Journal of Clinical Research In HIV AIDS And Prevention

Journal of Clinical Research In HIV AIDS And Prevention

Journal of Clinical Research in HIV AIDS and Prevention

Current Issue Volume No: 2 Issue No: 2

Case Report Open Access Available online freely Peer Reviewed Citation

Report of a False Positive Rapid HIV Test Due to Hepatitis A in a U.S. Army Soldier

1782nd BSB, 4th Brigade, 82nd Airborne Division, Fort Bragg, NC, USA

2Department of Clinical Investigation, Womack Army Medical Center, Fort Bragg, NC, USA

3Departments of Medicine and Pediatrics, Womack Army Medical Center, Fort Bragg, NC, USA


A 25 year old, single, active duty soldier presented to a clinic in Afghanistan complaining of malaise, fatigue, acholic stools, and mild jaundice over a 5- to 7-day period. He had significantly elevated liver transaminase levels approaching 5000 U/L and a positive rapid human immunodeficiency (HIV) 1 antibody test. Ultimately, the patient was found to have a false positive rapid HIV-1 antibody test due to acute hepatitis A virus infection. This case report describes his evaluation and outcome, in addition to exploring possible causes of false positive HIV screening.

Author Contributions
Received 31 Jul 2014; Accepted 16 Aug 2014; Published 6 Oct 2014;

Academic Editor: Christophe Marchand, Staff Scientist, Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, NIH

Checked for plagiarism: Yes

Review by: Single-blind

Copyright ©  2014 Contrina A Huffman, et al.

Creative Commons License     This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Competing interests

The authors have declared that no competing interests exist.


Contrina A Huffman, Rachel N. Barts, Cristóbal S. Berry-Cabán, Y. Sammy Choi (2014) Report of a False Positive Rapid HIV Test Due to Hepatitis A in a U.S. Army Soldier. Journal of Clinical Research In HIV AIDS And Prevention - 2(2):13-17.

Download as RIS, BibTeX, Text (Include abstract )

DOI 10.14302/issn.2324-7339.jcrhap-14-541


Human immunodeficiency virus (HIV) is a well-known sexually transmitted disease that accounts for significant morbidity and mortality. A report by the Centers for Disease Control and Prevention (CDC) approximated 1,144,400 cases of HIV in 2010 in the United States with about 21,278 cases of death from known HIV.1

Although HIV in the military is not of particular concern, all active duty personnel are required to have biennial HIV testing unless they are already HIV-positive. From 2008 to 2012, the incidence of HIV diagnosis among U.S. Army active component increased 22%. In 2012, 416,715 soldiers were tested and 111 (108 male) were found to be HIV-positive.2

Rapid HIV antibody testing allows for obtaining preliminary results within 20 to 30 minutes. This is advantageous in many populations including the military where early diagnosis can positively affect soldier and unit readiness. If positive, a rapid test is only considered a “preliminary positive” as confirmatory immunoassays and Western blots are necessary.3 Like conventional screening antibody immunoassays, a positive rapid result requires confirmatory testing. As with all screening tests, false-positive results occur. We describe the first case report of a false positive HIV antibody test due acute hepatitis A infection.

Materials and Methods


A 25 year old, single, white, male Army soldier presented to a clinic in Afghanistan complaining of fatigue, malaise, nausea, chills, and acholic stools. He denied any sexual contact within the previous year and denied alcohol, tobacco, illicit drugs or supplement use. His only medication was doxycycline prescribed for malaria prophylaxis for which he was compliant. He worked closely with Afghani soldiers and frequently ate local food.

Physical examination was remarkable for normal vital signs, mild hepatomegaly, and marked jaundice and scleral icterus. Initial laboratory testing was significant for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) at nearly 5000 U/L. Hepatitis B and C testing was negative. HIV testing using OraQuick® Rapid HIV-1 Antibody Test (OraQuick®) (OraSure Technologies, Bethlehem, Pennsylvania, USA) was positive. Prior to deployment, the patient had a negative conventional HIV-1/2 antibody test.

