Preoperative and Postoperative N-terminal Pro B-type Natriuretic Peptide Levels Predict Cardiac Morbidity and Mortality in Patients Undergoing Colorectal Cancer Resection

Introduction: Cardiac complications are a major cause of perioperative morbidity and mortality in patients undergoing colorectal cancer surgery. A quick and reliable system for predicting postoperative cardiac morbidity is needed to predict cardiac events in order to improve outcome in surgery. The aim of this study was to investigate the role of the biochemical marker NT-proBNP in the prediction of postoperative all-cause mortality, cardiac-related mortality and cardiovascular events in patients undergoing colorectal cancer resections. Methods: 100 consecutive patients undergoing colorectal cancer surgery were prospectively recruited. Blood samples were taken preoperatively, 24h, 48h and 5-7 postoperative days to measure NT-proBNP levels. The predictive power of NT-proBNP levels was assessed using Receiver Operating Characteristics (ROC) curves. Results: Cardiac-related morbidity and mortality was 9%. Of eleven deaths, 5 were cardiac-related. Preoperative NT-proBNP was a good predictor of death with ROC area under curve (AUC) of 0.83 (95% C.I. 0.673, 0.993) a strong predictor of cardiac death with AUC of 0.914 (95% C.I. 0.823, 1.000) and a good predictor of cardiac complications with AUC of 0.875 (95% C.I. 0.757, 0.993). NT-proBNP levels 24 hours and 48 hours postoperatively were also strongly predictive of postoperative cardiac morbidity and mortality. Conclusion: Preand postoperative NT-proBNP have a role in predicting postoperative death and cardiac complications. This may have significant implications in the planning of postoperative care for high-risk patients. DOI : 10.14302/issn.2471-7061.jcrc-18-2446 Freely Available Online www.openaccesspub.org | JCRC CC-license DOI : 10.14302/issn.2471-7061.jcrc-18-2446 Vol-1 Issue 3 Pg. no.2 Introduction Cardiac complications are a major cause of perioperative morbidity and mortality in patients undergoing colorectal cancer surgery.The prediction of these cardiac events is notoriously difficult, but essential, as determining the stage of heart disease may lead to different management choices, such as angiotensin converting enzyme inhibitors or b blockers. These treatments can delay or reverse progression of disease and therefore may improve prognosis by reducing postoperative complications. One important aim of preoperative evaluation is to identify patients with significant coronary artery disease who may be at risk of cardiac complications following surgery. Many studies have looked into factors that might adversely affect these cardiac events and they include increasing age, New York Heart Association class, left ventricular ejection fractions, diabetes mellitus and lower blood pressure. Some of these risk indices have been developed into preoperative cardiac risk scoring systems such as revised cardiac risk index (RCRI) and Goldman cardiac index. Other methods of assessing cardiac risks such as dobutamine cardiac stress testing and echocardiography are highly sensitive but are expensive and often limited by time and availability of resources. Furthermore, the Government has set a challenging target for the NHS, that by December 2008, no patient should wait more than 18 weeks from the time of GP referral to the start of hospital treatment. These tests could potentially delay surgery and would therefore not be suitable for pre-operative surgical patients. A quick and reliable system for predicting postoperative cardiac morbidity is needed to predict cardiac events in order to improve the outcome of surgery. Recent published literature has indicated that Brain Natriuretic Peptide (BNP) and its related compound, N-terminal fragment pro B-type Natriuretic Peptide (NT-proBNP), can be used to identify patients at high risk of developing major adverse cardiac events following non-cardiac surgery. BNP is synthesized by human cardiac myocytes as a 108-amino acid prohormone (proBNP), which is cleaved to the 32-residue BNP and the 76-residue NT-proBNP. Both BNP and NT-proBNP can be used as sensitive biomarkers of cardiac dysfunction and have been used as preoperative predictors of cardiac morbidity in cardiac and non-cardiac surgery[6, 9, 10]. The biochemical markers BNP and NT-proBNP are available as commercial assays. The aim of this study was to investigate the role of the biochemical marker NT-proBNP in the prediction of postoperative all-cause mortality, cardiac-related mortality and cardiovascular events in patients undergoing colorectal cancer resections.


