The authors have declared that no competing interests exist.
44fungal species were isolated from plant parts submerged in Al-Huwaiza marsh within Iraqi borders, and 7 new first records fungi in Iraq were isolated too, which have been illustrated and described as follows:-
Carbosphaerella leptosphaeriodes, Curvularia lunata var.aria, Graphium sp., Helicascus kanaloanus, Leptosphaeria obions, Stagnospora sp. and Ulocladium tuberculatum, Carbosphaerella leptosphaeriodes, Curvularia lunata var. aeria, Graphium sp., Helicascus kanaloanus, Leptosphaeria obions, Stagnospora sp., Ulocladium tuberculatum.
Al-Huwaizah marsh is an aquatic ecosystem extend between Iraq and Iran with freshwater body. Al-Huwaizah marsh locates between latitudes 31˚ 45ˉ and 31˚ 00ˉ in the north and longitude 47˚ 50ˉ and 47˚ 25ˉ in the east, passing through Iranian borders, 80 km X 30 km, (Scott, 1995)
Hussein Al-Nasrawi (2006) was confirmed isolation of fungal diversity (fifteen species) as a new record for the first time in Iraq, isolated from the plant remains submerged in aquatic ecosystems in Iraq , in addition to many studies conducted in Iraq for the same mycological puroses (Jaber.,etal.2012; Graca et al., 2016)
50 pieces of decomposed plants were collected from water body and sediments in Al Huwaizah marsh in Iraq during 2016. Samples were washed gently by tap water and then by distilled water . Plant debris were cut into small parts 7 - 5 cm long and and each 10 pieces were settled in the bottom of petri dish.
Potato Carrot Agar (PCA), which was obtained by weighing 20 g of potato and carrots after washing and peeling, then sliced and boiled with a quantity of distilled water, was sprayed well in a ceramic vase, filtered and placed in a 1 liter flask then added to the prepared mixture Of each of the potatoes and carrots media. the media objected to sterilizing process in autoclave under standard conditions for 20 minutes (250mg 0f chloramphenicol as antibiotic to inhibit bacterial growth.
In this study, two methods were used to isolate the fungi: direct isolation from the substrate. The humid chamber method was used to remove the previously prepared vegetable pieces from the beaker using sterile forceps and placed 7 to 5 pieces in a glass bowl of 15 cm diameter Petri dishes Sterilize the filter leaves, then moisten the filter leaves with sterilized distilled water and incubate the dishes under 25° C. The second method is the method of dilution Dilution method to isolate the fungus from the washing of submerged plant parts and summarized the method by withdrawing 10 ml of sterile distilled water, which was washed by the samples previously using a sterile pipette placed in a flask containing 90 ml of distilled distilled water and a well well and withdraw from it 1 ml transferred to A sterile glass dish with a diameter of 9 cm. The food medium, plate roast and incubation were incubated under 25° C. Three replicates were made of each sample.
The isolated fungi were classified under light microscope by using international taxonomic keys published in the following literatures: Arx etal., (1986); Boerema, G.H.(1993); Booth, (1971); Cai etal., (2003); Ellis, (1971); Ellis, (1976) ;Gohand Clement,(2003); Hoog and Guarro,(1995) ; Kohlmeyer and Kohlmeyer,( 1979); Kohlmeyer and Kohlmeyer, (1991); Pitt, (979). Sivavanesan, ( 1987).
The total fungal species isolated from Al-huwaizah marsh.
Ascocarp 90-120 um in diameter, globose to subglobose shape . The Asci 40-45 X 60-80 um with 8 Ascospores. The Ascospore 15-18X 25-30 um, devided by triseptate, the two mid cells within the ascospore dark to brown color, whereas the terminal cells pale and surrounded by mucous sheath. The present isolate nearly like the isolate of Schmidt, 1969.This species consider as a new record for Iraq. The isolate was illustrated and kept in Basra herbarium under no. BASRA 2011. (Figure 1).
