
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2019 · Nature Ecology & Evolution
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2017 · International Journal of Environment Agriculture and Biotechnology
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M. Chliyeh et al. · 2017 ·
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2016 · Journal of DNA And RNA Research
A sample of recent works citing this journal's research on Directional Cloning, linking to each citing work.