Introduction
Cardiac complications are a major cause of perioperative morbidity and mortality in patients undergoing colorectal cancer surgery. 1 The prediction of these cardiac events is notoriously difficult, but essential, as determining the stage of heart disease may lead to different management choices, such as angiotensin converting enzyme inhibitors or b blockers 2 .
These treatments can delay or reverse progression of disease and therefore may improve prognosis by reducing postoperative complications.
One important aim of preoperative evaluation is to identify patients with significant coronary artery disease who may be at risk of cardiac complications following surgery.Many studies have looked into factors that might adversely affect these cardiac events and they include increasing age, New York Heart Association class, left ventricular ejection fractions, diabetes mellitus and lower blood pressure. 3Some of these risk indices have been developed into preoperative cardiac risk scoring systems such as revised cardiac risk index (RCRI) 4 and Goldman cardiac index. 5Other methods of assessing cardiac risks such as dobutamine cardiac stress testing and echocardiography are highly sensitive but are expensive and often limited by time and availability of resources.Furthermore, the Government has set a challenging target for the NHS, that by December 2008, no patient should wait more than 18 weeks from the time of GP referral to the start of hospital treatment.These tests could potentially delay surgery and would therefore not be suitable for pre-operative surgical patients.A quick and reliable system for predicting postoperative cardiac morbidity is needed to predict cardiac events in order to improve the outcome of surgery.
Recent published literature has indicated that Brain Natriuretic Peptide (BNP) and its related compound, N-terminal fragment pro B-type Natriuretic Peptide (NT-proBNP), can be used to identify patients at high risk of developing major adverse cardiac events following non-cardiac surgery. 6BNP is synthesized by human cardiac myocytes as a 108-amino acid prohormone (proBNP), which is cleaved to the 32-residue BNP and the 76-residue NT-proBNP. 7Both BNP and NT-proBNP can be used as sensitive biomarkers of cardiac dysfunction 7 and have been used as preoperative predictors of cardiac morbidity in cardiac 8 and non-cardiac surgery [6, 9, 10].The biochemical markers BNP and NT-proBNP are available as commercial assays.
The aim of this study was to investigate the role of the biochemical marker NT-proBNP in the prediction of postoperative all-cause mortality, cardiac-related mortality and cardiovascular events in patients undergoing colorectal cancer resections.

Methods
The study was approved by the regional ethics committee and hospital research and development department.Between September 2006 and October 2007, 100 consecutive patients undergoing elective colorectal cancer resection who gave consent were recruited to the study.All patients had preoperative cardiac risk stratification using the revised cardiac risk index, which stratifies patients into classes I -IV. 4

Results
Of the 100 patients recruited, 66 were male and 34 were female, with a median age of 72 years (range 39-90).Table 2 shows the different types of colorectal operations performed along with their postoperative histology results.

Adverse Outcomes
Of the 100 patients, there were 11 postoperative deaths, of which 5 were due to cardiac causes.These included 4 myocardial infarctions and one patient with acute left ventricular failure and atrial fibrillation.There were 9 patients with cardiac complications including the 5 cardiac-related deaths.However, this has not been formally assessed in a cost-benefit analysis.Previous work on higher risk patients, undergoing elective and emergency aortic aneurysm repair, has yielded much higher proportions of patients having Tn-T rises postoperatively. 17There may be more justification in routine post operative Tn-T measurement in these patients.
The study has several limitations.Firstly, it was carried out in one centre, so it may not be possible to apply the results of this study to another population because of different prevalence of cardiac disease.
Secondly data on confounding factors such as intraoperative blood loss and surgical contamination was not collected in this study.These could potentially heavily influence the outcome after surgery.
Remarkably, there were no anastomotic leaks diagnosed in this series of patients.It is possible that some of these cardiac complications could be attributed to