The colony with black to gray color, The hyphae immersed under substrate surface . Conidiophore thicker than fungal filament (macronematous), subhyaline. Conidiogenous cell is polytretic. The conidia with curve shape divided with three septae to form four cells, the two mid cells thicker and darker from the two terminal pale cells . Conidia 10-15X 20-30 um. This species was previously isolated from painted wood and soil whereas our present fungus isolated from reed sample submerged in marsh sediments. Dry culture was kept in Basra herbarium under no. BASRA 2012. (Figure 2).
The colony is gray to Olivaceous brown, Conidiophore thicker than fungal filament (macronematous) appears under dissecting microscope as Synnemata. Fungal hayphae immersed under the epidermis. Conidiogenous cell is monobasic type. Conidia 5-7X 15-20 um. Oval to cylindrical shape, with rounded end, pale color without, unseptated. Our isolated fungus resemble species Graphium putredinis isolated by Huges, 1958
The Ascocarp globose , immersed , 400-250 um high, 400-800 with ostule. Black to dark brown color. Asci 200-300 um., bitunicate, with 8 ascospores. The ascospore 15-25X35-50 um. Arranged inside ascus as uniseriate. The ascospore devided by septum into 2 dark cells, with funnel shape. cell wall of ascospore surrounded by two layers. There are two germination pores in the ends of ascospore. The ascospore differentiated by gelatinous layer clearly appears when immerse in water drop (disappear with lactophenol stain). The present fungus isolated from Typha segment submerged in marsh sediments , illustrated and kept in Basra herbarium under no. BASRA 2014. (Figure 4)
Ascocarp sub globose, immersed, with high about 100-300 and diameter 200-400 um., black to dark brown color, usually covered by brown filaments. The ascocarp coated by two layers , large dark external layer and pale small internal layer. The asci thick, bitunicate,14-20 X 150-300 um. Each ascus contain 8 ascospores, 8-15X 25-40 um. Arranged inside the ascus as uniseriate in the top of the ascus wherease as biseriate in middle site. The ascospore divided by three septae to form 4 cells, the two middle cells dark brown and larger than the terminal smallest cells. This species was previously isolated from hrbal plants and from mangrove area in Australia .The present fungus isolated from typha segment submerged in marsh sediment, illustrated and kept in Basra herbarium under no. BASRA 2015. (Figure 5)
The Pycnidium sub globose, partialy immersed, with pale brown ostiole and short papillate.High of pycnidium 150-180 um, 100-200 um diameter with a neck about 10 um diameter. The conidia pale to brown color, cylindrical in shape, 4- 8 X 40-70 um., divided by 5-7 septae .The present isolate ressemble Stagonospora haliclysta which was previously isolated by Kohlm, 1973 (conidia size 3.5-4.5X 20-27 um., smaller than our isolate). The present fungus isolated from reed segment submerged in marsh sediment, and dconsider as first record in Iraq. It was illustrated and kept in Basra herbarium under no. BASRA 2016. (Figure 6)
The fungal hyphae immersed, sub hyaline, with thick conidiophore 4-6 um. 160-200 um. length, pale brown color, divided by septae. Conidia 10-16 X 10-20 um. sub globose, like potato fruit, divided into several parts by septae cross shape
The species was firstly isolated in united states. The present isolate ressemble Tetracoccosporium paxianum, which isolated by Szabo, 1905. Our species was isolated from reed segment submerged in marsh sediment, kept in Basra herbarium under no. BASRA 2017. (Figure 7).
Fungi Inhabit plant segments submerged in aquatic ecosystems used their enzymic complex system to biodegrade cellulose and produce carbon source, the most important matter for fungal metabolism process (Gessner, 1980; Garraway and Evans, 1984)
Guaro et al., 1996, Abdullah et al., 1996 and Guaro et al., 1997
Several marshed in sothern area of Iraq still waiting more studies and novel works to discover more new species and new record fungi. The high quality water parameters of marsh ecosystem with enrichment of plant diversity, leads to establishment a perfect foundation of sediment layers embedded in the bottom of marsh environment, This study open the track for researchers to investigate the ecological niche of fungi in marsh community to detect more aquatic and sediment mycoflora of wetlands.