The patient was evacuated to Landstuhl, Germany and then to Womack Army Medical Center (WAMC), Fort Bragg, NC for further evaluation. At WAMC, laboratory investigation included a hemoglobin of 13.8 g/dL, white blood cell count of 4.3 k/uL and platelet count of 213 k/uL. Prothrombin time and activated partial thromboplastin time were normal. Testing for hepatitis B, C, and E virus were negative. Hepatitis A IgM was positive. Conventional HIV enzyme immunoassay (EIA), Western blot and HIV-1 RNA quantitative assay were also negative. No record of hepatitis A vaccination was found. Four months after discharge, his AST and ALT were normal.


In 2013, the US Preventive Services Task Force recommended universal HIV screening among patients age 15-65 years.4 Such screening allows the detection of asymptomatic infected patients thus providing the opportunity to prevent disease transmission and reduce morbidity with early anti-retroviral therapy. Previously, rapid HIV testing was advocated by the Centers for Disease Control and Prevention in their initiative, “Advancing HIV Prevention: New Strategies for a Changing Epidemic”.5 Early diagnosis can be helpful in several populations to include high risk individuals, especially if they have infrequent follow-up and following accidental needlestick injuries to health care workers. Similarly, for pregnant women with unknown HIV status, rapid diagnosis is crucial for prompt treatment of mothers and infants.

Currently, several approved rapid HIV tests are available to patients: OraQuick® and OraQuick ADVANCE® Rapid HIV-1/2 Antibody Test (OraSure Technologies, Bethlehem, PA, USA); Reveal Rapid HIV-1 Antibody Test, Reveal G2 Rapid HIV-1 Antibody Test, and RevealG3 Rapid HIV-1 Antibody Test (MedMira Laboratories, Inc., Halifax, Nova Scotia); Uni-Gold Recombigen® HIV Test (Trinity BioTech, Bray, Ireland), Multispot HIV-1/HIV-2 Rapid Test (Bio-Rad Laboratories, Redmond, WA, USA), Clearview® HIV 1/2 STAT-PAK, Clearview COMPLETE® HIV1/2 Assay (Alere, Waltham, MA, USA); and Insti HIV-1 Antibody Test Kit (bioLytical Laboratories, Inc., British Columbia). Since this case was conducted, OraQuick® In-Home HIV Test (Orasure Technologies, Bethlehem, PA, USA) and Chembio DPP® HIV 1/2 Assay (Chembio Diagnostic Systems, Inc., Medford, NY, USA) have also been approved. Delaney et al. compared 6 rapid HIV tests to conventional EIAs and found all to have statistically similar high specificity and sensitivity.6 The OraQuick ADVANCE®, which is the only FDA-approved rapid test for oral fluid (can also be used with whole blood or plasma), had a sensitivity of 99.3% and specificity of 99.8% for oral specimens.6

A positive rapid HIV antibody test requires confirmation. Our patient, after having a positive screening test, had conventional EIA and confirmatory Western blot/HIV RNA testing that was negative. Hence he was assessed as having a false-positive rapid test due to acute hepatitis A infection.

A review of the literature reveals that false-positive conventional EIA testing has been reported to be associated with a variety of states to include vaccinations with rubella,7 influenza8, 9, rabies10, hepatitis B11, and tetanus;12 acute cytomegalovirus infection;13 systemic lupus erythematosis;14 and the presence of rheumatoid factor or anti-nuclear antibody.12 For the military who deploy to numerous continents to include Africa, it is important to be aware that false-positive EIA testing can also occur with malaria,15 human African trypanosomiasis,16 visceral leishmaniasis,17trypanosoma cruzi,18 and schistosomiasis.19 An important cause of false-positive test in the US is pregnancy. Chao et al. reviewed data from 47,794 pregnant women and found that 0.3% had a false positive test. The positive predictive value was 54.3%.20 Younger and nulliparous women were more likely to have a false positive. Of note, Tung21 and Jamieson22 have shown that the false-positive rate among pregnant women is significantly lower using OraQuick® than with conventional EIA testing.