1 Table 3 .
The distribution of cardiac complications, cardiac deaths and other deaths according to risk stratification using the Revised Cardiac Risk Index (RCRI) high-risk type of surgery, history of ischemic heart disease, history of congestive heart failure, history of cerebrovascular disease, preoperative treatment with insulin, and preoperative serum creatinine >2.0 mg/dL Table 1.Cardiac risk in Non-cardiac surgery according to the revised cardiac risk index 4 Freely Available Online www.openaccesspub.org| JCRC CC-license DOI : 10.14302/issn.2471-7061.jcrc-18-2446Vol-1 Issue 3 Pg.no.-4at days 1, 2 and 5-7.To avoid bias, all tests and clinical assessments were performed by a clinical researcher who was not involved in patients' care.Cardiac complications included acute myocardial infarction, silent myocardial infarction, acute coronary syndrome, heart failure and cardiac arrhythmias.The diagnosis of heart failure required a clinical diagnosis of heart failure and radiological evidence of pulmonary oedema.All ECGs were read by an experienced consultant cardiologist.Where cardiac complications were identified by the researcher, this was communicated to the clinical team looking after the patient for immediate referral to a cardiologist.Data were also prospectively collected on type of resection undertaken, postoperative histology and postoperative complications.Surgery was carried out by one of four colorectal firms in all cases.Assay Serum NT-proBNP levels were measured using a sandwich immunoassay principle utilising a biotinylated antibody; streptavidin-coated and streptavidin-coated microparticles, with electrochemical detection of the bound complex via the rare earth ruthenium.The normal NT-proBNP value using this assay is <40 pmol/L.Statistics The value of preoperative NT-proBNP, 24 hours postoperative NT-proBNP and 48 hours postoperative NT-proBNP levels as predictors for overall death, cardiac death and cardiac complications were assessed using Receiver Operator Characteristic (ROC) curves.The area under each ROC curve (i.e.AUC) was calculated.A perfect predictive score will be equal to one.A score showing good predictive value will have a value > 0.8.The sensitivities, specificities, negative predictive values (npv) and positive predictive values (ppv) were also calculated for NT-proBNP value cut-off levels of 40 pmol/ L and 90 pmol/L.
[12-14 ]Patients having both cardiac and vascular surgery have high rates of cardiac disease and the role of BNP as a predictor of postoperative events in high risk cardiac patients having non-cardiac surgery has previously been described.15This study has demonstrated that both pre-operative and postoperative measurement of NT-proBNP can help in identifying patients at high risk of major adverse events following colorectal cancer surgery.Pre-operative NT-proBNP levels may be able to predict the pre-operative cardiac status of patients but do not necessary reflect the variable dynamic sequences of postoperative stress responses which may result in adverse cardiac events.On the other hand, the postoperative measurement of NT-proBNP takes these factors into consideration, particularly with respect to the risk of cardiac death or cardiac complications.The use of NT-proBNP as a predictivepreoperative biochemical marker has significant potential clinical applications.It is envisaged that patients with high preoperative NT-proBNP levels, indicating a high risk of postoperative cardiovascular events, could be referred for cardiac optimisation.Valuable hospital resources, such as the use of intensive care monitoring, can also be targeted for patients who fall in this group.It remains to be seen whether such interventions wouldhave any effect on patient morbidity or mortality.In our study, the greatest power of the preoperative NT-proBNP value was as a negative predictor.This means that normal NT-proBNP values were associated with low risks of cardiac events.This seems to correlate with the results found in heart failure where the European Society of Cardiology Task force guidelines for the treatment of chronic heart failure state that natriuretic peptides "may be most useful clinically as a rule out test due to consistent and very high negative predictive values."16A potential application of this test would be to identify patients not requiring high dependency or intensive care post-operatively from the point of view of their cardiac function, if their pre-operative NT-proBNP levels were less than 90pmol/l.In our study, 11 patients (11%) had a rise in Tn-T post-operatively, 6 of which were not apparent clinically and 2 of which were diagnosed as silent myocardial infarctions.These 2 patients were referred to cardiologists and treated for myocardial infarction, with appropriate cardiology follow-up.The routine measurement of post operative Tn-T, although useful for these 2 patients, may not be justified in the routine care of these patients when the cost of the test is considered.
www.openaccesspub.org| JCRC CC-license DOI : 10.14302/issn.2471-7061.jcrc-18-2446Vol-1 Issue 3 Pg.no.-8 undiagnosed leaks, as one previous study demonstrated that approximately 40% of patients having cardiac symptoms following a restorative colorectal resection, in fact had suffered an anastomotic leak. 18The NT-proBNP test is safe and convenient, requiring only a routine venepuncture.Currently a routine NT-proBNP test will cost the NHS £25.00 per patient and this is relatively cheap to screen some of the high-risk patients.However, there are no data to support the use of NT-proBNP in the general screening of asymptomatic populations for heart failure prior to surgery.What is clear from our study is that NT-proBNP measurements are useful as an adjunct to other clinical tools to determine the risks of patients undergoing non-cardiac surgery.With the current emerging evidence, NT-proBNP has the potential to be used in clinical practice.Patients with high risks of cardiovascular disease should have a screening NT-proBNP blood test.Patients with elevated NT-proBNP should be referred to a cardiologist for consideration of additional stress testing or echocardiogram, to assess the presence and extent of coronary artery disease.Conversely patients with normal NT-proBNP tests have the potential to avoid unnecessary patient referral for echocardiography or stress testing.