For false positive rapid tests, the literature documents reports only for OraQuick®. In January 2004, New York City started offering on-site rapid HIV testing of finger-stick whole blood with OraQuick®. In March 2005, they switched to oral fluid testing using OraQuick ADVANCE®. In late 2005 and 2007, an unexpected increase in false-positive oral fluid rapid tests occurred; no clear cause was found.23 Similarly, Jafa et al. reported on an investigation of 16 false positive oral specimens using the OraQuick ADVANCE®; no cause was identified.24

False-positive OraQuick ADVANCE® results have been caused by dengue fever25 and have been associated with O-negative blood and sex with an HIV-infected person.26 Additionally, the package labeling for OraQuick® Rapid lists several potentially interfering medical conditions that are associated with false-positive testing: multiparous women, rheumatoid factor, Epstein Barr virus, and hepatitis A and B viruses.27

For many of these conditions, infection and subsequent antibody response may result in cross-reactivity that produces a false-positive test.28


HIV diagnosis in a time-efficient manner is readily available with rapid HIV testing. Upon diagnosis, a treatment regimen can begin earlier than determined by traditional testing methods. The benefits and timeliness of rapid tests for HIV diagnosis has led to their increased use, now including home testing. Physicians and health care providers should be aware that while these tests have high sensitivities and specificities, they can also falsely detect HIV antibody. All positive rapid HIV tests require confirmatory testing. If proven negative, clinicians should investigate for possible etiologies.


The authors declare that there are no sources of funding regarding the publication of this paper.

Written consent for this case report was received from the patient and is available for review by the editorial office.

The views expressed herein are those of the authors and do not reflect the official policy of the Department of the Army, Department of Defense, or the U.S. Government.


  1. 1. (2013) Centers for Disease Control and Prevention. Monitoring selected national HIV prevention and care objectives by using HIV surveillance data - United States and 6 dependent areas-2011.In: HIV Surveillance Supplemental Report.
  1. 2. (2013) Armed Forces Health Surveillance Center. Update: Routine Screening for Antibodies to Human Immunodeficiency Virus, Civilian Applicants for U.S. Military Service and U.S. Armed Forces, Active and Reserve Components,January2008-June2013. , Medical Surveillance Monthly Report 20, 2-9.
  1. 3.Greenwald J L, Burstein G R, Pincus J, Branson B. (2006) A rapid review of rapid HIV antibody tests. , Current Infectious Disease Reports 8, 125-31.
  1. 4.Moyer V. (2013) Screening for HIV: U.S. Preventive Services Task Force recommendation statment. , Ann Intern Med 159, 51-60.
  1. 5. (2003) Centers for Disease Control and Prevention. Advancing HIV Prevention: new strategies for a changing epidemic-United. , States,2003.MMWR 52, 329-32.
  1. 6.Delaney K P, Branson B M, Uniyal A. (2011) Evaluation of the performance characteristics of 6 rapid HIV antibody tests. Clinical infectious diseases 52:. 257-63.
  1. 7.Araujo P, Albertoni G, Arnoni C. (2009) Rubella vaccination and transitory false-positive test results for human immunodeficiency virus type 1 in blood donors. , Transfusion 49, 2516-7.
  1. 8.Simonsen L, Buffington J, Shapiro C. (1995) Multiple false reactions in viral antibody screening assays after influenza vaccination. , Am J Epidemiol 141, 1089-96.
  1. 9.Mac Kenzie WR, Davis J P, Peterson D E. (1992) Multiple False-positive Serologic Tests for HIV, HTLV-1, and Hepatitis C Following Influenza Vaccination,1991.JAMA 268:. 1015-7.
  1. 10.Pearlman E, Ballas S. (1994) False-positive human immunodeficiency virus screening test related to rabies vaccination. , Arch Path Lab Med 118, 805-6.
  1. 11.Lee D, Eby W, Molinaro G. (1992) HIV false positivity after hepatitis B vaccination. , Lancet 339, 1060.
  1. 12.Celum C L, Coombs R W, Jones M. (1994) Risk factors for repeatedly reactive HIV-1 EIA and indeterminate Western blots: a population-based case-control study. , Archives of internal medicine 154, 1129.
  1. 13.Bronze M S, Warr A G, Spigel D, Smith V D, Smalley D. (1998) False-positive enzyme immunoassay for human immunodeficiency virus due to acute cytomegalovirus infection. Clinical infectious diseases 27:. 221-2.
  1. 14.Esteva M, Blasini A, Ogly D, Rodriguez M. (1992) False positive results for antibody to HIV in two men with systemic lupus erythematosus. , Ann Rheum Dis 51, 1071-3.
  1. 15.Gasasira A, Dorsey G, Kamya M. (2006) False-positive results of enzyme immunoassays for human immunodeficiency virus in patients with uncomplicated malaria. , J Clin Microbiol 44, 3021-4.
  1. 16.Lejon V, Ngoyi D, Ilunga M. (2010) Low specificities of HIV diagnostic tests caused by Trypansoma brucei gambiense sleeping sickness. , J Clin Microbiol 48, 2836-9.
  1. 17.Salinas A, Gorgolas M, Fernandez-Guerro M. (2007) Refrain from telling bad news: patients with Leishmaniasis can have false-positive HIV test results. , Clin Infect Dis 45, 139-40.
  1. 18.Parra-Piñeros J, Valderrama V, Leon-Sarmiento F. (2004) False-positive human immunodeficiency virus test and Trypanosoma cruzi infection in eastern Colombia. , South Med J 97, 423-4.
  1. 19.Everett D, Baisely K, McNerney R. (2010) Association of Schistosomiasis with false-positive HIV test results in an African adolescent population. , J Clin Microbiol 48, 1570-7.
  1. 20.Chao T T, Sheffield J S, Wendel G D, Ansari M Q, McIntire D D et al. (2012) Risk Factors Associated with False Positive HIV Test Results in a Low-Risk Urban Obstetric Population. , Journal of Pregnancy2012
  1. 21.Tung C, Sangi-Haghpeykar H, Levison J. (2010) Rapid versus standard testing for prenatal HIV screening in a predominately Hispanic population. , J Perinatol 30, 30-2.
  1. 22.Jamieson D, Cohen M, Maupin R. (2007) Rapid human immunodeficiency virus-1 testing on labor and delivery in 17 US hospitals: the MYRIAD experience. , Am J Obstetr Gynecol 197, 72-82.
  1. 23.Cummiskey J, Mavinkurve M, Paneth-Pollak R. (2008) False-positive oral fluid rapid HIV tests-New York City,2005-2008.MMWR Morb Mortal Wkly Rep. 57, 660-5.
  1. 24.Jafa K, Patel P, MacKellar D A. (2007) Investigation of false positive results with an oral fluid rapid HIV-1/2 antibody test. , PLoS One 2, 185.
  1. 25.Mao A, Obi-Gwacham C, Hill T, Ryan C, Hendricks A et al. (2010) Acute dengue fever causes false-positive reactivity in OraQuick rapid HIV-1/2 antibody test. , J Acquir Immun Defic Syndr 55, 641.
  1. 26.Rifkin S B, Owens L E, Greenwald J L. (2012) Factors Associated with False-Positive Results from Fingerstick OraQuick. , ADVANCE Rapid HIV 1/2 Antibody Test. J Int Assoc Phy AIDS Care 11, 356-60.
  1. 27. (2012) OraQuick Rapid HIV-1 Antibody Test [package insert]. , Bethlehem, PA:, OraSure Technologies, Inc.;. Available
  1. 28.Klarkowski D, O'Brien D, Shanks L, Singh K. (2014) Causes of false-positive HIV rapid diagnostic test results. , Expert Rev Anti Infect Ther 12, 49-62.

Cited by (1)

  1. 1.Elsner Carina, Appeltrath Gwenllian A., Konik Margarethe, Parreuter Janine, Broecker-Preuss Martina, et al, 2023, False-Positive Screening and Confirmatory HIV Diagnostic Test in a Patient with Cured SARS-CoV-2 Infection Is Not Mediated by Env/Spike Cross-Reactive Antibodies, Viruses, 15(5), 1161, 10.3390/v15051161